A new pseudo-color coded optical system based on the liquid crystal spatial light modulator (LC-SLM) and a digital camera (CCD) is proposed. The SLM is used to replace the holographic grating with gray-scale image information, a gray-scale image in real-time modulation methods is proposed by synthesizing phase hologram and Ronchi grating, combined with the 4f coherent optical processing system and spatial filtering. For the high resolution gray image processed with existing digital pseudo-color method, the color sensitivity is low, algorithm is very complex. For traditional optical pseudo-color method, the gray scale image needs chemical pretreatment. The process is complex and time-consuming, and the real-time modulation could not be achieved. Our new method has enhanced the flexibility and adaptability of the optical pseudo-color, and give full play to the high sensitivity, high-capacity, rich colors and other features of the optical processing mode. At the same time, it overcomes the disadvantages of pure optical system which could not perform real-time processing. Therefore, it can be widely used in the field of remote sensing, biomedical, environmental monitoring, public security and criminal investigation, etc.
Real-Time Characterization of an Antimicrobial Mechanism-of-Action with Nonlinear Optical Scattering
