scholarly journals Immunoblot analysis of horseradish peroxidase conjugates of wheat germ agglutinin before and after retrograde transport in the rat peripheral nervous system

1985 ◽  
Vol 5 (10) ◽  
pp. 2779-2785 ◽  
Author(s):  
ML Schmidt ◽  
JQ Trojanowski
Keyword(s):  
Nervous System ◽  
Horseradish Peroxidase ◽  
Peripheral Nervous System ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Retrograde Transport ◽  
Immunoblot Analysis ◽  
Before And After

scholarly journals Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport.

1979 ◽  
Vol 27 (3) ◽  
pp. 728-734 ◽  
Author(s):  
N K Gonatas ◽  
C Harper ◽  
T Mizutani ◽  
J O Gonatas
Keyword(s):  
Horseradish Peroxidase ◽  
Axonal Transport ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Brain Research ◽  
Retrograde Transport ◽  
Retrograde Axonal Transport ◽  
Hypoglossal Nucleus ◽  
Highly Sensitive ◽  
Cervical Ganglion

We have compared the retrograde axonal transport of horseradish peroxidase (HRP), to the retrograde transport of HRP conjugated with wheat germ agglutinin (WGA). Morphometric studies have shown that WGA-HRP conjugates were 40 times more sensitive than free HRP, in the tracing of retrograde connections from the rat submandibular gland to the superior cervical ganglion. Also, WGA-HRP was more sensitive than free HRP in the tracing of retrograde connections from the rat tongue to the hypoglossal nucleus. Our findings with WGA-HRP are consistent with the observations by Schwab et al. who reported (-125I) WGA is a highly sensitive retrograde tracer (Brain Research 152:145, 1978 (22)).

Selective vagal rhizotomies: a new dorsal surgical approach used for intestinal deafferentations

1995 ◽  
Vol 269 (5) ◽  
pp. R1279-R1288 ◽  
Author(s):  
E. K. Walls ◽  
F. B. Wang ◽  
M. C. Holst ◽  
R. J. Phillips ◽  
J. S. Voreis ◽  
...  
Keyword(s):  
Body Weight ◽  
Horseradish Peroxidase ◽  
Minimally Invasive ◽  
Surgical Approach ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Daily Intake ◽  
Intracranial Surgery ◽  
Before And After ◽  
New Surgery

We have developed a dorsal intracranial surgery that is minimally invasive and gives excellent access to either afferent or efferent vagal rootlets to produce selective deafferentations or deefferentations in the rat. We have combined this new unilateral afferent rhizotomy with a contralateral celiac branch cut (to completely deafferent the intestines) and a duodenal catheter placement 4 cm distal to the pylorus. Animals were maintained with 17 h/day access to a nutritionally complete liquid diet. Measures of first meal size, daily intake, and body weight before and after both surgeries indicated that animals with unilateral vagal deafferentiations recovered as fast and completely as sham-operated controls. Intraduodenal oleate (1.2 kcal) infusions reduced the size of the first meal in surgical controls (by 64%; P < 0.01) but not in the deafferented rats. A dual wheat germ agglutinin-horseradish peroxidase/Fluorogold protocol provides verification of sensory and motor lesions. The selective vagal deafferentation provided by the new surgery offers a useful model for determining gastrointestinal sites of nutrient detection and separating pre- and postabsorptive consequences of a meal.

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