Induction and characterization of oil palm (Elaeis guineensis Jacq.) pro-embryogenic masses

ABSTRACT Oil palm is one of the most economically valuable oil seed plants, but the expansion of plantations has been limited by availability of seedlings, as the conventional propagation is through seeds, which have low germination rates. One possible solution for the large-scale production is the use of somatic embryogenesis. The aim of this study was evaluate the effects auxins 2,4-D and picloram on the induction of pro-embryogenic masses in E.guineenesis hybrid leaf explants and characterize, regarding embryogenic characteristics, with cytochemical and ultrastructural analisys. Specifically, in vitro plantlets leaves fragments were inoculated in Y3 culture medium supplemented by 2.4-D or picloram at different concentrations (0.0, 1.0, 3.0, 6.0 and 9.0 mg l–1). After 90 days the presence/ absence of cell masses were evaluated. Both growth regulators efficiently induced cellular masses regardless of the concentrations applied. As the cell masses were not homogeneously formed, they were classified according to color and shape into four types: TYPE 1 - elongated and translucent, TYPE 2 – uneven and translucent, TYPE 3 - globular and beige TYPE 4 – globular and white. Based on the anatomical and ultrastructural features, TYPE 2, 3 and 4 cell masses were considered to have the highest embryogenic potential and therefore may be most suited to large-scale vegetative propagation of oil palm.

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INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ

Revista Árvore ◽

10.1590/1806-908820210000038 ◽

2021 ◽

Vol 45 ◽

Author(s):

Marlúcia Souza Pádua Vilela ◽

Jéssica de Castro e Andrade ◽

Raíssa Silveira Santos ◽

Vanessa Cristina Stein ◽

Patrick Callegari Magnani Santos Alves ◽

...

Keyword(s):

Oil Palm ◽

Large Scale ◽

Elaeis Guineensis ◽

Callus Formation ◽

Leaf Explants ◽

Dichlorophenoxyacetic Acid ◽

In Vitro Cultivation ◽

Low Concentrations ◽

2,4 Dichlorophenoxyacetic Acid

ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oil palm plants in low concentrations of auxins and to observe the maintenance of these characteristics during in vitro cultivation. Calluses were induced in 0.5 cm leaf explants in Y3 culture medium supplemented with Picloram (4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) or 2,4-D (2,4-dichlorophenoxyacetic acid), at concentrations of 0, 1, 3, 6, and 9 mg L-1. The callus with embryogenic appearance was subcultured and evaluated regarding maintenance of embryogenic characteristics by cytochemical analyses. The best treatment for induction of calluses was composed of 1mg.L-1 of Picloram, which led to 30% callus formation. The calluses were classified into4 types, based on color and morphology. The cells of calluses with nodular and beige appearance have embryogenic characteristics, and the embryogenic potential of the cell masses was maintained over the 20 months of cultivation. This differentiated adaptation to the protocol can allow the advance in the mass propagation of oil palm through tissue culture, indicating the importance of investigating the topics proposed by the research.

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DoE Analysis of Approaches for Hydrogel Microbeads’ Preparation by Millifluidic Methods

Micromachines ◽

10.3390/mi11111007 ◽

2020 ◽

Vol 11 (11) ◽

pp. 1007

Author(s):

Anna Nastruzzi ◽

Gabriele Pitingolo ◽

Giovanni Luca ◽

Claudio Nastruzzi

Keyword(s):

Mass Production ◽

Large Scale ◽

Single Cells ◽

Great Promise ◽

Scale Production ◽

Large Scale Production ◽

Different Types ◽

Cell Transplants

Hydrogel microbeads hold great promise for immune-protective cell transplants and in vitro studies. Millifluidic generation of hydrogel microbeads is a highly efficient and reproducible approach enabling a mass production. This paper illustrates the preparation and characterization of highly controlled and reproducible microbeads made by different types of hydrogel using millifluidic approaches. The optimization of the process was made by a design of experiments (DoE) approach. The microbeads’ large-scale production can be potentially used for single cells or clusters encapsulation.

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Large-scale production and physicochemical characterization of human immune interferon.

Infection and Immunity ◽

10.1128/iai.26.1.36-41.1979 ◽

1979 ◽

Vol 26 (1) ◽

pp. 36-41 ◽

Cited By ~ 108

Author(s):

M P Langford ◽

J A Georgiades ◽

G J Stanton ◽

F Dianzani ◽

H M Johnson

Keyword(s):

Large Scale ◽

Physicochemical Characterization ◽

Scale Production ◽

Immune Interferon ◽

Large Scale Production ◽

Human Immune

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Optimizing in vitro large scale production of giant reed (Arundo donax L.) by liquid medium culture

Biomass and Bioenergy ◽

10.1016/j.biombioe.2014.07.004 ◽

2014 ◽

Vol 69 ◽

pp. 21-27 ◽

Cited By ~ 13

Author(s):

Valeria Cavallaro ◽

Cristina Patanè ◽

Salvatore L. Cosentino ◽

Isabella Di Silvestro ◽

Venera Copani

Keyword(s):

Liquid Medium ◽

Large Scale ◽

Arundo Donax ◽

Giant Reed ◽

Scale Production ◽

Large Scale Production ◽

Medium Culture ◽

Arundo Donax L

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Large-scale production of polyoma middle T antigen by using genetically engineered tumors

Molecular and Cellular Biology ◽

10.1128/mcb.5.7.1795-1799.1985 ◽

1985 ◽

Vol 5 (7) ◽

pp. 1795-1799

Author(s):

D R Kaplan ◽

B Bockus ◽

T M Roberts ◽

J Bolen ◽

M Israel ◽

...

Keyword(s):

Nude Mice ◽

Large Scale ◽

T Antigen ◽

Genetically Engineered ◽

Scale Production ◽

Large Scale Production ◽

Metallothionein Promoter ◽

Polyoma Middle T Antigen ◽

Nih 3T3

A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 to 1,000 100-mm dishes of polyomavirus-infected cells. This middle T, partially purified by immunoaffinity chromatography, retained activity as measured by its ability to be phosphorylated in vitro. The combined approach of fusing strong promoters to genes of interest and utilizing nude mice to grow large quantities of cells expressing the gene provides a quick, inexpensive alternative to other expression systems.

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In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

Revista Árvore ◽

10.1590/1806-90882017000400014 ◽

2018 ◽

Vol 41 (4) ◽

Cited By ~ 1

Author(s):

Marlúcia Souza Pádua ◽

Raíssa Silveira Santos ◽

Luciano Vilela Paiva ◽

Vanessa Cristina Stein ◽

Luciano Coutinho Silva

Keyword(s):

Somatic Embryogenesis ◽

Growth Regulators ◽

Oil Palm ◽

Somatic Embryos ◽

Large Scale ◽

Elaeis Guineensis ◽

Germination Percentage ◽

Shoot Length ◽

Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

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Production of Phytotoxic Metabolite Using Biphasic Fermentation System from Strain C1136 of Lasiodiplodia pseudotheobromae, a Potential Bioherbicidal Agent

Notulae Scientia Biologicae ◽

10.15835/nsb9310082 ◽

2017 ◽

Vol 9 (3) ◽

pp. 371-377

Author(s):

Charles Oluwaseun ADETUNJI ◽

Julius Kola OLOKE ◽

Gandham PRASAD ◽

Moses ABALAKA ◽

Emenike Onyebum IROKANULO

Keyword(s):

Large Scale ◽

Wild Strain ◽

Fermentation Medium ◽

Scale Production ◽

Conventional Farming ◽

Large Scale Production ◽

Phytotoxic Metabolite ◽

Biphasic Fermentation

Formulation of effective and environmental friendly bioherbicides depends on the type of fermentation medium used for the production of phytotoxic metabolites. The effect of biomass, colony forming unit and the phytotoxic metabolite produced from the biphasic fermentation was carried out, while the phytotoxic metabolite was tested in vivo and in-vitro on Echinochola crus-galli and dicotyledonous Chromolaena odorata. The mutant strain of Lasiodiplodia pseudotheobromae C1136 (Lp90) produced the highest amount of conidia and the largest necrotic area on the two tested weeds when compared to its wild strain in the different biphasic media combinations. The study revealed that the biphasic system containing PDB + rice produced the highest bioherbicidal activities. Therefore, the phytotoxic metabolites from strain C1136 are suggested for large scale production of bioherbicides for the management of weeds in conventional farming to improve yield and enhance food security.

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