scholarly journals In Vitro Clonal Propagation of Vanilla (Vanilla planifolia ‘Andrews’)

HortScience ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 454-458 ◽  
Author(s):  
Hilda E. Lee-Espinosa ◽  
Joaquín Murguía-González ◽  
Benjamín García-Rosas ◽  
Ana L. Córdova-Contreras ◽  
Antonio Laguna-Cerda ◽  
...  
Keyword(s):  
Adventitious Shoots ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Vanilla Planifolia ◽  
Number Of Shoots

A complete and efficient regeneration protocol was developed for Vanilla planifolia ‘Andrews’, an endangered orchid species that represents an important crop in several tropical countries. Axillary buds excised from the first to the eighth node, considering the first to fourth nodes as “young” (zone 1) and the fifth to eighth as “mature” (zone 2), were cultured on Murashige and Skoog (MS) medium supplemented with 5.73, 7.64, 9.55, or 11.46 μm 6-benzylaminopurine (BA) for shoot induction and in combination with 4.45 μm naphthalene acetic acid (NAA) to induce multiple shoot proliferation. Cytokinin concentration and bud position in the stem had a significant effect on the number of shoots regenerated. The greatest shoot formation per explant, for the two tested zones, was obtained with 9.55 μm BA on MS medium supplemented with 100 mg·L−1 myoinositol, 150 mg·L−1 citric acid, 100 mg·L−1 ascorbic acid, and 20 g·L−1 sucrose. Young buds from zone 1 were able to form an average of 18.5 ± 2.4 shoots per explant, whereas buds from zone 2 induced a maximum of 11.0 ± 1.0 shoots per explant. Plants of 2 to 3 cm height developed a root system in half-strength MS medium supplemented with 0.44 μm NAA and, once they reached 5 cm height on average, were transferred to greenhouse conditions for their acclimatization where a 100% rate survival was achieved. The optimal use of both young and mature buds from each mother plant to induce adventitious shoots permitted a marked increase in the number of shoots per explant. By using all buds from the upper stem part (zone 1 + zone 2) and subculturing every 90 d, the multiplication rate was 1.1 to 1.86 × 105 shoots per bud per year.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Improved micropropagation and foliar micromorphological studies in Turnera ulmifolia L. – An important medicinal plant

2018 ◽  
Vol 30 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Mani Manokari ◽  
Mahipal S. Shekhawat
Keyword(s):  
Shoot Proliferation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Half Strength ◽  
Turnera Ulmifolia ◽  
Semi Solid ◽  
Number Of Shoots

Abstract The present study reports an efficient in vitro propagation system for Turnera ulmifolia using nodal segments as explants. Turnera ulmifolia (Passifloraceae) is an important garden plant with multipotent medicinal values. Effective shoot proliferation was achieved on agar gelled MS medium (Murashige and Skoog, 1962). The maximum number of shoots (8.3 ± 0.57) per initial explant was obtained on MS medium supplemented with 8.88 mM of 6-benzylaminopurine (BAP) and 0.54 mM of α-naphthalene acetic acid (NAA). The highest number of shoots (59.5 ± 2.10) proliferated on semi-solid MS medium (with agar) augmented with 2.22 mM of BAP and 2.32 mM of kinetin (Kin) along with 0.54 mM of NAA. Longer (4-5 cm) and healthy shoots were rooted (12.0 ± 0.10 roots per shoot) on half-strength MS medium fortified with 9.84 mM of indole-3 butyric acid (IBA). The in vitro regenerated plantlets were hardened in the greenhouse and transferred to the field. Significant developmental changes were observed in the foliar micromorphology of in vitro raised plantlets when these were transferred to the field. The stomatal index was gradually reduced (26.72 to 21.25) in the leaves from in vitro to field environments. But, vein-islets and veinlet terminations (13.4 and 7.6) were increased (39.7 and 18.4) respectively from in vitro to in vivo grown plants. Simple, unicellular, less frequent and underdeveloped trichomes were observed with the leaves of in vitro plants but fully developed trichomes recorded in the field transferred plants. The study could help in understanding the response and adaptation of tissue culture raised plantlets towards changed environmental conditions.

scholarly journals Effect of Growth Regulators on In Vitro Morphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. Using Mature Zygotic Embryos Explants

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Hussien H. Daffalla ◽  
Eltayb Abdellatef ◽  
Elsadig A. Elhadi ◽  
Mutasim M. Khalafalla
Keyword(s):  
Growth Regulators ◽  
Adventitious Shoots ◽  
Normal Growth ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Boscia Senegalensis

The percent study describes the in vitro responses of mature zygotic embryos of Boscia senegalensis to different concentrations (0.0–5.0 mg/L) of 6-benzyladnine (BA), Thidiazuron (TDZ), α-Naphthalene acetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) supplemented on Murashige and Skoog medium (MS). The plant growth regulators (PGRs) were considerably affected the morphogenetic responses. BA produced adventitious shoots through two ways: direct organogenesis and auxiliary shoot formation. Both 2, 4-D and TDZ tend to produce callus, whereas NAA improve the development of embryos to seedlings. Maximum number of shoots/explant (14.8 ± 0.6) was obtained on MS medium supplemented with 3.0 mg/L BA. 67.0% of excised shoots were rooted either on 1/2 MS medium augmented with or without 0.25 mg/L IBA. The highest number of roots (1.2 ± 0.4) and root length (0.5 ± 0.2 cm) was produced on 0.25 mg/L IBA-containing medium. Regenerated plants were successfully acclimatized and transferred to the green house with 70% survival rate. All the plants appeared morphologically uniform with normal growth pattern. A rapid (30 days), efficient and without subculturing protocol for in vitro regeneration of B. senegalensis was developed.

Rapid In Vitro Propagation of Bioenergy Crop Miscanthus Sacchariflorus

2012 ◽  
Vol 260-261 ◽  
pp. 181-186
Author(s):  
Hai Peng Guo ◽  
Ruo Xuan Shao ◽  
Chun Tao Hong ◽  
Heng Kang Hu ◽  
Bing Song Zheng ◽  
...  
Keyword(s):  
Adventitious Shoots ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Regeneration System ◽  
Bioenergy Feedstock ◽  
Shoot Apices ◽  
Rapid Propagation ◽  
Miscanthus Sacchariflorus

Miscanthus sacchariflorus is an important perennial bioenergy feedstock, but no information is available regarding plant rapid propagation from in vitro seed grown plantlets. The present study investigates the effects of the types and combination of plant growth regulators on tissue culture system of M. sacchariflorus. Shoot apices from in vitro germinated seedling explants were tested for adventitious shoot proliferation. The highest level of proliferation (proliferation coefficient 11.66) was obtained when shoot apices were cultured on Murashige and Skoog (MS) medium supplemented with 0.5 mg L−1 6-benzyladenine (BA), 0.05 mg L−1 α–naphthalene acetic acid (NAA), 3% sucrose, and 0.8% agar. The highest root number (13.33) and root length (9.67 cm) were obtained when adventitious shoots were cultured on half-strength MS medium supplemented with 0.4 mg L−1 NAA, 3% sucrose, and 0.8% agar. The efficient plant regeneration system developed here will be helpful for rapid propagation and further genetic improvement in M. sacchariflorus.

scholarly journals Micropropagation of Ilex glabra (L.) A. Gray

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 805-808 ◽  
Author(s):  
Youping Sun ◽  
Donglin Zhang ◽  
John Smagula
Keyword(s):  
Mass Production ◽  
Adventitious Roots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Axillary Bud ◽  
Nodal Segments ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Indolebutyric Acid

Nodal segments containing one axillary bud (1 to 1.5 cm) were disinfected using 10% bleach and were established on a Murashige and Skoog (MS) medium without hormones at 27 °C and with a 16-h photoperiod. The sprouted shoots (≈1.0 cm) were cultured on a MS medium supplemented with 6-benzylaminopurine (BAP), kinetin (KIN), or zeatin (ZT) at 2.3, 4.5, 9.1, or 18.2 μM. After 38 d, ZT and BAP significantly induced multiple shoot formation with multiplication rates of 4 to 6, whereas the multiplication rate of KIN was less than 2. Shoots cultured on ZT grew significantly taller than those on BAP and KIN. The height of the longest shoots treated with ZT was 4.6 cm, which was 1.6 to 2.2 times greater than those treated with BAP or KIN. To induce rooting, shoots (≈2 cm) were subcultured on one-fourth strength MS (1/4 MS) medium containing either 3-indolebutyric acid (IBA) or 1-naphthylacetic acid (NAA) at 2.6, 5.1, or 10.3 μM. Adventitious roots formed in vitro after 2 to 4 weeks. IBA at 10.3 μM produced the best rooting (100%) compared with other treatments after 38 d of culture. The average number of roots per shoot for IBA was ≈15, which was 1.6 to 3.1 times as many as that of other treatments. All rooted plantlets were then transplanted into a mix of peatmoss and perlite (1:1 v/v) and acclimatized in a mist system. Average plantlet survival was 73.6% after 35 d. After acclimatization, they were grown in a pot with Metro-mix under greenhouse conditions for 10 weeks where 95% of plants survived and grew up to 6.8 cm high. The micropropagation procedure, i.e., nodal segments containing one axillary bud proliferated on MS with 4.5 μM ZT followed by in vitro rooting on 1/4 MS plus 10.3 μM IBA, could be used for commercial mass production of new inkberry cultivars.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In-vitro development of Nauclea diderrichii (de Willd. & Th. Dur) Merrin liquid-M Smedia supplemented with benzyl amino purine (BAP) and naphthalene acetic acid (NAA)

2021 ◽  
Vol 24 (12) ◽  
pp. 2065-2069
Author(s):  
J.O. Afolabi ◽  
R.I. Oyediran ◽  
Y.O. Aguda ◽  
E.A. Adekunle
Keyword(s):  
Growth Regulators ◽  
Adventitious Shoots ◽  
Transition Process ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Temporary Immersion Bioreactor ◽  
Group A ◽  
Bioreactor System ◽  
Vitro Development

The growth of plantlets in Temporary Immersion Bioreactor system (TIBs) relies on initial successful liquid phase transition process. The response of N. diderrichii explants was assessed in liquid-M Smedia with a view to mass produce its seedlings using TIBs. Seven treatments consisting (A) 0.0/0.0, (B) 0.0/0.1, (C) 0.1/0.0, (D) 0.2/0.1, (E) 0.3/0.2, (F) 0.4/0.3 and (G) 0.5/0.4mg/lBAP/NAA combinations were studied. Each group consist of seven replicates and group A without Growth Regulators (GR) serves as control. The results at 4 Weeks after Inoculation (WAI) showed that effects of the growth regulators were significant on shoot length and number of adventitious shoots while number of roots and leaves were closely related. Treatment E produced highest number of adventitious shoots (3.6) which was higher than 0.9 shoots from treatment G and closely related to others. Maximum number of leaves (16.6) was produced by treatment F followed by E (15.7) while the least (12) was obtained in treatment A. The highest number of roots (4.9) was obtained from treatments B, followed by E (4.3) with the lowest being recorded in C (2.43). Liquid MS medium supplemented with 0.3/0.2mg/lBAP/NAA shows some promise for plantlets generation for the purpose of multiple shoot production of N. diderrichii in TIBs.

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

scholarly journals In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

1970 ◽  
Vol 17 ◽  
pp. 139-144 ◽  
Author(s):  
MS Rahman ◽  
MF Hasan ◽  
R Das ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

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