scholarly journals Ric-8a, a Guanine Nucleotide Exchange Factor for Heterotrimeric G Proteins, Regulates Bergmann Glia-Basement Membrane Adhesion during Cerebellar Foliation

2012 ◽  
Vol 32 (43) ◽  
pp. 14979-14993 ◽  
Author(s):  
S. Ma ◽  
H. J. Kwon ◽  
Z. Huang
Keyword(s):  
Basement Membrane ◽  
G Proteins ◽  
Guanine Nucleotide Exchange Factor ◽  
Bergmann Glia ◽  
Guanine Nucleotide ◽  
Heterotrimeric G Proteins ◽  
Nucleotide Exchange ◽  
Guanine Nucleotide Exchange ◽  
Nucleotide Exchange Factor ◽  
Membrane Adhesion

scholarly journals Activation of Rac1 by the Guanine Nucleotide Exchange Factor Dck1 Is Required for Invasive Filamentous Growth in the Pathogen Candida albicans

2008 ◽  
Vol 19 (9) ◽  
pp. 3638-3651 ◽  
Author(s):  
Hannah Hope ◽  
Stéphanie Bogliolo ◽  
Robert A. Arkowitz ◽  
Martine Bassilana
Keyword(s):  
Candida Albicans ◽  
G Proteins ◽  
Morphological Changes ◽  
Guanine Nucleotide Exchange Factor ◽  
Filamentous Growth ◽  
Guanine Nucleotide ◽  
Nucleotide Exchange ◽  
Exchange Factor ◽  
Guanine Nucleotide Exchange ◽  
Nucleotide Exchange Factor

Rho G proteins and their regulators are critical for cytoskeleton organization and cell morphology in all eukaryotes. In the opportunistic pathogen Candida albicans, the Rho G proteins Cdc42 and Rac1 are required for the switch from budding to filamentous growth in response to different stimuli. We show that Dck1, a protein with homology to the Ced-5, Dock180, myoblast city family of guanine nucleotide exchange factors, is necessary for filamentous growth in solid media, similar to Rac1. Our results indicate that Dck1 and Rac1 do not function in the same pathway as the transcription factor Czf1, which is also required for embedded filamentous growth. The conserved catalytic region of Dck1 is required for such filamentous growth, and in vitro this region directly binds a Rac1 mutant, which mimics the nucleotide-free state. In vivo overexpression of a constitutively active Rac1 mutant, but not wild-type Rac1, in a dck1 deletion mutant restores filamentous growth. These results indicate that the Dock180 guanine nucleotide exchange factor homologue, Dck1 activates Rac1 during invasive filamentous growth. We conclude that specific exchange factors, together with the G proteins they activate, are required for morphological changes in response to different stimuli.

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