MICROPROPAGATION OF REHMANNIA GLUTINOSA AS MEDICINAL PLANT BY SHOOT TIP AND ROOT SEGMENT CULTURE

Althaea rosea, commonly known as Hollyhock is an ornamental and medicinal plant. A. rosea has been reported to contain highest amount of tannins, carbohydrates, cyanide and mucilage. The purpose of this study was to optimize conditions for multiplication of Althaea rosea using shoottip explants. Varying concentrations solo and in combinations of auxins and cytokinins were experimented. The development of micro-propagation protocol for this valuable medicinal plant can ensure continuous supply of the plant product for pharmaceutical and food industry. In this regard an efficient and reproducible micro-propagation protocol has been developed using Murashige and Skoog’s (MS) (1962) medium augmented with different phytohormonal combinations like BAP, NAA, IBA and IAA. Both direct and indirect multiplication was obtained using in vitro raised shoot tip explants. Maximum shoot number was obtained using combination of BAP and NAA and rooting of isolated shoots was obtained using IBA. 60% of the transplanted plantlets survived under laboratory conditions.

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In vitro Mass Propagation from Shoot Tip Explants of Vernonia cinerea (L.) Less. An Antioxidant, Anti-inflammatory Medicinal Plant

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v20i2.6892 ◽

1970 ◽

Vol 20 (2) ◽

pp. 127-131 ◽

Cited By ~ 1

Author(s):

M. Maharajan ◽

Abdul Bakrudeen Ali Ahmed ◽

Rosna Mat Taha ◽

S. Jawahar ◽

R. Ravi Paul ◽

...

Keyword(s):

Medicinal Plant ◽

Key Words ◽

Plant Tissue ◽

Shoot Tip ◽

Mass Propagation ◽

Anti Inflammatory ◽

Shoot Tip Explants ◽

Vernonia Cinerea ◽

Number Of Shoots

Plantlets were regenerated from the shoot tip explants of Vernonia cinerea (L.) Less. in MS supplimented with BA and Kn. Maximum number of shoots in BA (13.32 mg/l) and roots in IBA (7.38 mg/l) developed. The rooted plantlets were successfully established in the field. Key words: Vernonia cinerea, Mass propagation, Shoot tip explants, Antioxidant, Medicinal plant D.O.I. 10.3329/ptcb.v20i2.6892 Plant Tissue Cult. & Biotech. 20(2): 127-131, 2010 (December)

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An EfficientIn VitroPlantlet Regeneration from Shoot Tip Cultures ofCurculigo latifolia, a Medicinal Plant

The Scientific World JOURNAL ◽

10.1155/2014/275028 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Nahid Babaei ◽

Nur Ashikin Psyquay Abdullah ◽

Ghizan Saleh ◽

Thohirah Lee Abdullah

Keyword(s):

Medicinal Plant ◽

Butyric Acid ◽

Shoot Length ◽

Shoot Tip ◽

Direct Regeneration ◽

Root Induction ◽

Ms Medium ◽

Shoot Tip Culture ◽

Shoot Number

A procedure was developed forin vitropropagation ofCurculigo latifoliathrough shoot tip culture. Direct regeneration and indirect scalp induction ofCurculigo latifoliawere obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explants producing shoot, shoot number, and shoot length was obtained on MS medium supplemented with combination of thidiazuron (0.5 mg L−1) and indole-3-butyric acid (0.25 mg L−1) after both 10 and 14 weeks of cultures. Indole-3-butyric acid in combination with thidiazuron exhibited a synergistic effect on shoot regeneration. The shoot tips were able to induce maximum scalp from basal end of explants on the medium with 2 mg L−1thidiazuron. Cultures showed that shoot number, shoot length, and scalp size increased significantly after 14 weeks of culture. Transferring of the shoots onto the MS medium devoid of growth regulators resulted in the highest percentage of root induction and longer roots, while medium supplemented with 0.25 mg L−1IBA produced more numbers of roots.

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