Assessment of Human Islet Composition and Acinar Cell Component by Immunofluorescence Staining v1 (protocols.io.brdfm23n)

Assessment of Human Islet Composition and Acinar Cell Component by Immunofluorescence Staining v2

10.17504/protocols.io.b2nfqdbn ◽

2021 ◽

Author(s):

IIDP-HIPP not provided

Keyword(s):

Kidney Diseases ◽

Standard Operating Procedure ◽

Human Islet ◽

Immunofluorescence Staining ◽

Immunofluorescent Staining ◽

Assay Method ◽

Staining Procedure ◽

Cell Component ◽

Qualitative Determination

This Standard Operating Procedure (SOP) is based on the Human Islet Phenotyping Program of the IIDP Immunofluorescence Staining Procedure. This SOP provides the HIPP procedure for immunofluorescent staining, imaging, and analysis of islet preparations. This SOP defines the assay method used by the Human Islet Phenotyping Program (HIPP) for quantitative and qualitative determination of the Purified Human Pancreatic Islet product, post-shipment, manufactured for use in the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)-sponsored research in the Integrated Islet Distribution Program (IIDP).

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Fetal Rat Pancreas: Differentiation of the Acinar Cell Component in Vivo and in Vitro

Diabetes ◽

10.2337/diab.22.8.584 ◽

1973 ◽

Vol 22 (8) ◽

pp. 584-589 ◽

Cited By ~ 15

Author(s):

R. C. McEvoy ◽

O. D. Hegre ◽

R. J. Leonard ◽

A. Lazarow

Keyword(s):

Acinar Cell ◽

Cell Component ◽

Rat Pancreas ◽

Fetal Rat

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Fetal and neonatal rat pancreas in organ culture age-related effects of corticosterone on the acinar cell component

American Journal of Anatomy ◽

10.1002/aja.1001460203 ◽

1976 ◽

Vol 146 (2) ◽

pp. 133-149 ◽

Cited By ~ 5

Author(s):

Robert C. McEvoy ◽

Orion D. Hegre ◽

Arnold Lazarow

Keyword(s):

Organ Culture ◽

Acinar Cell ◽

Neonatal Rat ◽

Cell Component ◽

Rat Pancreas ◽

Culture Age ◽

Age Related

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A complex network of “snake-like tubules” revealed by the NADPase cytochemical reaction in the acinar cell of pancreas

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100113214 ◽

1984 ◽

Vol 42 ◽

pp. 666-667

Author(s):

A.R. Beaudoin ◽

G. Grondin ◽

A. Lord ◽

M. Pelletier

Keyword(s):

Acinar Cell ◽

Lead Acetate ◽

Ultrastructural Localization ◽

Sodium Acetate Buffer ◽

Zymogen Granules ◽

Sprague Dawley ◽

Dense Bodies ◽

Sprague Dawley Rats ◽

Cytochemical Reaction ◽

Cellular Organelles

We have recently described the ultrastructural localization of NADPase activity in the exocrine pancreas of rat. The enzyme was found in the intermediate saccules of the Golgi apparatus, in dense bodies and lysosomes but was absent from zymogen granules. A very intense reaction was noticed in a peculiar structure which was termed “Snake-Like Tubule” (SLT). The purposes of the present study were firstly to delineate SLT distribution in the acinar cell and secondly to define any possible relationship or association with other cellular organelles.NADPase cytochemical reaction was performed on the pancreas of adult Sprague Dawley rats. Small lobules were excised and fixed for 50 min, at 4°C, in 2% glutaraldehyde buffered with 0.1M cacodylate at pH 7.2. Lobules were rinsed several times with the same buffer containing 570 sucrose and cut with a Mcllwayn tissue chopper. Sections were washed several times with buffer and incubated for 2 hr at 37°C in the following medium: 4mM NADPH; 40mM sodium acetate buffer, pH 5.0; 4mM lead acetate and 5% sucrose.

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