scholarly journals Immunofluorescence staining protocol for co-staining of fetuin-A and GFAP in older human autopsy tissue via Tyramide Signal Amplification v1 (protocols.io.syeefte)

protocols.io ◽  
2018 ◽  
Author(s):  
Miriam Heinen

1928 ◽  
Vol 78 (3) ◽  
pp. 625-626
Author(s):  
Victor C. Myers ◽  
James W. Mull


1997 ◽  
Vol 67 (1) ◽  
pp. 103-112 ◽  
Author(s):  
Pádraig M Strappe ◽  
Ting Huei Wang ◽  
Chris Anne McKenzie ◽  
Suzanne Lowrie ◽  
Peter Simmonds ◽  
...  




2005 ◽  
Vol 53 (2) ◽  
pp. 249-252 ◽  
Author(s):  
Seung-won Lee ◽  
Song Eun Lee ◽  
Seong Hyuk Ko ◽  
Eun Kyoung Hong ◽  
Kwang Il Nam ◽  
...  

The tyramide signal amplification (TSA) technique has been shown to detect scarce tissue antigens in light and electron microscopy. In this study we applied the TSA technique at the electron microscopic level to pre-embedding immunocytochemistry. This protocol was compared to the non-amplified protocol. With the TSA protocol, the labeling of GM130, a cis-Golgi matrix protein, was tested in a cell line and found to be highly sensitive and more enhanced than that with the simple protocol. Moreover, the gold particles were well localized to the cis-side of the Golgi apparatus in both the TSA and the simple protocol.





Sign in / Sign up

Export Citation Format

Share Document