scholarly journals Anaylsis of the SARS-CoV and IAV-dependent transcriptomes in human airway epithelial cell cultures

SPP Datasets ◽  
2020 ◽  
Author(s):  
R Baric
Keyword(s):  
Epithelial Cell ◽  
Cell Cultures ◽  
Airway Epithelial Cell ◽  
Airway Epithelial ◽  
Epithelial Cell Cultures ◽  
Human Airway ◽  
Human Airway Epithelial Cell

Well-Differentiated Human Airway Epithelial Cell Cultures

2004 ◽  
pp. 183-206 ◽  
Author(s):  
M. Leslie Fulcher ◽  
Sherif Gabriel ◽  
Kimberlie A. Burns ◽  
James R. Yankaskas ◽  
Scott H. Randell
Keyword(s):  
Epithelial Cell ◽  
Cell Cultures ◽  
Airway Epithelial Cell ◽  
Airway Epithelial ◽  
Epithelial Cell Cultures ◽  
Human Airway ◽  
Human Airway Epithelial Cell ◽  
Well Differentiated

Prostaglandin E2-induced interleukin-6 release by a human airway epithelial cell line

2001 ◽  
Vol 280 (1) ◽  
pp. L127-L133 ◽  
Author(s):  
S. Tavakoli ◽  
M. J. Cowan ◽  
T. Benfield ◽  
C. Logun ◽  
J. H. Shelhamer
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Airway Epithelial Cell ◽  
Epithelial Cell Line ◽  
Receptor Subtype ◽  
Receptor Subtypes ◽  
Airway Epithelial ◽  
Human Airway ◽  
Human Airway Epithelial Cell ◽  
Cell Release

Human airway epithelial cell release of interleukin (IL)-6 in response to lipid mediators was studied in an airway cell line (BEAS-2B). Prostaglandin (PG) E2(10−7M) treatment caused an increase in IL-6 release at 2, 4, 8, and 24 h. IL-6 release into the culture medium at 24 h was 3,396 ± 306 vs. 1,051 ± 154 pg/ml (PGE2-treated cells vs. control cells). PGE2(10−7to 10−10M) induced a dose-related increase in IL-6 release at 24 h. PGF2α(10−6M) treatment caused a similar effect to that of PGE2(10−7M). PGE2analogs with relative selectivity for PGE2receptor subtypes were studied. Sulprostone, a selective agonist for the EP-3 receptor subtype had no effect on IL-6 release. 11-Deoxy-16,16-dimethyl-PGE2, an EP-2/4 agonist, and 17-phenyl trinor PGE2, an agonist selective for the EP-1 > EP-3 receptor subtype (10−6to 10−8M), caused dose-dependent increases in IL-6 release. 8-Bromo-cAMP treatment resulted in dose-related increases in IL-6 release. RT-PCR of BEAS-2B cell mRNA demonstrated mRNA for EP-1, EP-2, and EP-4 receptors. After PGE2treatment, increases in IL-6 mRNA were noted at 4 and 18 h. Therefore, PGE2increases airway epithelial cell IL-6 production and release.

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