Targeting of RET oncogene by naphthalene diimide-mediated gene promoter G-quadruplex stabilization exerts anti-tumor activity in oncogene-addicted human medullary thyroid cancer

An activated ras oncogene induces a program of differentiation in the human medullary thyroid cancer cell line TT. This differentiation process is accompanied by a marked increase in the transcription of the human calcitonin (CT) gene. We have localized a unique Ras-responsive transcriptional element (RRE) in the CT gene promoter. DNase I protection indicates two domains of protein-DNA interaction, and each domain separately can confer Ras-mediated transcriptional inducibility. This bipartite RRE was also found to be Raf responsive. By affinity screening, we have cloned a cDNA coding for a zinc finger transcription factor (RREB-1) that binds to the distal RRE. The consensus binding site for this factor is CCCCAAACCACCCC. RREB-1 is expressed ubiquitously in human tissues outside the adult brain. Overexpression of RREB-1 protein in TT cells confers the ability to mediate increased transactivation of the CT gene promoter-reporter construct during Ras- or Raf-induced differentiation. These data suggest that RREB-1 may play a role in Ras and Raf signal transduction in medullary thyroid cancer and other cells.

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Abstract #1101 The Value of Calcitonin Calcium Stimulation Test Levels as Predictors of Medullary Thyroid Cancer.

Endocrine Practice ◽

10.1016/s1530-891x(20)47466-x ◽

2018 ◽

Vol 24 ◽

pp. 273-274

Author(s):

Corin Badiu ◽

Mara Baet ◽

Ruxandra Dobrescu ◽

Andra Caragheorgheopol ◽

Corneci Cristina

Keyword(s):

Thyroid Cancer ◽

Medullary Thyroid Cancer ◽

Stimulation Test ◽

Medullary Thyroid ◽

Calcium Stimulation

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Intraoperative and postoperative care of unsuspected pheochromocytoma in medullary thyroid cancer

Otolaryngology ◽

10.1016/s0194-5998(94)70538-0 ◽

1994 ◽

Vol 111 (5) ◽

pp. 665-668

Author(s):

S YEE ◽

S STERN

Keyword(s):

Thyroid Cancer ◽

Postoperative Care ◽

Medullary Thyroid Cancer ◽

Medullary Thyroid

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Immunoscintigraphy in Medullary Thyroid Cancer Using an 123I- or 111ln-Labelled Monoclonal Anti-CEA Antibody Fragment

Nuklearmedizin ◽

10.1055/s-0038-1624348 ◽

1986 ◽

Vol 25 (06) ◽

pp. 227-231 ◽

Cited By ~ 6

Author(s):

Chr. Eilles ◽

W. Spiegel ◽

W. Becker ◽

W. Börner ◽

Chr. Reiners

Keyword(s):

Bone Marrow ◽

Thyroid Cancer ◽

Potassium Iodide ◽

Medullary Thyroid Cancer ◽

Elevated Serum ◽

Antibody Fragment ◽

Special Importance ◽

Medullary Thyroid ◽

Medullary Cancer ◽

Serum Cea

The monoclonal anti-CEA F(ab’)2 fragment MAb BW 431/31, labelled with 123I or111 In, was used for immunoscintigraphy (IS) in 9 patients with medullary cancer of the thyroid (CCC). The results of 11 studies lead to the following conclusions: 1) When using radioiodine as a label for MAb in IS, potassium iodide is absolutely necessary to block the thyroid which is of special importance in patients with thyroid cancer; 2) Preinjection of “cold” MAb reduces the relatively high unspecific uptake (especially in bone marrow) of MAb BW 431/31, which is of special importance for the antibody labelled with 111 In; 3) IS with MAb BW 413/31 in patients with CCC and elevated serum CEA is positive only in cases with large secondaries; and 4) In patients with CCC and several manifestations of secondaries, only a single (large) metastasis may be apparent.

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