Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

Plant tissue culture plays an important role in plant biotechnology due to its potential for massive production of improved crop varieties and high yield of important secondary metabolites. Several efforts have been made to ameliorate the effectiveness and production of plant tissue culture, using biotic and abiotic factors. Nowadays, the addition of nanoparticles as elicitors has, for instance, gained worldwide interest because of its success in microbial decontamination and enhancement of secondary metabolites. Nanoparticles are entities in the nanometric dimension range: they possess unique physicochemical properties. Among all nanoparticles, silver-nanoparticles (AgNPs) are well-known for their antimicrobial and hormetic effects, which in appropriate doses, led to the improvement of plant biomass as well as secondary metabolite accumulation. This review is focused on the evaluation of the integration of nanotechnology with plant tissue culture. The highlight is especially conveyed on secondary metabolite enhancement, effects on plant growth and biomass accumulation as well as their possible mechanism of action. In addition, some perspectives of the use of nanomaterials as potential therapeutic agents are also discussed. Thus, the information provided will be a good tool for future research in plant improvement and the large-scale production of important secondary metabolites. Elicitation of silver-nanoparticles, as well as nanomaterials, function as therapeutic agents for animal well-being is expected to play a major role in the process. However, nanosized supramolecular aggregates have received an increased resonance also in other fields of application such as animal welfare. Therefore, the concluding section of this contribution is dedicated to the description and possible potential and usage of different nanoparticles that have been the object of work and expertise also in our laboratories.

Aklimatisasi Anggrek Species Hasil Kultur Jaringan Melalui Pemberdayaan Masyarakat Dusun Gempol

Gempol Village is a village on Mount Ungaran that has made efforts to preserve orchid species. Through mentoring and training from the UNNES Research Team which began in 2011, as well as greenhouse facilitation from PT Indonesia Power, the Gempol village community who are members of the Omah Sawah Community began to make efforts to conserve orchid species. The results of the identification of the problems experienced by community groups as foster partners can be grouped into three aspects, namely knowledge and skills of acclimatization of orchids from plant tissue culture, post-acclimatization management/care, and supporting infrastructure for acclimatization of tissue cultured orchids, where these three aspects are interrelated. The methods used include lecture and question and answer activities, practice, and mentoring. The result achieved is that the orchid species acclimatization activity in Gempol Hamlet, Ngesrepbalong Village has been carried out with satisfactory results. Activities are carried out through training and assistance to community groups who are members of Omah Sawah. The results of the evaluation of the participants showed that the participants' understanding and skills improved after this activity was carried out, even providing ideas for participants to apply to cultivated orchids. The result is enough to generate economic income for the participants, because some of their cultivated orchids are sold. Abstrak Dusun Gempol adalah satu dusun di Gunung Ungaran yang telah melakukan upaya pelestarian anggrek species hutan . Melalui pendampingan dan pelatihan dari Tim Peneliti UNNES yang dimulai pada tahun 201, serta fasilitasi greenhouse dari PT Indonesia Power, masyarakat Dusun Gempol yang tergabung dalam Komunitas Omah Sawah mulai melakukan upaya pelestarian anggrek species. Hasil identifikasi terhadap permasalahan yang dialami kelompok masyarakat sebagai mitra binaan dapat dikelompokkan menjadi tiga aspek, yaitu pengetahuan dan keterampilan aklimatisasi anggrek hasil kultur jaringan tanaman, pengelolaan/ perawatan pasca aklimatisasi, dan sarana prasarana penunjang aklimatisasi anggrek hasil kutur jaringan, dimana ke tiga aspek ini salingterkait. Metode yang dilakukan, meliputi kegiatan ceramah dan tanya jawab, praktik, dan pendampingan. Hasil yang dicapai adalah kegiatan aklimatisasi anggrek species di Dusun Gempol Desa Ngesrepbalong telah dilaksanakan dengan hasil yang memuaskan. Kegiatan dilaksanakan melalui pelatihan dan pendampingan terhadap kelompok masyarakat yang tergabung di Omah Sawah. Hasil evaluasi terhadap peserta menunjukkan bahwa pemahaman dan ketrampilan peserta meningkat setelah dilakukan kegiatan ini, bahkan memberikan ide bagi peserta untuk menerapkan pada anggrek budidaya. Hasilnya cukup membuahkan pemasukan ekonomi bagi peserta, karena beberapa anggrek budidaya mereka laku dijual.

Effect of LED Lighting on Physical Environment and Microenvironment on In Vitro Plant Growth and Morphogenesis: The Need to Standardize Lighting Conditions and Their Description

In the last decades, lighting installations in plant tissue culture have generally been renewed or designed based on LED technology. Thanks to this, many different light quality advances are available but, with their massive implementation, the same issue is occurring as in the 1960s with the appearance of the Grolux (Sylvania) fluorescent tubes: there is a lack of a methodological standardization of lighting. This review analyzes the main parameters and variables that must be taken into account in the design of LED-based systems, and how these need to be described and quantified in order to homogenize and standardize the experimental conditions to obtain reproducible and comparable results and conclusions. We have designed an experimental system in which the values of the physical environment and microenvironment conditions and the behavior of plant tissue cultures maintained in cabins illuminated with two lighting designs can be compared. Grolux tubes are compared with a combination of monochromatic LED lamps calibrated to provide a spectral emission, and light irradiance values similar to those generated by the previous discharge lamps, achieving in both cases wide uniformity of radiation conditions on the shelves of the culture cabins. This study can help to understand whether it is possible to use LEDs as one standard lighting source in plant tissue culture without affecting the development of the cultures maintained with the previously regulated protocols in the different laboratories. Finally, the results presented from this caparison indicate how temperature is one of the main factors that is affected by the chosen light source.

Integration of Nanotechnology in Plant Tissue Culture

: In the field of plant biology, tissue culture is having colossal applications, for example, the production of disease-free plants and their mass multiplication, germplasm preservation, genetic manipulation to get improved variety as well as the production of biologically active compounds. The integration of nanotechnology and application of nanoparticles (NPs) has shown a positive response in the elimination of microbial contaminants and induction of callus, somatic embryogenesis, organogenesis, production of secondary metabolites, and genetic transformation. This paper aims to highlight some of the recent advancements that came possible through the implementation of nanotechnology in the field of plant tissue culture and also discusses both positives and negatives aspects associated with NPs in plant tissue culture. The prospects through the involvement of recent innovations of nanotechnology such as dendrimers, quantum dots, and carbon nanotubes are also proposed.

Plant Tissue culture and Ultra High Diluted studies: suggesting a novel model using in vitro techniques

Plant tissue culture techniques have been used to evaluate the effects of many different substances and/ or conditions in plant growth and development. It provides information of great value about problems related to basic and applied aspects of plant as well as contributed to understanding of factors responsible for growth, metabolism, synthesis of secondary compounds, stress response. Considering all this wide range of applications and as all plant tissue culture techniques are undergone under axenic and controlled conditions (culture medium composition, light and temperature, for instance), it seems to be a value model for Ultra High Diluted (UHD) studies. Lippia alba is a Brazilian plant that tissue cultures protocols and in vitro essential oil production have already been described in scientific literature. None of all scientific papers evaluated the effects of UHD substances on in vitro development or secondary metabolic production. The main goal was to evaluate the use of plant tissue culture to investigate the effects of UHD benzilaminopurine (BA) on Lippia alba shoot culture. Nodal segments obtained from plants growth in vitro was subcultured to Murashigue & Skoog semi-solid medium added with 2ml of these different solutions: BA 3µmol, BA 12CH (10-24), water 12CH and water (no dilution and succussion). Weekly 1 ml of solutions were added to cultures. The experiment was repeated twice and each one consisted in 3 culture vessel with 5 nodal segments per treatment (n=30). All plants were maintained in growth room under controlled temperature (25°C), light and photoperiod (16L/8D). The tested substances were prepared according to the method of stepwise dilution and succussion as describe in Brazilian Homeopathic Pharmacopoeia. The experiment was blinded all the time. After 60d, plantlets were evaluated for number of shoots, shoot length, rooted plants (%), callus development (%) and fresh biomass. Data were submitted to ANOVA following by Duncan’s and t-test. Plants from water 12CH and BA 12CH increased the number of new shoots and promoted the highest shoot length. By adding BA 3µmol the organogenetic response was inhibited since neither shoot nor root were developed. However, it was observed a significant basal callus development. Plant tissue culture could be adapted for UHD studies. More studies are being conducted in way to analyze other experimental conditions and biochemical/phytochemical parameters.

An Overview of Oil Palm Cultivation via Tissue Culture Technique

During the last three decades, plant cell, tissue, and organ culture have developed rapidly and become a major biotechnology tool in agriculture, horticulture, forestry, and industry. Many problems in conventional breeding techniques were solved via tissue culture techniques. Plant tissue culture technique permits the growing plants in test tube or closed container in vitro under controlled environment. This technique is devoted to solve two problems: 1) To keep the plant cells free from microbes. 2) To grow the desired plants by providing suitable nutrient medium and other environmental conditions. In this chapter, a review around plant tissue culture techniques that have been reported on oil palm breeding programme will be discussed. It is including the laboratory techniques, advantages and disadvantages of the technique, the problems to produce good and prolific oil palm tissue culture clones and mitigation measures that have been reported to overcome the problems. As a conclusion, this chapter reviews tissue culture techniques that could be used to propagate oil palm clones.