scholarly journals Separation and Determination of Fluorescein Isothiocyanate-Labeled Amino Acids by Capillary Electrophoresis with Laser-Induced Fluorescence Detection.

1998 ◽  
Vol 14 (5) ◽  
pp. 925-928 ◽  
Author(s):  
Keita TAKIZAWA ◽  
Hiroshi NAKAMURA
Keyword(s):  
Amino Acids ◽  
Capillary Electrophoresis ◽  
Fluorescence Detection ◽  
Fluorescein Isothiocyanate ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection

scholarly journals Several applications of capillary electrophoresis for wines analysis. Quantitation of organic and inorganic acids, inorganic cations, amino acids and biogenic amines

OENO One ◽  
1997 ◽  
Vol 31 (4) ◽  
pp. 213
Author(s):  
Michel Arellano ◽  
N. Siméon ◽  
Ph. Puig ◽  
François Couderc
Keyword(s):  
Amino Acids ◽  
Capillary Electrophoresis ◽  
Biogenic Amines ◽  
Fluorescence Detection ◽  
Fluorescein Isothiocyanate ◽  
Laser Induced Fluorescence ◽  
Inorganic Cations ◽  
Laser Induced Fluorescence Detection ◽  
Inorganic Acids ◽  
Relative Standard

<p style="text-align: justify;">Capillary electrophoresis is now a well known analytical mean for the analysis of a great number of different molecules in miscellaneous media. Its usefullness is relatively unknown in oenology.</p><p style="text-align: justify;">In this application note, we present the simultaneous analysis of organic and inorganic acids. Inorganic cations were also analysed. In both cases the detection was indirect-UVmode.</p><p style="text-align: justify;">Amino acids and biogenic amines were also separated and quantitated after derivation of the wine using the fluorescein isothiocyanate and laser induced fluorescence detection. Twenty eight amino acids and biogenic amines were identitied in the same run.</p><p style="text-align: justify;">The relative standard deviations of migration time and peak area allow very easy identification of the species and accute quantitations.</p>

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