scholarly journals Admixture of Isubgol husk together with conventionally used agar as gelling agent for Potato and Stevia regeneration

2012 ◽  
Vol 47 (2) ◽  
pp. 161-166
Author(s):  
Salim Khan ◽  
Abdullah Al Maruf ◽  
Shahina Aktera ◽  
Ahashan Habiba ◽  
Tanjina Akhtar Banua ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Culture Media ◽  
Gelling Agent ◽  
Plantago Ovata ◽  
Gelling Agents ◽  
Tissue Culture Media ◽  
Commercially Important ◽  
The Cost

Attempts have been made to find a substitute for agar as gelling agent. The study was conducted on commercially important crop plants like    potato varieties (Diamond and Granola) and Stevia. 'Isubgol', the mucilaginous husk, derived from Plantago ovata was successfully used as a gelling material in tissue culture media. The price of 'Isubgol husk' is cheaper than the conventionally used agar and it had reduced the price of gelling agent approximately by 47.5 % in plant tissue culture media. The use of 'Isubgol husk' along with agar can reduce the cost of gelling agents. The response from media gelled with Isubgol husk in potato and Stevia was similar to that from media solidified with agar. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11447 Bangladesh J. Sci. Ind. Res. 47(2), 161-166, 2012

scholarly journals Vessel Volume, Gelling Agent, and Basal Salts Affect pH and Gel Strength of Autoclave Tissue Culture Media

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 683-685 ◽  
Author(s):  
Hazel Y. Wetzstein ◽  
Choongsik Kim ◽  
Harry E. Sommer
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Basal Medium ◽  
Gel Strength ◽  
Gelling Agent ◽  
Mean Values ◽  
Gelling Agents ◽  
Tissue Culture Media ◽  
Vessel Volume ◽  
Basal Salts

Effects of autoclaving volume, gelling agent (Bactoagar, Gel-gro, Phytagar), and basal salts [Murashige and Skoog (MS); Woody Plant Medium (WPM); Gamborg B5 (GB)] on gel strength and pH of tissue culture media were tested. Gel strength was significantly affected by gelling agent and basal medium. MS media were generally softer than comparable WPM or GB media. As the vessel volume during autoclaving decreased, gel strength significantly decreased with Phytagar and Bactoagar gelling agents; Gel-gro had greater gel strength at the intermediate volume of medium autoclave. In all cases, autoclaving resulted in a pH decrease of 0.2 to 0.5 pH units. Lower pH values were associated with softer gels. The type of gelling agent did not greatly affect the postautoclave pH; mean values among gelling agents were within 0.05 pH units. Postautoclave pH of MS medium was lower than that of WPM or GB. This study verifies the need to observe uniform sterilization protocols to maintain consistency in the chemical and physical properties of media.

Vegetable Waste as an Alternate Plant Tissue Culture Media for Laboratory and Industry

2019 ◽  
Vol 12 (4) ◽  
pp. 1521
Author(s):  
R. Subbaiya ◽  
B. Aakash ◽  
A. Shanmugaraja ◽  
R. Devika ◽  
S. Chozhavendhan ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Culture Media ◽  
Vegetable Waste ◽  
Tissue Culture Media
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