Bio-hybrid photoconverter by covalent functionalization of the photosynthetic reaction center of Rhodobacter sphaeroides with fluorescein isothiocyanate

2015 ◽  
Vol 1722 ◽  
Author(s):  
Rocco Roberto Tangorra ◽  
Alessandra Antonucci ◽  
Francesco Milano ◽  
Simona la Gatta ◽  
Alessandra Operamolla ◽  
...  
Keyword(s):  
Hybrid Systems ◽  
Reaction Center ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Reaction Center ◽  
Fluorescein Isothiocyanate ◽  
Covalent Functionalization ◽  
Photosynthetic Proteins ◽  
Natural Photosynthesis

ABSTRACTOrganic biological hybrid systems, accessible by covalent functionalization of photosynthetic proteins with molecular antennas, represent a promising novelty to enhance natural photosynthesis. In this paper, we present the successful bioconjugation of a commercial fluorophore, fluorescein isothiocyanate (FITC), to the photosynthetic reaction center RC from the bacterium Rhodobacter sphaeroides strain R26. The resulting hybrid outperforms the pristine protein in hole-electron couple generation yield, exclusively at wavelengths where the fluorophore absorbs while the protein does not.

Bursting photosynthesis: designing ad-hoc fluorophores to complement the light harvesting capability of the photosynthetic reaction center

2014 ◽  
Vol 1689 ◽  
Author(s):  
Roberta Ragni ◽  
Omar Hassan Omar ◽  
Rocco Roberto Tangorra ◽  
Francesco Milano ◽  
Danilo Vona ◽  
...  
Keyword(s):  
Reaction Center ◽  
Ad Hoc ◽  
Light Harvesting ◽  
Photosynthetic Reaction Center ◽  
Covalent Functionalization ◽  
Photosynthetic Proteins ◽  
Powerful Approach ◽  
New Generation ◽  
Light Harvesting Antenna ◽  
Organic Fluorophore

ABSTRACTThe covalent functionalization of photosynthetic proteins with properly tailored organic molecular antennas represents a powerful approach to build a new generation of hybrid systems capable of exploiting solar energy. In this paper the strategy for the synthesis of the tailored aryleneethynylene organic fluorophore (AE) properly designed to act as light harvesting antenna is presented along with its successful bioconjugation to the photosynthetic reaction center RC from the bacterium Rhodobacter sphaeroides .

scholarly journals Effect of ultrasound on the function and structure of a membrane protein: The case study of photosynthetic Reaction Center from Rhodobacter sphaeroides

2017 ◽  
Vol 35 ◽  
pp. 103-111 ◽  
Author(s):  
Vincenzo De Leo ◽  
Lucia Catucci ◽  
A. Evelyn Di Mauro ◽  
Angela Agostiano ◽  
Livia Giotta ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Reaction Center ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Reaction Center

Structure of spheroidene in the photosynthetic reaction center from Y Rhodobacter sphaeroides

FEBS Letters ◽  
1989 ◽  
Vol 258 (1) ◽  
pp. 47-50 ◽  
Author(s):  
B. Arnoux ◽  
A. Ducruix ◽  
F. Reiss-Husson ◽  
M. Lutz ◽  
J. Norris ◽  
...  
Keyword(s):  
Reaction Center ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Reaction Center

Crystallographic Analyses of Site-Directed Mutants of the Photosynthetic Reaction Center from Rhodobacter sphaeroides

Biochemistry ◽  
1994 ◽  
Vol 33 (15) ◽  
pp. 4584-4593 ◽  
Author(s):  
Arthur J. Chirino ◽  
Erik J. Lous ◽  
Martina Huber ◽  
James P. Allen ◽  
Craig C. Schenck ◽  
...  
Keyword(s):  
Reaction Center ◽  
Rhodobacter Sphaeroides ◽  
Photosynthetic Reaction Center
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