scholarly journals Identification and Precise Mapping of Resistant QTLs of Cercospora Leaf Spot Resistance in Sugar Beet (Beta vulgaris L.)

2011 ◽  
Vol 1 (4) ◽  
pp. 283-291 ◽  
Author(s):  
Kazunori Taguchi ◽  
Tomohiko Kubo ◽  
Hiroyuki Takahashi ◽  
Hideyuki Abe
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Leaf Spot ◽  
Cercospora Leaf Spot ◽  
Precise Mapping

Seed-Borne Cercospora beticola can Initiate Cercospora Leaf Spot from Sugar Beet (Beta vulgaris L.) Fruit Tissue

2021 ◽  
Author(s):  
Rebecca Spanner ◽  
Jonathan Neubauer ◽  
Thies M. Heick ◽  
Michael Grusak ◽  
Olivia Hamilton ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Leaf Spot ◽  
Xylem Sap ◽  
Cercospora Beticola ◽  
Cercospora Leaf Spot ◽  
Internal Transcribed Spacer Region ◽  
Long Distance ◽  
True Seed ◽  
Commercial Sugar

Cercospora leaf spot (CLS) is a globally important disease of sugar beet (Beta vulgaris L.) caused by the fungus Cercospora beticola. Long-distance movement of C. beticola has been indirectly evidenced in recent population genetic studies, suggesting potential dispersal via seed. Commercial sugar beet “seed” consists of the reproductive fruit (true seed surrounded by maternal pericarp tissue) coated in artificial pellet material. In this study, we confirmed the presence of viable C. beticola in sugar beet fruit for 10 of 37 tested seed lots. All isolates harbored the G143A mutation associated with quinone outside inhibitor resistance and 32 of 38 isolates had reduced demethylation inhibitor sensitivity (EC50 > 1 µg/ml). Planting of commercial sugar beet seed demonstrated the ability of seed-borne inoculum to initiate CLS in sugar beet. Cercospora beticola DNA was detected in DNA isolated from xylem sap, suggesting the vascular system is used to systemically colonize the host. We established nuclear ribosomal internal transcribed spacer region amplicon sequencing using the MinION platform to detect fungi in sugar beet fruit. Fungi from 19 different genera were identified from 11 different sugar beet seed lots, but Fusarium, Alternaria, and Cercospora were consistently the three most dominant taxa, comprising an average of 93% relative read abundance over 11 seed lots. We also present evidence that C. beticola resides in the pericarp of sugar beet fruit, rather than the true seed. The presence of seed-borne inoculum should be considered when implementing integrated disease management strategies for CLS of sugar beet in the future.

scholarly journals Bacterial Endophytes As Indicators of Susceptibility To Cercospora Leaf Spot (CLS) Disease In Beta vulgaris L.

2021 ◽  
Author(s):  
Chiara Broccanello ◽  
Samathmika Ravi ◽  
Saptarathi Deb ◽  
Melvin Bolton ◽  
Gary Secor ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Leaf Spot ◽  
Durable Resistance ◽  
Pathogen Resistance ◽  
Cercospora Leaf Spot ◽  
Microbiome Composition ◽  
Hypervariable Regions ◽  
Increased Sensitivity ◽  
Untapped Resource

Abstract Background: The fungus Cercospora beticola causes Cercospora Leaf Spot (CLS) of sugar beet (Beta vulgaris L.). Despite the global importance of this disease, durable resistance to CLS has still not been obtained. Therefore, the development of tolerant hybrids is still a major goal for sugar beet breeding. Although recent studies have suggested that the leaf microbiome composition can offer useful predictors to assist plant breeders, this is an untapped resource in sugar beet breeding efforts. Methods: Using Ion GeneStudio S5 technology to sequence amplicons from seven 16S rRNA hypervariable regions, the most recurring endophytes discriminating CLS-symptomatic and symptomless sea beets (Beta vulgaris L.ssp. maritima) were identified. This allowed the design of taxon-specific primer pairs to quantify the abundance of the most representative endophytic species in large naturally occurring populations of sea beet and subsequently in sugar beet breeding genotypes under either CLS symptomless or infection stages using qPCR. Results: Among the screened bacterial genera, Methylobacterium and Mucilaginibacter were found to be significantly (p<0.05) more abundant in symptomatic sea beets with respect to symptomless. In cultivated sugar beet material under CLS infection, the comparison between resistant and susceptible genotypes confirmed that the susceptible genotypes hosted higher contents of the above-mentioned bacterial genera. Conclusions: These results suggest that the abundance of these species can be correlated with increased sensitivity to CLS disease. This evidence can further prompt novel protocols to assist plant breeding of sugar beet in the pursuit of improved pathogen resistance.

scholarly journals Glyphosate and fungicide effects on Cercospora leaf spot in four glyphosate-resistant sugar beet (Beta vulgaris) varieties

2013 ◽  
Vol 44 ◽  
pp. 38-43 ◽  
Author(s):  
William W. Kirk ◽  
Linda E. Hanson ◽  
Christy L. Sprague
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Leaf Spot ◽  
Cercospora Leaf Spot

Genetic and biochemical variations among sugar beet cultivars resistant to Cercospora leaf spot

2020 ◽  
Vol 109 ◽  
pp. 101455
Author(s):  
Bahaa E.S. Abd El-Fatah ◽  
Mohamed Hashem ◽  
Kamal A.M. Abo-Elyousr ◽  
Hadeel M.M. Khalil Bagy ◽  
Saad A.M. Alamri
Keyword(s):  
Sugar Beet ◽  
Leaf Spot ◽  
Cercospora Leaf Spot
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