scholarly journals The role of Mycobacterium tuberculosis complex strain on apoptosis and necroptosis state of macrophages derived from active pulmonary tuberculosis patients

2020 ◽  
Author(s):  
Budi Yanti ◽  
Mulyadi Mulyadi ◽  
Muhammad Amin ◽  
Harapan Harapan ◽  
Ni Made Mertaniasih ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Lung Damage ◽  
Beijing Strain ◽  
Death Domain ◽  
Tuberculosis Complex ◽  
Active Pulmonary Tuberculosis ◽  
Mtbc Strain ◽  
Apoptosis Level

Abstract Objective The role of Mycobacterium tuberculosis complex (MTBC) strain in tuberculosis (TB) infection in human is still questioned. The aim of this study was to determine whether MTBC strain is associated with apoptosis and necroptosis by measuring the expression of specific signaling pathways components (Fas-associated protein with death domain (FADD) and receptor interacting protein 3 (RIP3)), as well as the level of apoptosis. Results We recruited 24 TB patients infected with M. tuberculosis Beijing strain and six patients with M. bovis BCG strain. Data indicated that those who infected with M. tuberculosis were more frequent to have severe lung damage than M. bovis (odds ratio [OR]: 7.60; 95% confidence interval [CI]: 1.07-54.09). M. tuberculosis infection was also associated with lower expression of FADD and lower apoptosis level of macrophage cells compared to M. bovis . No significant different of RIP3 between strain groups. In conclusion, M. tuberculosis Beijing strain is associated with severe pulmonary damage, inhibits FADD expression and reduces apoptosis level in macrophages derived from TB. This suggests that MTBC strain potentially be used as determinant of progressivity of disease and tissue damage in TB cases.

scholarly journals The role of Mycobacterium tuberculosis complex strain on apoptosis and necroptosis state of macrophages derived from active pulmonary tuberculosis patients

2020 ◽  
Author(s):  
Budi Yanti ◽  
Mulyadi Mulyadi ◽  
Muhammad Amin ◽  
Harapan Harapan ◽  
Ni Made Mertaniasih ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Lung Damage ◽  
Beijing Strain ◽  
Death Domain ◽  
Tuberculosis Complex ◽  
Active Pulmonary Tuberculosis ◽  
Mtbc Strain ◽  
Apoptosis Level

Abstract Objective The role of Mycobacterium tuberculosis complex (MTBC) strain in tuberculosis (TB) infection in human is still questioned. The aim of this study was to determine whether MTBC strain is associated with apoptosis and necroptosis by measuring the expression of specific signaling pathways components (Fas-associated protein with death domain (FADD) and receptor interacting protein 3 (RIP3)), as well as the level of apoptosis.Results We recruited 24 TB patients infected with M. tuberculosis Beijing strain and six patients with M. bovis BCG strain. Data indicated that those who infected with M. tuberculosis were more frequent to have severe lung damage than M. bovis (odds ratio [OR]: 7.60; 95% confidence interval [CI]: 1.07-54.09). M. tuberculosis infection was also associated with lower expression of FADD and lower apoptosis level of macrophage cells compared to M. bovis . No significant different of RIP3 between strain groups. In conclusion, M. tuberculosis Beijing strain is associated with severe pulmonary damage, inhibits FADD expression and reduces apoptosis level in macrophages derived from TB. This suggests that MTBC strain potentially be used as determinant of progressivity of disease and tissue damage in TB cases.

scholarly journals The role of Mycobacterium tuberculosis complex species on apoptosis and necroptosis state of macrophages derived from active pulmonary tuberculosis patients

2020 ◽  
Author(s):  
Budi Yanti ◽  
Mulyadi Mulyadi ◽  
Muhammad Amin ◽  
Harapan Harapan ◽  
Ni Made Mertaniasih ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Lung Damage ◽  
Beijing Strain ◽  
Death Domain ◽  
Tuberculosis Complex ◽  
Complex Species ◽  
Pulmonary Tb ◽  
Apoptosis Level

Abstract Objective: The role of Mycobacterium tuberculosis complex (MTBC) species in tuberculosis (TB) infection in human is still questioned. The aim of this study was to determine whether M. tuberculosis and M. bovis is associated with apoptosis and necroptosis by measuring the expression of specific signaling pathways components (Fas-associated protein with death domain (FADD) and receptor interacting protein 3 (RIP3)), and the level of apoptosis. Results: We recruited 30 patients with pulmonary TB; 24 patients were infected with M. tuberculosis Beijing strain and six patients with M. bovis BCG strain. M. tuberculosis-infected patients were more likely to have severe lung damage compared to those infected with M. bovis (odds ratio [OR]: 7.60; 95% confidence interval [CI]: 1.07-54.09). M. tuberculosis infection was associated with lower expression of FADD and lower apoptosis level of macrophages compared to M. bovis. No significant different of RIP3 between MTBC species groups. In conclusion, M. tuberculosis Beijing strain was associated with severe pulmonary damage, inhibited FADD expression and reduced apoptosis level of macrophages derived from pulmonary TB patients. This suggests that the M. tuberculosis Beijing strain is potentially to be used as determinant of disease progressivity and tissue damage in TB cases.

scholarly journals The role of Mycobacterium tuberculosis complex species on apoptosis and necroptosis state of macrophages derived from active pulmonary tuberculosis patients

2020 ◽  
Author(s):  
Budi Yanti ◽  
Mulyadi Mulyadi ◽  
Muhammad Amin ◽  
Harapan Harapan ◽  
Ni Made Mertaniasih ◽  
...  
Keyword(s):  
Lung Damage ◽  
Beijing Strain ◽  
Death Domain ◽  
Interacting Protein ◽  
Complex Species ◽  
Active Pulmonary Tuberculosis ◽  
Pulmonary Damage ◽  
Species Groups ◽  
Apoptosis Level

Abstract Objective: The role of Mycobacterium tuberculosis complex (MTBC) species in tuberculosis (TB) infection in human is still questioned. The aim of this study was to determine whether M. tuberculosis and M. bovis is associated with apoptosis and necroptosis by measuring the expression of specific signaling pathways components (Fas-associated protein with death domain (FADD) and receptor interacting protein 3 (RIP3)), and the level of apoptosis.Results: We recruited 30 patients of pulmonary TB; 24 patients were infected with M. tuberculosis Beijing strain and six patients with M. bovis BCG strain. M. tuberculosis-infected patients were more likely to have severe lung damage compared to those infected with M. bovis (odds ratio [OR]: 7.60; 95% confidence interval [CI]: 1.07-54.09). M. tuberculosis infection was associated with lower expression of FADD and lower apoptosis level of macrophages compared to M. bovis. No significant different of RIP3 between MTBC species groups. In conclusion, M. tuberculosis Beijing strain was associated with severe pulmonary damage, inhibited FADD expression and reduced apoptosis level of macrophages derived from TB. This suggests that the M. tuberculosis Beijing strain is potentially to be used as determinant of disease progressivity and tissue damage in TB cases.

scholarly journals What is the role of the EUCAST reference method for MIC testing of the Mycobacterium tuberculosis complex?

2020 ◽  
Vol 26 (11) ◽  
pp. 1453-1455 ◽  
Author(s):  
Thomas Schön ◽  
Claudio Umberto Köser ◽  
Jim Werngren ◽  
Miguel Viveiros ◽  
Sophia Georghiou ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Reference Method ◽  
Tuberculosis Complex

The Role of Molecular Techniques for the Detection of Mycobacterium Tuberculosis Complex in Paraffin-embedded Biopsies

2019 ◽  
Vol 27 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Zaira Moure ◽  
Josep Castellví ◽  
Adrián Sánchez-Montalvá ◽  
Tomás Pumarola ◽  
M. Teresa Tórtola
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Molecular Techniques ◽  
Tuberculosis Complex

scholarly journals Role of GenoType® Mycobacterium Common Mycobacteria/Additional Species Assay for Rapid Differentiation between Mycobacterium tuberculosis Complex and Different Species of Non-tuberculous Mycobacteria

2013 ◽  
Vol 5 (02) ◽  
pp. 083-089 ◽  
Author(s):  
Amresh Kumar Singh ◽  
Anand Kumar Maurya ◽  
Jyoti Umrao ◽  
Surya Kant ◽  
Ram Awadh Singh Kushwaha ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Clinical Isolates ◽  
Molecular Diagnostic ◽  
Mycobacterium Fortuitum ◽  
Tuberculosis Complex ◽  
Accurate Identification ◽  
Additional Species ◽  
Culture Positive

ABSTRACT Background: Mycobacterium tuberculosis complex (MTBC) and non-tuberculous mycobacteria (NTM) may or may not have same clinical presentations, but the treatment regimens are always different. Laboratory differentiation between MTBC and NTM by routine methods are time consuming and cumbersome to perform. We have evaluated the role of GenoType® Mycobacterium common mycobacteria/additional species (CM/AS) assay for differentiation between MTBC and different species of NTM in clinical isolates from tuberculosis (TB) cases. Materials and Methods: A total of 1080 clinical specimens were collected from January 2010 to June 2012. Diagnosis was performed by Ziehl-Neelsen staining followed by culture in BacT/ALERT 3D system (bioMerieux, France). A total of 219 culture positive clinical isolates (BacT/ALERT® MP cultures) were selected for differentiation by p-nitrobenzoic acid (PNB) sensitivity test as and BIO-LINE SD Ag MPT64 TB test considering as the gold standard test. Final identification and differentiation between MTBC and different species of NTM were further confirmed by GenoType® Mycobacterium CM/AS assay (Hain Lifescience, Nehren, Germany). Results: Out of 219 BacT/ALERT® MP culture positive isolates tested by PNB as 153 MTBC (69.9%) and by GenoType® Mycobacterium CM/AS assay as 159 (72.6%) MTBC and remaining 60 (27.4%) were considered as NTM species. The GenoType® Mycobacterium CM/AS assay was proved 99.3% sensitive and 98.3% specific for rapid differentiation of MTBC and NTM. The most common NTM species were; Mycobacterium fortuitum 20 (33.3%) among rapid growing mycobacteria and Mycobacterium intracellulare 11 (18.3%) among slow growing mycobacteria. Conclusion: The GenoType® Mycobacterium assay makes rapid and accurate identification of NTM species as compared with different phenotypic and molecular diagnostic tool and helps in management of infections caused by different mycobacteria.

scholarly journals Evidence of the role of European wild boar as a reservoir of Mycobacterium tuberculosis complex

2008 ◽  
Vol 127 (1-2) ◽  
pp. 1-9 ◽  
Author(s):  
Victoria Naranjo ◽  
Christian Gortazar ◽  
Joaquín Vicente ◽  
José de la Fuente
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Wild Boar ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex ◽  
European Wild Boar
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