Abstract
Experiments were conducted to evaluate health of fish, Channa punctatus inhabiting mercuric chloride. Acute toxicity bioassays were performed to calculate 96h LC50 value and it was found to be 1.38mg/L. Chronic toxicity assay to investigate genotoxic effects on the erythrocytes of fish by comet assay and micronucleus assay along with alterations in blood biochemistry were evaluated. Results showed concentration and duration dependent significant DNA damage as observed by comet assay and micronucleus assay. The frequency of nuclear aberrations along with appearance of micronuclei were observed after 30 and 60 days of exposure. The blood biochemistry was studied by recording changes in levels of various biochemical parameters in blood serum and results showed the significant (p < 0.05) variations among levels of biochemical constituents such as Glucose, Lipids, Proteins, Bilirubin, Urea, Creatinine, Cholesterol, ALP, Albumin, SGOT, SGPT and Total Glycerides. The resultsindicated the stress response of specimen towards toxicant. The present study highly recommends the use of genotoxicity and blood biochemical analysis as the useful biomarker to assess toxicity in the aquatic water and hence to safeguards the surrounding ecosystem.
Geno and cytotoxicologic assessment of wastewater effluents with mussel micronucleus assay and with flow cytometric sperm toxicity assay a comparison
