Simultanuous DNA and RNA extraction from various tissues of the two oyster species, Crassostrea gigas and Ostrea edulis, for metabarcoding v1 (protocols.io.22aggae)

Molecular testing is becoming an important part of the diagnosis of any patient with cancer. The challenge to laboratories is to meet this need, using reliable methods and processes to ensure that patients receive a timely and accurate report on which their treatment will be based. The aim of this paper is to provide minimum requirements for the management of molecular pathology laboratories. This general guidance should be augmented by the specific guidance available for different tumour types and tests. Preanalytical considerations are important, and careful consideration of the way in which specimens are obtained and reach the laboratory is necessary. Sample receipt and handling follow standard operating procedures, but some alterations may be necessary if molecular testing is to be performed, for instance to control tissue fixation. DNA and RNA extraction can be standardised and should be checked for quality and quantity of output on a regular basis. The choice of analytical method(s) depends on clinical requirements, desired turnaround time, and expertise available. Internal quality control, regular internal audit of the whole testing process, laboratory accreditation, and continual participation in external quality assessment schemes are prerequisites for delivery of a reliable service. A molecular pathology report should accurately convey the information the clinician needs to treat the patient with sufficient information to allow for correct interpretation of the result. Molecular pathology is developing rapidly, and further detailed evidence-based recommendations are required for many of the topics covered here.

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Some biochemical and physiological aspects of growth and gametogenesis inCrassostrea gigasandOstrea edulisgrown at sustained elevated temperatures

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315400046208 ◽

1979 ◽

Vol 59 (1) ◽

pp. 95-110 ◽

Cited By ~ 190

Author(s):

Roger Mann

Keyword(s):

Crassostrea Gigas ◽

Elevated Temperatures ◽

Excretion Rate ◽

Ammonia Excretion ◽

Live Weight ◽

Gonadal Development ◽

Ostrea Edulis ◽

Shell Weight ◽

Histological Assessment ◽

Ammonia Excretion Rate

Crassostrea gigas (Thunberg) andOstrea edulis L.were grown at sustained temperatures of 12°, 15°, 18° and 21°C for a period of 19 weeks. Regular assays of weight specific ammonia excretion rate were made, following which animals were sacrificed for estimation of dry meat weight, dry shell weight, biochemical composition (percentage carbon, nitrogen, carbohydrate, ash) and gonadal development (histological assessment). Crassostrea gigas grew from an intial live weight of 5·2 g to values of 23·5, 28·2, 34·6 and 38·7 g at 120, 150, 180 and 21 °C respectively.

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