Changes of Tears MCP-1 Level After External Dacryocystorhinostomy in Primary Acquired Nasolacrimal Duct Obstruction

Purpose: To define tears MCP-1 changes after uncomplicated external dacryocystorhinostomy with bicanalicular silicone tube intubation surgery.Methods: In this prospective, longitudinal study, we included patients diagnosed with primary acquired nasolacrimal duct obstruction and underwent dacryocystorhinostomy with bicanalicular silicone tube intubation surgery. Tears samples were collected with a Schirmer strip placed lateral conjunctival cul-de-sac and stored in Eppendorf tubes at -80°C. At the end of the study, they were taken out of the -80°C cabinet and brought to room temperature. The papers were cut into small pieces and incubated in 2 milliliters tubes with phosphate-buffered saline solution in a shaker at 100 revolutions per minute. Then they were centrifuged at 1.000 revolutions per minute for 5 minutes. MCP-1 levels were determined by using an ELISA kit. Results: Of the patients, 14 (56%) were female, and 11 (44%) were male. The mean age was 52.7±10.3 years (ranged from 30 to 63 years). The MCP-1 levels were 498.66±101.35 ng/L, 576.40±149.78 ng/L, 422.53±85.94 ng/L, 436.96±81.38 ng/L before surgery, in the first week, the first and third months after surgery, respectively. The mean MCP-1 level significantly increased in the first week compared to the preoperative level (p < 0.001). In the postoperative first month, there was a prominent decrease (p < 0.001). In the third postoperative month, the mean MCP-1 level was not significantly increased compared to the postoperative first month (p=0.196).Conclusion: The tears MCP-1 level was significantly decreased after uncomplicated external dacryocystorhinostomy with bicanalicular silicone tube intubation surgery