Purified Recombinant VP2 Protein Can Provide Complete Protection to very Virulent Infectious Bursal Disease Virus Challenge as a Subunit Vaccine

AbstractIn order to develop a desirable inexpensive, effective and safe vaccine against the very virulent infectious bursal disease virus (vvIBDV), we tried to take advantage of the emerging T4 bacteriophage surface protein display system. The major immunogen protein VP2 from the vvIBDV strain HK46 was fused to the nonessential T4 phage surface capsid protein, a small outer capsid (SOC) protein, resulting in the 49 kDa SOC-VP2 fusion protein, which was verified by sodium dodecylsulfate polyacrylamide gel electrophoresis and Western blot. Immunoelectromicroscopy showed that the recombinant VP2 protein was successfully displayed on the surface of the T4 phage. The recombinant VP2 protein is antigenic and showed reactivities to various monoclonal antibodies (mAbs) against IBDV, whereas the wild-type phage T4 could not react to any mAb. In addition, the recombinant VP2 protein is immunogenic and elicited specific antibodies in immunized specific pathogen free (SPF) chickens. More significantly, immunization of SPF chickens with the recombinant T4-VP2 phage protected them from infection by the vvIBDV strain HK46. When challenged with the vvIBDV strain HK46 at a dose of 100 of 50% lethal dose (LD50) per chicken 4 weeks after the booster was given, the group vaccinated with the T4-VP2 recombinant phage showed no clinical signs of disease or death, whereas the unvaccinated group and the group vaccinated with the wild-type T4 phage exhibited 100% clinical signs of disease and bursal damages, and 30%-40% mortality. Collectively, the data herein showed that the T4-displayed VP2 protein might be an inexpensive, effective and safe vaccine candidate against vvIBDV.

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A Recombinant Newcastle Disease Virus (NDV) Expressing VP2 Protein of Infectious Bursal Disease Virus (IBDV) Protects against NDV and IBDV

Journal of Virology ◽

10.1128/jvi.78.18.10054-10063.2004 ◽

2004 ◽

Vol 78 (18) ◽

pp. 10054-10063 ◽

Cited By ~ 102

Author(s):

Zhuhui Huang ◽

Subbiah Elankumaran ◽

Abdul S. Yunus ◽

Siba K. Samal

Keyword(s):

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

Recombinant Virus ◽

Infectious Bursal Disease Virus ◽

Antibody Responses ◽

Infectious Bursal Disease ◽

Vp2 Protein ◽

Bursal Disease ◽

Recombinant Newcastle Disease Virus

ABSTRACT Infectious bursal disease virus (IBDV) causes a highly immunosuppressive disease in chickens. Currently available, live IBDV vaccines can lead to generation of variant viruses. We have developed an alternative vaccine that will not create variant IBDV. By using the reverse genetics approach, we devised a recombinant Newcastle disease virus (NDV) vector from a commonly used vaccine strain LaSota to express the host-protective immunogen VP2 of a variant IBDV strain GLS-5. The gene encoding the VP2 protein of the IBDV was inserted into the most 3′-proximal locus of a full-length NDV cDNA for high-level expression. We successfully recovered the recombinant virus, rLaSota/VP2. The rLaSota/VP2 was genetically stable, at least up to 12 serial passages in chicken embryos, and was shown to express the VP2 protein. The VP2 protein was not incorporated into the virions of recombinant virus. Recombinant rLaSota/VP2 replicated to a titer similar to that of parental NDV strain LaSota in chicken embryos and cell cultures. To assess protective efficacy of the rLaSota/VP2, 2-day-old specific-pathogen-free chickens were vaccinated with the recombinant virus and challenged with a highly virulent NDV strain Texas GB or IBDV variant strain GLS-5 at 3 weeks postvaccination. Vaccination with rLaSota/VP2 generated antibody responses against both NDV and IBDV and provided 90% protection against NDV and IBDV. Booster immunization induced higher levels of antibody responses against both NDV and IBDV and conferred complete protection against both viruses. These results indicate that the recombinant NDV can be used as a vaccine vector for other avian pathogens.

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Identification of Neutralizing Epitopes on the VP2 Protein of Infectious Bursal Disease Virus by Phage-Displayed Heptapeptide Library Screening and Synthetic Peptide Mapping

Viral Immunology ◽

10.1089/vim.2005.18.549 ◽

2005 ◽

Vol 18 (3) ◽

pp. 549-557 ◽

Cited By ~ 14

Author(s):

Xuan-Nian Wang ◽

Gai-Ping Zhang ◽

Ji-Yong Zhou ◽

Chun-Hua Feng ◽

Yan-Yan Yang ◽

...

Keyword(s):

Disease Virus ◽

Synthetic Peptide ◽

Infectious Bursal Disease Virus ◽

Peptide Mapping ◽

Infectious Bursal Disease ◽

Library Screening ◽

Vp2 Protein ◽

Bursal Disease ◽

Neutralizing Epitopes

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Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens

Virologica Sinica ◽

10.1007/s12250-015-3680-6 ◽

2016 ◽

Vol 31 (3) ◽

pp. 266-269 ◽

Cited By ~ 1

Author(s):

Flavia Adriana Zanetti ◽

Romina Cardona ◽

Carlos Rodolfo Federico ◽

Silvina Chimeno-Zoth ◽

Gabriela Calamante

Keyword(s):

Disease Virus ◽

Infectious Bursal Disease Virus ◽

Infectious Bursal Disease ◽

Vp2 Protein ◽

Bursal Disease ◽

Canarypox Virus

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Baculovirus Virions Displaying Infectious Bursal Disease Virus VP2 Protein Protect Chickens Against Infectious Bursal Disease Virus Infection

Avian Diseases Digest ◽

10.1637/9733-959711-digest.1 ◽

2011 ◽

Vol 6 (2) ◽

pp. e7-e8

Author(s):

Xin-Gang Xu ◽

De-Wen Tong ◽

Zhi-Sheng Wang ◽

Qi Zhang ◽

Zhao-Cai Li ◽

...

Keyword(s):

Virus Infection ◽

Disease Virus ◽

Infectious Bursal Disease Virus ◽

Infectious Bursal Disease ◽

Vp2 Protein ◽

Bursal Disease

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The expression in plants of an engineered VP2 protein of Infectious Bursal Disease Virus induces formation of structurally heterogeneous particles that protect from a very virulent viral strain

PLoS ONE ◽

10.1371/journal.pone.0247134 ◽

2021 ◽

Vol 16 (2) ◽

pp. e0247134

Author(s):

Carla Marusic ◽

Charifa Drissi Touzani ◽

Alessio Bortolami ◽

Marcello Donini ◽

Claudia Zanardello ◽

...

Keyword(s):

Disease Virus ◽

Infectious Bursal Disease Virus ◽

Neutralizing Antibodies ◽

Sucrose Density Gradient ◽

Bursa Of Fabricius ◽

Infectious Bursal Disease ◽

Structural Protein ◽

Vp2 Protein ◽

Bursal Disease ◽

Specific Pathogen

Infectious Bursal Disease Virus (IBDV), the etiological agent of Gumboro disease, causes mortality and immunosuppression in chickens and major losses to poultry industry worldwide. The IBDV major capsid protein VP2 is considered the best candidate for the production of novel subunit vaccines. This structural protein contains the major conformational epitopes responsible for the induction of IBDV neutralizing antibodies in chickens and has been demonstrated able to form supramolecular structures in yeast and insect cells. The aim of this study was to express an engineered version of the VP2 protein (His-pVP2) to verify its ability to self-assemble into virus-like particles in plants. The recombinant VP2 was transiently expressed by agroinfiltration in Nicotiana benthamiana and transmission electron microscopy of sucrose density gradient fractions revealed the presence of a mixed population of differently shaped particles ranging from spherical capsids, with a diameter between ~25 and ~70 nm, to tubular structures, with variable length (from 100 to 400 nm). The recombinant VP2-based particles when used for the intramuscular immunization of specific-pathogen-free chicks resulted able to induce the production of anti-IBDV specific antibodies at titers comparable to those induced by a commercial vaccine. Moreover, all the immunized birds survived to the challenge with a Moroccan very virulent IBDV strain with no major histomorphological alterations of the Bursa of Fabricius, similarly to what obtained with the commercial inactivated vaccine.

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