scholarly journals Comparison of Quick Lactose Intolerance Test in duodenal biopsies of dyspeptic patients with single nucleotide polymorphism LCT-13910C>T associated with primary hypolactasia/lactase-persistence

2013 ◽  
Vol 28 (suppl 1) ◽  
pp. 77-82 ◽  
Author(s):  
Rejane Mattar ◽  
Anibal Basile-Filho ◽  
Rafael Kemp ◽  
José Sebastião dos Santos
Keyword(s):  
Lactose Intolerance ◽  
Genetic Test ◽  
Lactase Persistence ◽  
Upper Gastrointestinal Endoscopy ◽  
Polymorphism Analysis ◽  
Kappa Index ◽  
Quick Test ◽  
Pcr Product ◽  
Highly Sensitive ◽  
Duodenal Biopsies

PURPOSE: To analyze the usefulness of Quick Lactose Intolerance Test in relation to the genetic test based on LCT-13910C>T genotypes, previously validated for clinical practice, for primary hypolactasia/lactase-persistence diagnosis. METHODS: Thirty-two dyspeptic patients that underwent upper gastrointestinal endoscopy entered the study. Two postbulbar duodenal biopsies were taken for the Quick test, and gastric antral biopsy for DNA extraction and LCT-13910C>T polymorphism analysis. DNA was also extracted from biopsies after being used in the Quick Test that was kept frozen until extraction. RESULTS: Nine patients with lactase-persistence genotype (LCT-13910CT or LCT-13910TT) had normolactasia, eleven patients with hypolactasia genotype (LCT-13910CC) had severe hypolactasia, and among twelve with mild hypolactasia, except for one that had LCT-13910CT genotype, all the others had hypolactasia genotype. The agreement between genetic test and quick test was high (p<0.0001; Kappa Index 0.92). Most of the patients that reported symptoms with lactose-containing food ingestion had severe hypolactasia (p<0.05). Amplification with good quality PCR product was also obtained with DNA extracted from biopsies previously used in the Quick Test; thus, for the future studies antral gastric biopsies for genetic test would be unnecessary. CONCLUSION: Quick test is highly sensitive and specific for hypolactasia diagnosis and indicated those patients with symptoms of lactose intolerance.

S2067 A Comparison Between Lactose Breath Test and a Quick Test On Duodenal Biopsies for Lactase Deficiency, in Patients with a Self Reported Lactose Intolerance

2009 ◽  
Vol 136 (5) ◽  
pp. A-323
Author(s):  
Franzè Jolanda ◽  
Andrea Parodi ◽  
Edoardo Savarino ◽  
Ester Morana ◽  
Anna Bertelè ◽  
...  
Keyword(s):  
Breath Test ◽  
Lactose Intolerance ◽  
Lactase Deficiency ◽  
Quick Test ◽  
Duodenal Biopsies

A Comparison Between Lactose Breath Test and Quick Test on Duodenal Biopsies for Diagnosing Lactase Deficiency in Patients With Self-reported Lactose Intolerance

2013 ◽  
Vol 47 (2) ◽  
pp. 148-152 ◽  
Author(s):  
Manuele Furnari ◽  
Daria Bonfanti ◽  
Andrea Parodi ◽  
Jolanda Franzè ◽  
Edoardo Savarino ◽  
...  
Keyword(s):  
Breath Test ◽  
Lactose Intolerance ◽  
Lactase Deficiency ◽  
Quick Test ◽  
Duodenal Biopsies

scholarly journals Prevalence of lactase persistence and the performance of a non-invasive genetic test in adult Sardinian patients

2010 ◽  
Vol 5 (1) ◽  
pp. e1-e5 ◽  
Author(s):  
Domenica A. Obinu ◽  
Nabil S. Enattah ◽  
Antonietta Pedroni ◽  
Leena Peltonen ◽  
Luca L. Cavalli-Sforza ◽  
...  
Keyword(s):  
Genetic Test ◽  
Lactase Persistence ◽  
Non Invasive

A Study on Genetic Test of Lactase Persistence in Relation to Milk Consumption in Regional Groups of India

2012 ◽  
Vol 16 (12) ◽  
pp. 1413-1418 ◽  
Author(s):  
Shruti V. Baadkar ◽  
Manjari S. Mukherjee ◽  
Smita S. Lele
Keyword(s):  
Genetic Test ◽  
Lactase Persistence ◽  
Milk Consumption

scholarly journals Comparative evaluation of published cytomegalovirus primers for rapid real-time PCR: which are the most sensitive?

2009 ◽  
Vol 58 (7) ◽  
pp. 878-883 ◽  
Author(s):  
Wafa Habbal ◽  
Fawza Monem ◽  
Barbara C. Gärtner
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Analytical Sensitivity ◽  
Pcr Product ◽  
Highly Sensitive ◽  
Primer Sets ◽  
Analytical Sensitivities ◽  
Strain Ad169

Standardization of human cytomegalovirus (CMV) PCR is highly recommended. As primer design is essential for PCR sensitivity, this study evaluated all published CMV primer pairs to identify the most sensitive for single-round real-time PCR. PubMed (1993–2004) was searched for original papers aimed at CMV PCR. Fifty-seven papers were identified revealing 82 different primer pairs. Of these, 17 primer sets were selected for empirical study, as they were either used in real-time PCR or were evaluated comparatively by conventional PCR. After optimizing the PCR conditions, these primer sets were evaluated by real-time PCR using a SYBR Green format. Analytical sensitivities were assessed by testing the reference standard CMV strain AD169. A blast search was performed to identify mismatches with published sequences. Additionally, 60 clinical samples were tested with the three primer sets showing highest analytical sensitivity and the best match to all CMV strains. Three primer sets located in the glycoprotein B (UL55) gene region were found to be the most sensitive using strain AD169. However, two of these showed a considerable number of mismatches with clinical isolates in a blast search. Instead, two other pairs from the lower matrix phosphoprotein (UL83) gene and DNA polymerase (UL54) gene showed reasonable sensitivity and no mismatches with clinical isolates. These three pairs were further tested with clinical samples, which indicated that the two primer sets from UL55 and UL54 were the most sensitive. Interestingly, the analytical sensitivity of the PCR was inversely correlated with the size of the PCR product. In conclusion, these two primer pairs are recommended for a standardized, highly sensitive, real-time PCR.

scholarly journals Use of Multiplex PCR and PCR Restriction Enzyme Analysis for Detection and Exploration of the Variability in the Free-Living Amoeba Naegleria in the Environment

2002 ◽  
Vol 68 (4) ◽  
pp. 2061-2065 ◽  
Author(s):  
Michel Pélandakis ◽  
Pierre Pernin
Keyword(s):  
Multiplex Pcr ◽  
Fragment Length Polymorphism ◽  
Internal Transcribed Spacers ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Polymorphism Analysis ◽  
Free Living ◽  
Pcr Product ◽  
Free Living Amoeba ◽  
Restriction Fragment Length

ABSTRACT A multiplex PCR was developed to simultaneously detect Naegleria fowleri and other Naegleria species in the environment. Multiplex PCR was also capable of identifying N. fowleri isolates with internal transcribed spacers of different sizes. In addition, restriction fragment length polymorphism analysis of the PCR product distinguished the main thermophilic Naegleria species from the sampling sites.

scholarly journals Diagnosis of adult-type hypolactasia/lactase persistence: genotyping of single nucleotide polymorphism (SNP C/T-13910) is not consistent with breath test in Colombian Caribbean population

2012 ◽  
Vol 49 (1) ◽  
pp. 5-8 ◽  
Author(s):  
Evelyn Mendoza Torres ◽  
Lourdes Luz Varela Prieto ◽  
José Luis Villarreal Camacho ◽  
Daniel Antonio Villanueva Torregroza
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Breath Test ◽  
Lactase Persistence ◽  
Kappa Index ◽  
Nucleotide Polymorphism ◽  
Hydrogen Breath Test ◽  
Adult Type ◽  
Single Nucleotide ◽  
Moderate Agreement ◽  
Caribbean Population

CONTEXT: Genotyping of single nucleotide polymorphism (SNP C/T-13910) located upstream of the lactase gene is used to determine adult-type hypolactasia/lactase persistence in North-European Caucasian subjects. The applicability of this polymorphism has been studied by comparing it with the standard diagnostic methods in different populations. OBJECTIVE: To compare the lactose hydrogen breath test with the genetic test in a sample of the Colombian Caribbean population. METHODS: Lactose hydrogen breath test and genotyping of SNP C/T-13910 were applied to 128 healthy individuals (mean age 35 ± 1). A positive lactose hydrogen breath test was indicative of hypolactasia. Genotyping was done using polymerase chain reaction/restriction fragment length polymorphism. The kappa index was used to establish agreement between the two methods. RESULTS: Seventy-six subjects (59%) were lactose-maldigesters (hypolactasia) and 52 subjects (41%) were lactose-digesters (lactase persistence). The frequencies of the CC, CT and TT genotypes were 80%, 20% and 0%, respectively. Genotyping had 97% sensitivity and 46% specificity. The kappa index = 0.473 indicates moderate agreement between the genotyping of SNP C/T-13910 and the lactose hydrogen breath test. CONCLUSION: The moderate agreement indicates that the genotyping of the SNP C/T-13910 is not applicable to determine adult-type hypolactasia/lactase persistence in the population participating in this study.

scholarly journals Congruency of Genetic Predisposition to Lactase Persistence and Lactose Breath Test

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1383 ◽  
Author(s):  
Enza Coluccia ◽  
Patrizia Iardino ◽  
Diego Pappalardo ◽  
Anna Lisa Brigida ◽  
Vincenzo Formicola ◽  
...  
Keyword(s):  
Breath Test ◽  
Lactose Intolerance ◽  
Genetic Test ◽  
Gastrointestinal Symptoms ◽  
Diagnostic Procedures ◽  
Clinical Syndrome ◽  
Clinical Use ◽  
Genotype C ◽  
Lactase Gene ◽  
Genotype A

The physiological decline of lactase production in adulthood, in some individuals, is responsible for the so-called “Lactose Intolerance.” This clinical syndrome presents with gastrointestinal and non-gastrointestinal symptoms following the consumption of dairy containing food. Lactose intolerance can be evaluated by means of the Lactose Breath Test (phenotype) and/or genetic evaluation of lactase-gene polymorphism (genotype). A comparison of the two tests was carried out in a large number of symptomatic adult subjects, which are selected and not representative of the general population. Congruency was as high as 88.6%. Among lactase non-persistent (genotype C/C), 14 subjects showed a negative Lactose Breath Test (LBT), possibly due to young age. Among lactase-persistent (genotype C/T), four subjects showed a positive LBT, which helps to diagnose secondary lactose intolerance. Symptoms, both gastrointestinal and extra-gastrointestinal, were reported by 90% of patients during the breath test. Clinical use of both tests in the same patients could be taken into consideration as a sharp diagnostic tool. We suggest considering the use of the genetic test after LBT administration, when secondary hypolactasia is suspected, for completion of diagnostic procedures.

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