scholarly journals Quantitative Comparison of Glutamatergic and GABAergic Synaptic Vesicles Unveils Selectivity for Few Proteins Including MAL2, a Novel Synaptic Vesicle Protein

2010 ◽  
Vol 30 (1) ◽  
pp. 2-12 ◽  
Author(s):  
M. Gronborg ◽  
N. J. Pavlos ◽  
I. Brunk ◽  
J. J. E. Chua ◽  
A. Munster-Wandowski ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Synaptic Vesicles ◽  
Quantitative Comparison ◽  
Synaptic Vesicle Protein ◽  
Vesicle Protein

scholarly journals Biogenesis of synaptic vesicles in vitro.

1995 ◽  
Vol 130 (5) ◽  
pp. 1041-1049 ◽  
Author(s):  
C Desnos ◽  
L Clift-O'Grady ◽  
R B Kelly
Keyword(s):  
Membrane Proteins ◽  
Cell Surface ◽  
Synaptic Vesicle ◽  
Synaptic Vesicles ◽  
Vesicle Membrane ◽  
Synaptic Vesicle Protein ◽  
Vesicle Biogenesis ◽  
Vesicle Protein ◽  
Correct Size

Synaptic vesicles are synthesized at a rapid rate in nerve terminals to compensate for their rapid loss during neurotransmitter release. Their biogenesis involves endocytosis of synaptic vesicle membrane proteins from the plasma membrane and requires two steps, the segregation of synaptic vesicle membrane proteins from other cellular proteins, and the packaging of those unique proteins into vesicles of the correct size. By labeling an epitope-tagged variant of a synaptic vesicle protein, VAMP (synaptobrevin), at the cell surface of the neuroendocrine cell line PC12, synaptic vesicle biogenesis could be followed with considerable precision, quantitatively and kinetically. Epitope-tagged VAMP was recovered in synaptic vesicles within a few minutes of leaving the cell surface. More efficient targeting was obtained by using the VAMP mutant, del 61-70. Synaptic vesicles did not form at 15 degrees C although endocytosis still occurred. Synaptic vesicles could be generated in vitro from a homogenate of cells labeled at 15 degrees C. The newly formed vesicles are identical to those formed in vivo in their sedimentation characteristics, the presence of the synaptic vesicle protein synaptophysin, and the absence of detectable transferrin receptor. Brain, but not fibroblast cytosol, allows vesicles of the correct size to form. Vesicle formation is time and temperature-dependent, requires ATP, is calcium independent, and is inhibited by GTP-gamma S. Thus, two key steps in synaptic vesicle biogenesis have been reconstituted in vitro, allowing direct analysis of the proteins involved.

scholarly journals Rab3C is a synaptic vesicle protein that dissociates from synaptic vesicles after stimulation of exocytosis

1994 ◽  
Vol 269 (15) ◽  
pp. 10971-10974
Author(s):  
G. Fischer von Mollard ◽  
B. Stahl ◽  
A. Khokhlatchev ◽  
T.C. Südhof ◽  
R. Jahn
Keyword(s):  
Synaptic Vesicle ◽  
Synaptic Vesicles ◽  
Synaptic Vesicle Protein ◽  
Vesicle Protein ◽  
Stimulation Of

scholarly journals Botulinum Neurotoxin D Uses Synaptic Vesicle Protein SV2 and Gangliosides as Receptors

2011 ◽  
Vol 7 (3) ◽  
pp. e1002008 ◽  
Author(s):  
Lisheng Peng ◽  
William H. Tepp ◽  
Eric A. Johnson ◽  
Min Dong
Keyword(s):  
Synaptic Vesicle ◽  
Botulinum Neurotoxin ◽  
Synaptic Vesicle Protein ◽  
Vesicle Protein

scholarly journals Synaptic Vesicle Protein 2 (SV2) Isoforms

2012 ◽  
Vol 13 (10) ◽  
pp. 5063-5067 ◽  
Author(s):  
Cindy Bandala ◽  
A. Miliar-Garcia ◽  
C.M. Mejia-Barradas ◽  
M. Anaya-Ruiz ◽  
J.P. Luna-Arias ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Synaptic Vesicle Protein ◽  
Vesicle Protein

Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling

2009 ◽  
Vol 390 (3) ◽  
pp. 415-420 ◽  
Author(s):  
Yukihiro Ohno ◽  
Shizuka Ishihara ◽  
Ryo Terada ◽  
Miki Kikuta ◽  
Nobumasa Sofue ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Synaptic Vesicle Protein ◽  
Vesicle Protein ◽  
Synaptic Vesicle Protein 2A
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