S2067 A Comparison Between Lactose Breath Test and a Quick Test On Duodenal Biopsies for Lactase Deficiency, in Patients with a Self Reported Lactose Intolerance

PURPOSE: To analyze the usefulness of Quick Lactose Intolerance Test in relation to the genetic test based on LCT-13910C>T genotypes, previously validated for clinical practice, for primary hypolactasia/lactase-persistence diagnosis. METHODS: Thirty-two dyspeptic patients that underwent upper gastrointestinal endoscopy entered the study. Two postbulbar duodenal biopsies were taken for the Quick test, and gastric antral biopsy for DNA extraction and LCT-13910C>T polymorphism analysis. DNA was also extracted from biopsies after being used in the Quick Test that was kept frozen until extraction. RESULTS: Nine patients with lactase-persistence genotype (LCT-13910CT or LCT-13910TT) had normolactasia, eleven patients with hypolactasia genotype (LCT-13910CC) had severe hypolactasia, and among twelve with mild hypolactasia, except for one that had LCT-13910CT genotype, all the others had hypolactasia genotype. The agreement between genetic test and quick test was high (p<0.0001; Kappa Index 0.92). Most of the patients that reported symptoms with lactose-containing food ingestion had severe hypolactasia (p<0.05). Amplification with good quality PCR product was also obtained with DNA extracted from biopsies previously used in the Quick Test; thus, for the future studies antral gastric biopsies for genetic test would be unnecessary. CONCLUSION: Quick test is highly sensitive and specific for hypolactasia diagnosis and indicated those patients with symptoms of lactose intolerance.

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A BOY WITH SEVERE INFANTILE GASTROGEN LACTOSE INTOLERANCE AND ACQUIRED LACTASE DEFICIENCY

Acta Paediatrica ◽

10.1111/j.1651-2227.1979.tb18451.x ◽

1979 ◽

Vol 68 (5) ◽

pp. 751-758 ◽

Cited By ~ 4

Author(s):

N. O. BERG ◽

A. DAHLQVIST ◽

T. LINDBERG

Keyword(s):

Lactose Intolerance ◽

Lactase Deficiency

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Comparison of an increased waist circumference with a positive hydrogen breath test as a clinical predictor of lactose intolerance

Archivos Argentinos de Pediatria ◽

10.5546/aap.2017.eng.148 ◽

2017 ◽

Vol 115 (2) ◽

Keyword(s):

Waist Circumference ◽

Breath Test ◽

Lactose Intolerance ◽

Hydrogen Breath Test ◽

Clinical Predictor

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Concordance between Lactose Quick Test, hydrogen-methane breath test and genotyping for the diagnosis of lactose malabsorption in children

Neurogastroenterology & Motility ◽

10.1111/nmo.13271 ◽

2017 ◽

Vol 30 (5) ◽

pp. e13271 ◽

Cited By ~ 2

Author(s):

C. Rojo ◽

F. Jaime ◽

L. Azócar ◽

C. Hernández ◽

A. Villagrán ◽

...

Keyword(s):

Breath Test ◽

Lactose Malabsorption ◽

Quick Test

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Lactose Intolerance and Bone Health: The Challenge of Ensuring Adequate Calcium Intake

Nutrients ◽

10.3390/nu11040718 ◽

2019 ◽

Vol 11 (4) ◽

pp. 718 ◽

Cited By ~ 16

Author(s):

Joanna Hodges ◽

Sisi Cao ◽

Dennis Cladis ◽

Connie Weaver

Keyword(s):

Bone Health ◽

Calcium Intake ◽

Lactose Intolerance ◽

Calcium Absorption ◽

Fragility Fractures ◽

Food Sources ◽

Nutritional Deficiencies ◽

Free Calcium ◽

Lactase Deficiency ◽

Dairy Consumption

Calcium is an important nutrient with impact upon many biological systems, most notably bone. Ensuring adequate calcium intake throughout the lifespan is essential to building and maintaining bone. Lactose intolerance may predispose individuals to low calcium intake as the number of lactose-free, calcium-rich food sources is limited. In this review, we summarize data from human and animal studies on the influence of lactose and lactase deficiency on calcium absorption and bone health. Based on the available evidence, neither dietary lactose nor lactase deficiency have a significant impact on calcium absorption in adult humans. However, lactose intolerance may lead to reduced bone density and fragility fractures when accompanied by decreased intake or avoidance of dairy. Recently published human trials and meta-analyses suggest a weak but significant association between dairy consumption and bone health, particularly in children. Given the availability of simple dietary approaches to building lactose tolerance and the nutritional deficiencies associated with dairy avoidance, multiple public health organizations recommend that all individuals—including those that are lactose intolerant—consume three servings of dairy per day to ensure adequate nutrient intakes and optimal bone health.

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Human Health Effects of Lactose Consumption as a Food and Drug Ingredient

Current Pharmaceutical Design ◽

10.2174/1381612826666200212114843 ◽

2020 ◽

Vol 26 (16) ◽

pp. 1778-1789 ◽

Cited By ~ 1

Author(s):

Rafael G. Seoane ◽

Verónica Garcia-Recio ◽

Manuel Garrosa ◽

María Á. Rojo ◽

Pilar Jiménez ◽

...

Keyword(s):

Premature Infants ◽

Dairy Products ◽

Lactose Intolerance ◽

Pharmaceutical Formulations ◽

Reducing Sugar ◽

The Other ◽

Lactase Deficiency ◽

Adult Type ◽

Drug Ingredient ◽

Human Health Effects

: Lactose is a reducing sugar consisting of galactose and glucose, linked by a β (1→4) glycosidic bond, considered as an antioxidant due to its α-hydroxycarbonyl group. Lactose is widely ingested through the milk and other unfermented dairy products and is considered to be one of the primary foods. On the other hand, lactose is also considered as one of the most widely used excipients for the development of pharmaceutical formulations. In this sense, lactose has been related to numerous drug-excipient or drug-food pharmacokinetic interactions. : Intolerance, maldigestion and malabsorption of carbohydrates are common disorders in clinical practice, with lactose-intolerance being the most frequently diagnosed, afflicting 10% of the world’s population. Four clinical subtypes of lactose intolerance may be distinguished, namely lactase deficiency in premature infants, congenital lactase deficiency, adult-type hypolactasia and secondary lactase intolerance. An overview of the main uses of lactose in human nutrition and in the pharmaceutical industry and the problems derived from this circumstance are described in this review.

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Transient Lactose Intolerance in Premature Infants

PEDIATRICS ◽

10.1542/peds.48.5.816 ◽

1971 ◽

Vol 48 (5) ◽

pp. 816-821

Author(s):

Felix Abdo-Bassols ◽

Fima Lifshitz ◽

Ernesto Diaz Del Castillo ◽

Virginia Martinez-Garza

Keyword(s):

Premature Infants ◽

Lactose Intolerance ◽

Congenital Defect ◽

The Other ◽

Milk Formula ◽

Lactase Deficiency ◽

Diminished Capacity ◽

Other Hand

Healthy premature infants have a relative lactase deficiency resulting in a diminished capacity to digest and absorb lactose.1-6 However, milk formula feedings do not result in diarrhea in these babies. On the other hand, infants with significant intestinal lactase deficiency have diarrhea that characteristically improves after elimination of this disaccharide from the diet.7-8 In some of these patients, the illness began at birth and was classified as a congenital defect.9-11 The purpose of this report is to describe two premature infants who presented transient lactose intolerance since birth. The parents of both patients and two siblings of one were also intolerant to this disaccharide.

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