New polymorphisms within the variable number tandem repeat (VNTR) 7 locus of Mycobacterium avium subsp. paratuberculosis

2016 ◽  
Vol 30 (3) ◽  
pp. 132-137 ◽  
Author(s):  
Ahmad Fawzy ◽  
Michael Zschöck ◽  
Christa Ewers ◽  
Tobias Eisenberg
Keyword(s):  
Mycobacterium Avium ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Mycobacterium Avium Subsp ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals Evidence of in utero infection by Mycobacterium avium subsp. paratuberculosis using Multiple-Locus Variable-number tandem-repeat Analysis:

2020 ◽  
Vol 57 (1) ◽  
pp. e161653
Author(s):  
Brenda Vasini Rosell ◽  
Bernardo Lagleyze ◽  
Claudia Morsella ◽  
Laura Mendez ◽  
Florencia Bresky ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Mycobacterium Avium Subsp ◽  
Multiple Locus ◽  
Repeat Analysis ◽  
Acid Fast Bacilli ◽  
Specific Medium ◽  
Mycobacterium Avium Subsp Paratuberculosis

A pregnant heifer with an advanced clinical stage of paratuberculosis was reported in a herd in Argentina. Thus, the animal was euthanized and samples of organs of the cow and its fetus was taken and cultured for bacteriology in specific medium. Tissues were analyzed by histopathology (hematoxylin-eosin and Ziehl-Neelsen staining). Histopathological analysis of the cow’s samples revealed the presence of lesions consistent with paratuberculosis, and Ziehl-Neelsen staining revealed the presence of acid-fast bacilli, whereas the fetal tissues showed absence of lesions but the presence of acid-fast bacilli by Ziehl-Neelsen staining. After growing in specific medium, colonies in tissues from both cow and fetus were positive for IS900-PCR, confirming the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Finally, the isolates were typed by Multiple-Locus Variable-number tandem-repeat Analysis (MLVA), which confirmedthe epidemiological link between them. This study is the first in Argentina to report the detection of MAP that shares an identical MLVA type in a pregnant cow and its fetus. The results of this study are consistent with previous reports and highlight the intra-uterine transmission of MAP as an important source of infection within herds.

scholarly journals Mycobacterioses in dogs and cats from Buenos Aires, Argentina

2017 ◽  
Vol 29 (5) ◽  
pp. 729-732 ◽  
Author(s):  
Soledad Barandiaran ◽  
Marcela Martínez Vivot ◽  
Elvira Falzoni ◽  
María J. Marfil ◽  
Gabriela Pérez Tort ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Tandem Repeat ◽  
Potential Risk ◽  
Buenos Aires ◽  
Variable Number Tandem Repeat ◽  
Clinical Signs ◽  
Variable Number ◽  
Molecular Testing ◽  
Full Characterization

Mycobacterioses can produce nonspecific clinical signs in dogs and cats that make diagnosis difficult. Furthermore, the full characterization of mycobacterial agents is not always possible or practical. We characterized mycobacteria detected through cytology in 12 dogs and 7 cats with generalized clinical signs from the province of Buenos Aires in Argentina. In dogs, molecular testing confirmed the presence of Mycobacterium avium subsp. hominissuis (MAH) in 8 cases and M. fortuitum in 1 case. All dogs were Miniature Schnauzers, suggesting that this breed may be more susceptible to M. avium than other dog breeds. The cat isolates were 2 M. bovis, 1 M. fortuitum, and 1 MAH. Mycobacterial interspersed repetitive unit–variable-number tandem repeat patterns suggested possible links with cattle, swine, and humans studied previously in Argentina. The results show that pets may act as susceptible hosts with the potential risk of transmitting the infection to humans and other animals.

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