Genetic Linkage of Randomly Amplified Polymorphic DNA (RAPD) Markers in Sweetpotato

RAPD marker analyses were completed on parents and progeny of two sweetpotato [Ipomoea batatas (L.) Lam.] crosses to determine the feasibility of genetic linkage map construction. A total of 100 primers was tested and 96 produced amplified genomic DNA fragments. The average number of polymorphisms per primer was 0.69. A total of 134 polyphorphic markers was observed and 74 (60%) segregated 1 band present : 1 band absent as needed for use in genetic linkage mapping of polyploids. The 60% of RAPD markers that segregated 1:1 shows that genetic linkage mapping of the hexaploid sweetpotato by RAPD marker analysis is feasible. Linkage was determined for all markers that segregated 1:1 and five pairs of linked markers were found. These were the first linked molecular markers found in sweetpotato and they show that construction of a genetic linkage map is feasible. A genetic linkage map will be a valuable tool to assist in genetic improvements.

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Genetic linkage map construction for white jute (Corchorus capsularisL.) using SRAP, ISSR and RAPD markers

Plant Breeding ◽

10.1111/pbr.12205 ◽

2014 ◽

Vol 133 (6) ◽

pp. 777-781 ◽

Cited By ~ 13

Author(s):

Yanping Chen ◽

Liwu Zhang ◽

Jianmin Qi ◽

Hui Chen ◽

Aifen Tao ◽

...

Keyword(s):

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map ◽

Map Construction

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085 Development of a Cleaved Amplified pPolymorphic Sequence (CAP) and Restriction Fragment Length Polymorphisms (RFLPs) Linked to the Mi Root-knot Nematode (Meloidogyne incognita, race 1) Resistance Gene in Peach [Prunus persica (L.) Batsch]

HortScience ◽

10.21273/hortsci.35.3.403c ◽

2000 ◽

Vol 35 (3) ◽

pp. 403C-403

Author(s):

Anne M. Gillen ◽

Fredrick A. Bliss

Keyword(s):

Linkage Group ◽

Linkage Map ◽

Genetic Linkage ◽

Rapd Markers ◽

Genetic Linkage Map ◽

Prunus Persica ◽

Rapd Marker ◽

Rflp Markers ◽

Resistance Locus ◽

Root Knot Nematode

Peach rootstock breeding may be accelerated by utilization of molecular markers linked to the root-knot nematode resistance locus (Mi) to screen segregating populations. A genetic linkage map was constructed using RFLP markers in an F2 population (PMP2) that is segregating for this locus. PMP2 is derived from a controlled cross of the relatively diverse peach rootstocks Harrow Blood (susceptible) and Okinawa (homozygous resistant). Bulked Segregant Analysis was applied using RAPD markers. A single small (227 base pairs) RAPD marker was found to be linked to the dominant resistant allele of Mi at a distance of 10 cM. This new marker joined the Mi locus to the RFLP linkage map and showed that two dominant RFLP markers are located between the RAPD marker and Mi. RFLPS are expensive, time-consuming and RAPD markers are unreliable, and therefore both are unsuitable for screening breeding populations. We attempted to convert the RAPD marker to a more breeder-friendly CAPS marker. The converted CAP marker was dominant. Attempts to convert the CAP marker to a co-dominant marker were not successful. The utility of the CAP marker was tested in an open pollinated F2 population derived from the F1 parent of PMP2 and in several rootstocks. The genetic linkage map was compared to other Prunus maps. The PMP2 linkage group containing the Mi locus can be related to the peach × almond linkage group which contains the phosphoglucomutase Pgm-1 locus.

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