scholarly journals Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by multiple-locus variable number-tandem repeat analysis

2015 ◽  
Vol 46 (2) ◽  
pp. 557-564 ◽  
Author(s):  
Andrea Gioffré ◽  
Magnolia Correa Muñoz ◽  
María F. Alvarado Pinedo ◽  
Roberto Vaca ◽  
Claudia Morsella ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Tandem Repeat ◽  
Molecular Typing ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Mycobacterium Avium Subsp ◽  
Multiple Locus ◽  
Repeat Analysis ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals Evidence of in utero infection by Mycobacterium avium subsp. paratuberculosis using Multiple-Locus Variable-number tandem-repeat Analysis:

2020 ◽  
Vol 57 (1) ◽  
pp. e161653
Author(s):  
Brenda Vasini Rosell ◽  
Bernardo Lagleyze ◽  
Claudia Morsella ◽  
Laura Mendez ◽  
Florencia Bresky ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Mycobacterium Avium Subsp ◽  
Multiple Locus ◽  
Repeat Analysis ◽  
Acid Fast Bacilli ◽  
Specific Medium ◽  
Mycobacterium Avium Subsp Paratuberculosis

A pregnant heifer with an advanced clinical stage of paratuberculosis was reported in a herd in Argentina. Thus, the animal was euthanized and samples of organs of the cow and its fetus was taken and cultured for bacteriology in specific medium. Tissues were analyzed by histopathology (hematoxylin-eosin and Ziehl-Neelsen staining). Histopathological analysis of the cow’s samples revealed the presence of lesions consistent with paratuberculosis, and Ziehl-Neelsen staining revealed the presence of acid-fast bacilli, whereas the fetal tissues showed absence of lesions but the presence of acid-fast bacilli by Ziehl-Neelsen staining. After growing in specific medium, colonies in tissues from both cow and fetus were positive for IS900-PCR, confirming the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Finally, the isolates were typed by Multiple-Locus Variable-number tandem-repeat Analysis (MLVA), which confirmedthe epidemiological link between them. This study is the first in Argentina to report the detection of MAP that shares an identical MLVA type in a pregnant cow and its fetus. The results of this study are consistent with previous reports and highlight the intra-uterine transmission of MAP as an important source of infection within herds.

Molecular typing of Francisella tularensis using multiple-locus variable-number tandem-repeat analysis

2014 ◽  
Vol 29 (1) ◽  
pp. 8-15 ◽  
Author(s):  
V. S. Timofeev ◽  
T. Yu. Kudryavtseva ◽  
A. N. Mokrievich ◽  
V. M. Pavlov ◽  
I. A. Dyatlov
Keyword(s):  
Francisella Tularensis ◽  
Tandem Repeat ◽  
Molecular Typing ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis

scholarly journals Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus

2010 ◽  
Vol 10 (1) ◽  
pp. 315 ◽  
Author(s):  
Simon Thierry ◽  
Dongying Wang ◽  
Pascal Arne ◽  
Manju Deville ◽  
Barbara De Bruin ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Tandem Repeat ◽  
Molecular Typing ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Multiple Locus ◽  
Repeat Analysis
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