scholarly journals Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor

2004 ◽  
Vol 47 (5) ◽  
pp. 821-826 ◽  
Author(s):  
Ana Lúcia Figueiredo Porto ◽  
Leonie Asfora Sarubbo ◽  
Keila Aparecida Moreira ◽  
Homero José Farias de Melo ◽  
José Luís Lima-Filho ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Phase Velocity ◽  
Crude Extract ◽  
Separation Efficiency ◽  
Dispersed Phase ◽  
Phase System ◽  
Transfer Coefficients ◽  
Rotating Disc ◽  
Two Phase ◽  
Rotating Disc Contactor

A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The best recovery of enzyme activity was 236%, with a purification factor of 34 in flow rates of 1 mL/min for dispersed phase.

Performance of a perforated rotating disc contactor in the continuous extraction of a protein using the PEG–cashew-nut tree gum aqueous two-phase system

2003 ◽  
Vol 16 (3) ◽  
pp. 221-227 ◽  
Author(s):  
Leonie A Sarubbo ◽  
Luciana A Oliveira ◽  
Ana Lúcia F Porto ◽  
José Luiz Lima-Filho ◽  
Galba Maria Campos-takaki ◽  
...  
Keyword(s):  
Phase System ◽  
Rotating Disc ◽  
Continuous Extraction ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Cashew Nut ◽  
Rotating Disc Contactor

Hydrodynamics and mass transfer in aqueous two-phase protein extraction using a continuous perforated rotating disc contactor

2000 ◽  
Vol 22 (3) ◽  
pp. 0215-0218 ◽  
Author(s):  
A. L. F. Porto ◽  
L. A. Sarubbo ◽  
J. L. Lima-Filho ◽  
M. R. Aires-Barros ◽  
J. M. S. Cabral ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Protein Extraction ◽  
Rotating Disc ◽  
Two Phase ◽  
Rotating Disc Contactor ◽  
Phase Protein

Lyon-Type Integral Forms of Wall Friction, Heat- and Mass Transfer Closure Relationships for Non-Equilibrium Two-Phase Flows: Generalization for Annular and Rod Cluster Geometries

2013 ◽  
Author(s):  
Yuri Kornienko
Keyword(s):  
Boundary Layer ◽  
Mass Transfer ◽  
Heat And Mass Transfer ◽  
Constitutive Relations ◽  
Wall Friction ◽  
Transfer Coefficients ◽  
Two Phase ◽  
Friction Heat ◽  
Integral Forms ◽  
Substance Transfer

The main goal of this paper is to describe new approach to constructing generalized closure relationships for pipe, annular and sub-channel transfer coefficients for wall friction, heat and mass transfer. The novelty of this approach is that it takes into account not only axial and transversal parameter distributions, but also an azimuthal substance transfer effects. These constitutive relations, which are primordial in the description of single- and two-phase one-dimensional (1D) flow models, can be derived from the initial 3D drift flux formulation. The approach is based on the Reynolds flow, boundary layer, and substance transfer generalized coefficient concepts. Another aim is to illustrate the validity of the “conformity principle” for the limiting cases. The method proposed in this paper is founded on the similarity theory, boundary layer model, and a phenomenological description of the regularity of the substance transfer (momentum, heat, and mass) as well as on an adequate simulation of the flow structures. With the proposed generalized approach it becomes possible to develop an integrated in form and semi-empirical in maintenance structure analytical relationships for wall friction, heat and mass transfer coefficients.

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