Exemplar Abstract for Methylobacterium dichloromethanicum chloromethanicum (McDonald et al. 2001) Hördt et al. 2020 and Methylobacterium chloromethanicum McDonald et al. 2001 emend. Hördt et al. 2020.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Methylobacterium Dichloromethanicum

The Aeration-Dependent Effect of Vitamin B12on DNA Biosynthesis in Methylobacterium dichloromethanicum

Microbiology ◽  
2004 ◽  
Vol 73 (2) ◽  
pp. 134-138 ◽  
Author(s):  
I. V. Danilova ◽  
N. V. Doronina ◽  
Yu. A. Trotsenko ◽  
A. I. Netrusov ◽  
E. P. Ryzhkova (Iordan)
Keyword(s):  
Dependent Effect ◽  
Dna Biosynthesis ◽  
Methylobacterium Dichloromethanicum

Nomenclature Abstract for Methylobacterium dichloromethanicum Doronina et al. 2000.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity
Keyword(s):  
Methylobacterium Dichloromethanicum

scholarly journals DNA Polymerase I Is Essential for Growth ofMethylobacterium dichloromethanicum DM4 with Dichloromethane

2000 ◽  
Vol 182 (19) ◽  
pp. 5433-5439 ◽  
Author(s):  
Martin F. Kayser ◽  
Michael T. Stumpp ◽  
Stéphane Vuilleumier
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Uv Light ◽  
Polymerase Activity ◽  
Dna Polymerase I ◽  
Gene Encoding ◽  
Cell Extracts ◽  
Dichloromethane Dehalogenase ◽  
Polymerase I ◽  
Methylobacterium Dichloromethanicum

ABSTRACT Methylobacterium dichloromethanicum DM4 grows with dichloromethane as the unique carbon and energy source by virtue of a single enzyme, dichloromethane dehalogenase–glutathioneS-transferase. A mutant of the dichloromethane-degrading strain M. dichloromethanicum DM4, strain DM4-1445, was obtained by mini-Tn5 transposon mutagenesis that was no longer able to grow with dichloromethane. Dichloromethane dehalogenase activity in this mutant was comparable to that of the wild-type strain. The site of mini-Tn5 insertion in this mutant was located in the polA gene encoding DNA polymerase I, an enzyme with a well-known role in DNA repair. DNA polymerase activity was not detected in cell extracts of the polA mutant. Conjugation of a plasmid containing the intact DNA polymerase I gene into thepolA mutant restored growth with dichloromethane, indicating that the polA gene defect was responsible for the observed lack of growth of this mutant with dichloromethane. Viability of the DM4-1445 mutant was strongly reduced upon exposure to both UV light and dichloromethane. The polA′-lacZtranscriptional fusion resulting from mini-Tn5 insertion was constitutively expressed at high levels and induced about twofold after addition of 10 mM dichloromethane. Taken together, these data indicate that DNA polymerase I is essential for growth of M. dichloromethanicum DM4 with dichloromethane and further suggest an important role of the DNA repair machinery in the degradation of halogenated, DNA-alkylating compounds by bacteria.

Functionality of Metdi5511gene in Methylobacterium dichloromethanicum DM4

2017 ◽  
Vol 53 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Yu. E. Firsova ◽  
M. L. Torgonskaya ◽  
Yu. A. Trotsenko
Keyword(s):  
Methylobacterium Dichloromethanicum

Functional activity of the modA 2 gene in Methylobacterium dichloromethanicum DM4

Microbiology ◽  
2014 ◽  
Vol 83 (3) ◽  
pp. 205-210
Author(s):  
Y. E. Firsova ◽  
Y. A. Trotsenko
Keyword(s):  
Functional Activity ◽  
Methylobacterium Dichloromethanicum

Functionality of the xoxF gene in Methylobacterium dichloromethanicum DM4

Microbiology ◽  
2015 ◽  
Vol 84 (6) ◽  
pp. 796-803 ◽  
Author(s):  
Yu. E. Firsova ◽  
M. L. Torgonskaya ◽  
Yu. A. Trotsenko
Keyword(s):  
Methylobacterium Dichloromethanicum
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