SEX DETERMINATION OF KIWIFRUIT SEEDLINGS WITH MOLECULAR MARKERS

2014 ◽  
pp. 197-203
Author(s):  
A. Atak ◽  
B. Aydin ◽  
K. Abdurrahim Kahraman
2020 ◽  
Vol 42 ◽  
pp. e45
Author(s):  
Marília Pereira Machado ◽  
Andreza Cerioni Belniaki ◽  
André Felipe Bernert ◽  
Erik Nunes Gomes ◽  
João Carlos Bespalhok Filho ◽  
...  

Brazil is the world's third largest beer consumer and currently imports all of its hops for the brewing industry. Such a fact justifies the selection of hop genotypes adapted for cultivation locally, which requires high quality seeds and efficient sex determination of the seedlings. The objectives of this study were to develop a methodology to assess hop seed quality and to efficiently determine hop seedling sex through the use of male-specific molecular markers. Freshly harvested hop seeds were germinated with and without pre-chilling (3-5 ° C) for 3, 6 and 12 weeks and then germinated at 20 or 25 ° C in the presence or absence of light, evaluating germination percentage and germination speed index. F1 progenies were obtained from after seed germination in a greenhouse and seedlings sex was determined using male-specific molecular markers. The best conditions for physiological quality assessment of hop seeds used in the present study were pre-chilling for 12 weeks, followed by germination at 25 ° C, and normal seedling counts at 7 and 15 days. The progeny submitted to molecular marker sexing was composed of 61.3% female plants. The established methodologies presented here can be considered efficient and may contribute to expedite hops breeding programs.


2020 ◽  
Vol 21 (1) ◽  
pp. 55
Author(s):  
Indriawati Indriawati ◽  
Slamet Diah Volkandari ◽  
Endang Tri Margawati

An investigation involving large number of animals is often resulting incomplete or in accurate information such as animal parentage, or misidentify on sex due to unlabeled sex samples. A PCR method by applying Y chromosome markers (UTY and SRY) facilitates in determination of unknown sex problem. This study was intended to determine sex from unlabelled sex of blood samples by applying PCR method using a pooled-DNA template. Twenty five of unknown sex blood samples from Nusa Penida, Bali were used in this study. The samples were plotted into 5 pooled-DNA whith each pool DNA consisted of 5 individuals DNA. Two pairs of sex primers, UTY (58oC) and SRY (60oC) with 35 cycles were applied to amplify the samples. The result showed there was only one pooled-DNA (P4) amplified by UTY (484bp). Whereas re-PCR of the positive pooled-DNA (P4) using SRY primer, only one out of 25 samples determined as male Bali cattle (325bp). This finding suggests that UTY and SRY primers are suitable for sex determination and the pooled-DNA could be used as an efficient PCR method both in consumables and PCR process for sex determination. Keywords: Determination, sex, unknown sample, pooled DNA, Bali cattle.


2014 ◽  
Vol 13 (1) ◽  
pp. 50-59
Author(s):  
A NisreenYasirJasim ◽  
Tahir A. Fahid ◽  
Talib Ahmed Jaayid

2012 ◽  
Vol 3 (1) ◽  
pp. 384-386
Author(s):  
Dr. Sudarshan Gupta ◽  
◽  
Dr. Bhadresh Vaghela ◽  
Dr. Deepak Howale ◽  
Dr. Mehul Tandel

2010 ◽  
Vol 5 (2) ◽  
pp. 59-66
Author(s):  
Kelven Lopes ◽  
Helder Lima de Queiroz

This study examined the traditional knowledge of fishermen at Mamirauá Reserve about sex determination of pirarucus, Arapaima gigas Schinz 1822. We evaluated the criteria used for by fishermen in the sector Jarauá at Mamirauá Reserve for this determination, during the fisheries season, from October to November 2004. We analyzed responses of a group of about 15 fishermen collected in structured interviews regarding 109 individual pirarucus, 65 males and 44 females. From this sample, only 64 fish have their sex correctly predicted by the fishermen. Although the traditional knowledge of local fishermen is a key component of the sustainable fisheries of this species, this knowledge is not functional in all ranges and aspects, as in sex distinction. We found that the local fishermen evaluated are not able to recognize the sex of pirarucus, although some criteria applied for this purpose are consistent with their biology. The rates of correct forecasts for recognition of males and females were similar to those obtained by chance, even when the criteria applied were consistent, as the criterion “coloration”, which was significantly consistent for identification of males. Yet the group of fishermen interviewed in this study apparently did not apply this criterion correctly.


1989 ◽  
Vol 30 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Birthe Avery ◽  
Mette Schmidt

1989 ◽  
Vol 30 (2) ◽  
pp. 147-153
Author(s):  
Birthe Avery ◽  
Mette Schmidt ◽  
Torben Greve

2011 ◽  
Vol 73 (12) ◽  
pp. 1611-1616 ◽  
Author(s):  
Shoki YAMAZAKI ◽  
Yuta MOTOI ◽  
Kazuya NAGAI ◽  
Tsuyoshi ISHINAZAKA ◽  
Makoto ASANO ◽  
...  

Food Control ◽  
2010 ◽  
Vol 21 (5) ◽  
pp. 726-731 ◽  
Author(s):  
W.L. Bai ◽  
R.H. Yin ◽  
S.J. Zhao ◽  
C. Li ◽  
Z.J. Ma ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document