Liposome-mediated macrophage depletion: an experimental approach to study the role of testicular macrophages in the rat

1993 ◽  
Vol 136 (3) ◽  
pp. 407-NP ◽  
Author(s):  
A. Bergh ◽  
J.-E. Damber ◽  
N. van Rooijen
Keyword(s):  
Leydig Cell ◽  
Cell Function ◽  
Physiological Role ◽  
Adult Rats ◽  
Testosterone Secretion ◽  
Testicular Weight ◽  
The Right ◽  
Leydig Cell Function ◽  
Testicular Macrophages

ABSTRACT Liposome-entrapped dichloromethylene diphosphonate was injected locally into the right testes of adult rats. This treatment, which has been found to deplete resident macrophages in some other organs, reduced the number of testicular macrophages by at least 90%. Testicular weight and seminiferous tubule morphology were unaffected by liposome treatment. Leydig cell testosterone secretion gradually declined in the macrophage-depleted testes, and there was a compensatory increase in Leydig cell size and testosterone secretion in the contralateral saline-injected testes. These observations suggest that macrophages influence Leydig cell function locally. It is concluded that liposome-mediated depletion of testicular macrophages may serve as an experimental model with which to study the physiological role of these cells. Journal of Endocrinology (1993) 136, 407–413

scholarly journals The role of G-protein-coupled membrane estrogen receptor in mouse Leydig cell function—in vivo and in vitro evaluation

2018 ◽  
Vol 374 (2) ◽  
pp. 389-412 ◽  
Author(s):  
M. Kotula-Balak ◽  
P. Pawlicki ◽  
A. Milon ◽  
W. Tworzydlo ◽  
M. Sekula ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
G Protein ◽  
Leydig Cell ◽  
Cell Function ◽  
G Protein Coupled ◽  
Mouse Leydig Cell ◽  
Leydig Cell Function

The paracrine role of Sertoli cells on Leydig cell function

1992 ◽  
Vol 8 (3) ◽  
pp. 73-83 ◽  
Author(s):  
Herv� Lejeune ◽  
Mourad Skalli ◽  
Pierre G. Chatelain ◽  
Odile Avallet ◽  
Jos� M. Saez
Keyword(s):  
Leydig Cell ◽  
Sertoli Cells ◽  
Cell Function ◽  
Leydig Cell Function

scholarly journals Effect of Short—Term Starvation on Leydig Cell Function in Adult Rats

1997 ◽  
Vol 38 (3) ◽  
pp. 207-214 ◽  
Author(s):  
G. Grizard ◽  
C. Artonne ◽  
J. Grizard ◽  
D. Boucher
Keyword(s):  
Leydig Cell ◽  
Cell Function ◽  
Short Term ◽  
Adult Rats ◽  
Leydig Cell Function

IN VITRO AND IN VIVO STUDIES ON LEYDIG CELL FUNCTION IN OLD RATS

1978 ◽  
Vol 89 (2) ◽  
pp. 393-403 ◽  
Author(s):  
Karl M. Pirke ◽  
Hermann-J. Vogt ◽  
Michael Geiss
Keyword(s):  
Young Adult ◽  
Leydig Cell ◽  
Cell Function ◽  
Binding Capacity ◽  
In Vivo Studies ◽  
Plasma Testosterone ◽  
Dibutyryl Camp ◽  
Adult Rats ◽  
Testicular Weight

ABSTRACT Young adult (3 months old) and old (26–28 months old) male Wistar rats were studied. Testicular weight was 1.66 g (range: 1.33 – 2.86) in the younger group and 1.62 g (range: 1.04 – 1.90) in the older group. The total Leydig cell volume as measured by a quantitative histometric method was significantly larger in the old animals (x̄ = 0.153 ml vs. x̄ = 0.089 ml). The testicular HCG binding capacity was 2.54 ng HCG per 100 mg tissue (range: 1.88 – 3.77) in the younger animals and 1.83 ng HCG per 100 mg tissue (range: 0.80 – 3.02) in the older ones (P < 0.01). Plasma testosterone was on an average 242 ng/100 ml (range: 72 – 1162) in the young adult rats and 91 ng/100 ml (range: 23 – 277) in the older rats. Plasma LH was only slightly (P < 0.05) decreased and was 49 ng LH–RP–1/ml (range: 14 – 120) in the younger group and 40 ng LH–RP–1 ng/ml (range: 5 – 98) in the older. When the testicular tissue pieces were incubated with different doses of HCG or dibutyryl cAMP and testosterone production was measured, identical dose-response curves for old and young tissue were obtained. After in vitro incubation with a NADPH generating system, which under the conditions used was a much stronger stimulus for steroid biosynthesis than HCG or dibutyryl cAMP, the tissue of young rats produced about twice the amount of testosterone and testosterone precursors (pregnenolone, progesterone, 17α-hydroxyprogesterone and androstenedione) than old tissue.

Immune-endocrine interactions and Leydig cell function: the role of cytokines

Andrologia ◽  
2003 ◽  
Vol 35 (1) ◽  
pp. 55-63 ◽  
Author(s):  
T. Diemer ◽  
D. B. Hales ◽  
W. Weidner
Keyword(s):  
Leydig Cell ◽  
Cell Function ◽  
Leydig Cell Function
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