Genetic linkage map construction for white jute (Corchorus capsularisL.) using SRAP, ISSR and RAPD markers

2014 ◽  
Vol 133 (6) ◽  
pp. 777-781 ◽  
Author(s):  
Yanping Chen ◽  
Liwu Zhang ◽  
Jianmin Qi ◽  
Hui Chen ◽  
Aifen Tao ◽  
...  
1997 ◽  
Vol 122 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Paul. G. Thompson ◽  
Liang L. Hong ◽  
Kittipat Ukoskit ◽  
Zhiqiang Zhu

RAPD marker analyses were completed on parents and progeny of two sweetpotato [Ipomoea batatas (L.) Lam.] crosses to determine the feasibility of genetic linkage map construction. A total of 100 primers was tested and 96 produced amplified genomic DNA fragments. The average number of polymorphisms per primer was 0.69. A total of 134 polyphorphic markers was observed and 74 (60%) segregated 1 band present : 1 band absent as needed for use in genetic linkage mapping of polyploids. The 60% of RAPD markers that segregated 1:1 shows that genetic linkage mapping of the hexaploid sweetpotato by RAPD marker analysis is feasible. Linkage was determined for all markers that segregated 1:1 and five pairs of linked markers were found. These were the first linked molecular markers found in sweetpotato and they show that construction of a genetic linkage map is feasible. A genetic linkage map will be a valuable tool to assist in genetic improvements.


2011 ◽  
Vol 130 (6) ◽  
pp. 679-687 ◽  
Author(s):  
Mei-xia Chen ◽  
Cheng-lin Wei ◽  
Jian-min Qi ◽  
Xing-bo Chen ◽  
Jian-guang Su ◽  
...  

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 657-661 ◽  
Author(s):  
Y Yao ◽  
M Ban ◽  
J Brandle

To lay a foundation for molecular breeding efforts, the first genetic linkage map for Stevia rebaudiana has been constructed using segregation data from a pseudo test-cross F1 population. A total of 183 randomly amplified polymorphic DNA (RAPD) markers were analysed and assembled into 21 linkage groups covering a total distance of 1389 cM, with an average distance between markers of of 7.6 cM. The 11 largest linkage groups consisted of 4-19 loci, ranged in length from 56 to 174 cM, and accounted for 75% of the total map distance. Fifteen RAPD loci were found to be unlinked. From the 521 primers showing amplification products, 185 (35.5%) produced a total of 293 polymorphic fragments, indicating a high level of genetic diversity in stevia. Most of the RAPD markers in stevia segregated in normal Mendelian fashion.Key words: stevia, open-pollinated, genome map, RAPD.


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