INFLUENCE OF GELLING AGENTS ON ILEX PARAGUARIENSIS TISSUE CULTURE

2001 ◽  
pp. 453-456 ◽  
Author(s):  
P.A. Sansberro ◽  
H.Y. Rey ◽  
C.V. Luna ◽  
L.A. Mroginski
Keyword(s):  
Tissue Culture ◽  
Ilex Paraguariensis ◽  
Gelling Agents

Use of low-cost gelling agents and support matrices for industrial scale plant tissue culture

1994 ◽  
Vol 37 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Parthasarathi Bhattacharya ◽  
Satyahari Dey ◽  
Bimal Chandra Bhattacharyya
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Low Cost ◽  
Industrial Scale ◽  
Gelling Agents

scholarly journals Vessel Volume, Gelling Agent, and Basal Salts Affect pH and Gel Strength of Autoclave Tissue Culture Media

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 683-685 ◽  
Author(s):  
Hazel Y. Wetzstein ◽  
Choongsik Kim ◽  
Harry E. Sommer
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Basal Medium ◽  
Gel Strength ◽  
Gelling Agent ◽  
Mean Values ◽  
Gelling Agents ◽  
Tissue Culture Media ◽  
Vessel Volume ◽  
Basal Salts

Effects of autoclaving volume, gelling agent (Bactoagar, Gel-gro, Phytagar), and basal salts [Murashige and Skoog (MS); Woody Plant Medium (WPM); Gamborg B5 (GB)] on gel strength and pH of tissue culture media were tested. Gel strength was significantly affected by gelling agent and basal medium. MS media were generally softer than comparable WPM or GB media. As the vessel volume during autoclaving decreased, gel strength significantly decreased with Phytagar and Bactoagar gelling agents; Gel-gro had greater gel strength at the intermediate volume of medium autoclave. In all cases, autoclaving resulted in a pH decrease of 0.2 to 0.5 pH units. Lower pH values were associated with softer gels. The type of gelling agent did not greatly affect the postautoclave pH; mean values among gelling agents were within 0.05 pH units. Postautoclave pH of MS medium was lower than that of WPM or GB. This study verifies the need to observe uniform sterilization protocols to maintain consistency in the chemical and physical properties of media.

scholarly journals Admixture of Isubgol husk together with conventionally used agar as gelling agent for Potato and Stevia regeneration

2012 ◽  
Vol 47 (2) ◽  
pp. 161-166
Author(s):  
Salim Khan ◽  
Abdullah Al Maruf ◽  
Shahina Aktera ◽  
Ahashan Habiba ◽  
Tanjina Akhtar Banua ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Culture Media ◽  
Gelling Agent ◽  
Plantago Ovata ◽  
Gelling Agents ◽  
Tissue Culture Media ◽  
Commercially Important ◽  
The Cost

Attempts have been made to find a substitute for agar as gelling agent. The study was conducted on commercially important crop plants like    potato varieties (Diamond and Granola) and Stevia. 'Isubgol', the mucilaginous husk, derived from Plantago ovata was successfully used as a gelling material in tissue culture media. The price of 'Isubgol husk' is cheaper than the conventionally used agar and it had reduced the price of gelling agent approximately by 47.5 % in plant tissue culture media. The use of 'Isubgol husk' along with agar can reduce the cost of gelling agents. The response from media gelled with Isubgol husk in potato and Stevia was similar to that from media solidified with agar. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11447 Bangladesh J. Sci. Ind. Res. 47(2), 161-166, 2012

scholarly journals Polyamines, Gelling Agents in Tissue Culture, Micropropagation of Medicinal Plants and Bioreactors

10.5772/51028 ◽  
2012 ◽  
Author(s):  
Giuseppina Pace Pereira Lima ◽  
Rene Arnoux da Silva Campos ◽  
Lilia Gomes ◽  
Terezinha J.R. ◽  
Fabio Vianello
Keyword(s):  
Tissue Culture ◽  
Medicinal Plants ◽  
Gelling Agents

scholarly journals Influence of Gelling Agents on Growth of Plant Tissue Culture.

1995 ◽  
Vol 12 (2) ◽  
pp. 215-219
Author(s):  
Iwao ASAI ◽  
Toshiro OOMOTO ◽  
Kunitoshi YOSHIHIRA
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Gelling Agents

scholarly journals Roles of Gelling Agents in Plant Tissue Culture

1986 ◽  
Vol 3 (1) ◽  
pp. 38-41 ◽  
Author(s):  
Koichiro SHIMOMURA ◽  
Hiroshi KAMADA
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Gelling Agents

scholarly journals Using Polysaccharides and Galactomannans as Gelling Agents in Capsule Formation of Artificial Seeds

1997 ◽  
Vol 122 (6) ◽  
pp. 878-883 ◽  
Author(s):  
Seppo Sorvari ◽  
Otto Toldi ◽  
Katriina Ahanen ◽  
Tuula Viinamäki ◽  
Tiina Hakonen ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Daucus Carota ◽  
Somatic Embryos ◽  
Calcium Alginate ◽  
Culture Conditions ◽  
Blue Color ◽  
Artificial Seeds ◽  
Capsule Formation ◽  
Gelling Agents ◽  
Cell And Tissue Culture

Somatic embryos that are produced in cell and tissue culture conditions do not form endosperm and testa around the embryo. For many envisioned applications, delicate embryos need to be covered with an artificial endosperm-testa matrix that allows for respiration, contains nutrients and protectants, and is hard enough but allows the vague embryo to germinate and grow into a plant. A number of industrially produced and commonly available compounds were tested to determine their efficacy in supporting the germination of encapsulated somatic embryos of carrot (Daucus carota L.). Most of the combinations of galactomannans and polysaccharides were at least as effective as calcium alginate alone in germinating the embryos. The disappearance of blue color in Lugol staining around embryos indicates that starch might also provide nourishment to embryos, thus serving as an artificial endosperm and a supportive matrix.

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