In vitro propagation of Althaea rosea - a valuable medicinal plant of Kashmir Himalaya

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Sumira Tyub ◽  
Azra N. Kamili ◽  
Mohammad Mansoor Bhat
Keyword(s):  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Food Industry ◽  
Shoot Tip ◽  
Kashmir Himalaya ◽  
Plant Product ◽  
Shoot Number ◽  
Micro Propagation ◽  
Shoot Tip Explants

Althaea rosea, commonly known as Hollyhock is an ornamental and medicinal plant. A. rosea has been reported to contain highest amount of tannins, carbohydrates, cyanide and mucilage. The purpose of this study was to optimize conditions for multiplication of Althaea rosea using shoottip explants. Varying concentrations solo and in combinations of auxins and cytokinins were experimented. The development of micro-propagation protocol for this valuable medicinal plant can ensure continuous supply of the plant product for pharmaceutical and food industry. In this regard an efficient and reproducible micro-propagation protocol has been developed using Murashige and Skoog’s (MS) (1962) medium augmented with different phytohormonal combinations like BAP, NAA, IBA and IAA. Both direct and indirect multiplication was obtained using in vitro raised shoot tip explants. Maximum shoot number was obtained using combination of BAP and NAA and rooting of isolated shoots was obtained using IBA. 60% of the transplanted plantlets survived under laboratory conditions.

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals IN VITRO PROPAGATION OF ALGERIAN IVY (HEDERA CANARIENSIS L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1123g-1124
Author(s):  
Karim H. Al-Juboory ◽  
David J. Williams
Keyword(s):  
Root Length ◽  
In Vitro Propagation ◽  
Inverse Relationship ◽  
Basal Medium ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Explants ◽  
Strength Medium

Shoot tip explants of Algerian Ivy Heder a canariensis were cultured on MS basal medium supplemented with a combination of salt strength and NAA and IBA. More roots per explant developed on full salt strength medium combined with NAA. The most roots per explant were obtained with a combination of IBA and 1/4 MS salt. There was an inverse relationship between an increase in IBA or NAA concentration and root length and number. Shoots proliferated better on full MS salt combined with NAA and IBA. The highest level of NAA (40 uM) and 0.1 uM TDZ produced the most shoots and roots, the longest roots, the highest rooting percentage, the largest plants with the most leaves and the best callus quality per explant. The leaves from in vitro were cultured on MS medium with varying levels of Thidiazuron (TDZ) and NAA in the presence of light produced the highest number of roots.

scholarly journals In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants

2009 ◽  
Vol 18 (1) ◽  
pp. 1-6 ◽  
Author(s):  
H. David Raja ◽  
D.I. Arockiasamy
Keyword(s):  
High Frequency ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Root Induction ◽  
Root Proliferation ◽  
Key Words Micropropagation ◽  
Shoot Tip Explants ◽  
Important Medicinal Plant

A simple micropropagation method from nodal and shoot tip explants is reported here for Mentha viridis, an economically important medicinal plant. High frequency of microshoots was obtained from these two explants on MS supplemented with various concentrations of BAP (1.0 - 4.0 mg/l) and Kn (1.0 -4.0 mg/l). Maximum number of shoots was obtained from nodal explants in the medium containing 3.0 mg/l BAP. The root induction was carried out by using IBA and IAA (0.5 - 2.0 mg/l). Among these, high frequency of root proliferation was achieved in the medium containing 1.5 mg/l of IBA. The rooted plantlets were hardened and transferred to the field. The survival rate was 90 - 95% after 25 days. Key words: Micropropagation, Mentha viridis, Node, Shoot tip, Multiple shoots D.O.I. 10.3329/ptcb.v18i1.3243 Plant Tissue Cult. & Biotech. 18(1): 1-6, 2008 (June)

scholarly journals In vitro Mass Propagation from Shoot Tip Explants of Vernonia cinerea (L.) Less. An Antioxidant, Anti-inflammatory Medicinal Plant

1970 ◽  
Vol 20 (2) ◽  
pp. 127-131 ◽  
Author(s):  
M. Maharajan ◽  
Abdul Bakrudeen Ali Ahmed ◽  
Rosna Mat Taha ◽  
S. Jawahar ◽  
R. Ravi Paul ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Key Words ◽  
Plant Tissue ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Anti Inflammatory ◽  
Shoot Tip Explants ◽  
Vernonia Cinerea ◽  
Number Of Shoots

Plantlets were regenerated from the shoot tip explants of Vernonia cinerea (L.) Less. in MS supplimented with BA and Kn. Maximum number of shoots in BA (13.32 mg/l) and roots in IBA (7.38 mg/l) developed. The rooted plantlets were successfully established in the field.   Key words: Vernonia cinerea, Mass propagation, Shoot tip explants, Antioxidant, Medicinal plant   D.O.I. 10.3329/ptcb.v20i2.6892   Plant Tissue Cult. & Biotech. 20(2): 127-131, 2010 (December)

scholarly journals An EfficientIn VitroPlantlet Regeneration from Shoot Tip Cultures ofCurculigo latifolia, a Medicinal Plant

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Nahid Babaei ◽  
Nur Ashikin Psyquay Abdullah ◽  
Ghizan Saleh ◽  
Thohirah Lee Abdullah
Keyword(s):  
Medicinal Plant ◽  
Butyric Acid ◽  
Shoot Length ◽  
Shoot Tip ◽  
Direct Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Tip Culture ◽  
Shoot Number

A procedure was developed forin vitropropagation ofCurculigo latifoliathrough shoot tip culture. Direct regeneration and indirect scalp induction ofCurculigo latifoliawere obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explants producing shoot, shoot number, and shoot length was obtained on MS medium supplemented with combination of thidiazuron (0.5 mg L−1) and indole-3-butyric acid (0.25 mg L−1) after both 10 and 14 weeks of cultures. Indole-3-butyric acid in combination with thidiazuron exhibited a synergistic effect on shoot regeneration. The shoot tips were able to induce maximum scalp from basal end of explants on the medium with 2 mg L−1thidiazuron. Cultures showed that shoot number, shoot length, and scalp size increased significantly after 14 weeks of culture. Transferring of the shoots onto the MS medium devoid of growth regulators resulted in the highest percentage of root induction and longer roots, while medium supplemented with 0.25 mg L−1IBA produced more numbers of roots.

scholarly journals In vitro Shoot Multiplication of Bupleurum disticho-phyllum Wight - A Native Medicinal Plant of Southern India

1970 ◽  
Vol 17 (2) ◽  
pp. 115-124 ◽  
Author(s):  
S. Karuppusamy ◽  
T. Pullaiah
Keyword(s):  
Medicinal Plant ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Shoot Tip ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Axillary Shoot Proliferation ◽  
Shoot Tip Explants

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was found to be 74 and 70% for nodal- and shoot tip explants, respectively on the medium containing 1.0 mg/l BAP + 0.1 mg/l NAA. The combination of BAP and GA3 was also found to be effective for both type of explants. The degree of shoot formation was affected by explant types and the exogenous hormonal regime in the medium. The regenerated shoots were successfully rooted on MS supplemented with 2.0 mg/l IBA, after sequential hardening, survival rate was 71%. Key words: Bupleurum distichophyllum, Medicinal plant, Micropropagation, Conservation Plant Tissue Cult. & Biotech. 17(2): 115-124, 2007 (December) DOI: 10.3329/ptcb.v17i2.2574

A rapid in vitro propagation of red sanders (Pterocarpus santalinus L.) using shoot tip explants

2011 ◽  
Vol 33 (6) ◽  
pp. 2501-2510 ◽  
Author(s):  
K. Balaraju ◽  
P. Agastian ◽  
S. Ignacimuthu ◽  
Kyungseok Park
Keyword(s):  
In Vitro Propagation ◽  
Shoot Tip ◽  
Pterocarpus Santalinus ◽  
Red Sanders ◽  
Shoot Tip Explants

scholarly journals IN VITRO PROPAGATION OF ALGERIAN IVY (HEDERA CANARIENSIS L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1123G-1124 ◽  
Author(s):  
Karim H. Al-Juboory ◽  
David J. Williams
Keyword(s):  
Root Length ◽  
In Vitro Propagation ◽  
Inverse Relationship ◽  
Basal Medium ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Explants ◽  
Strength Medium

Shoot tip explants of Algerian Ivy Heder a canariensis were cultured on MS basal medium supplemented with a combination of salt strength and NAA and IBA. More roots per explant developed on full salt strength medium combined with NAA. The most roots per explant were obtained with a combination of IBA and 1/4 MS salt. There was an inverse relationship between an increase in IBA or NAA concentration and root length and number. Shoots proliferated better on full MS salt combined with NAA and IBA. The highest level of NAA (40 uM) and 0.1 uM TDZ produced the most shoots and roots, the longest roots, the highest rooting percentage, the largest plants with the most leaves and the best callus quality per explant. The leaves from in vitro were cultured on MS medium with varying levels of Thidiazuron (TDZ) and NAA in the presence of light produced the highest number of roots.

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