scholarly journals In silico mining and characterization of simple sequence repeats (SSRs) from Euphorbia esula expressed sequence tags (ESTs): A potential crop for biofuel

Plant Omics ◽  
2017 ◽  
Vol 10 (02) ◽  
pp. 53-63 ◽  
Author(s):  
Surojit Sen ◽  
◽  
Budheswar Dehury ◽  
Jagajjit Sahu ◽  
Sunayana Rathi ◽  
...  
2011 ◽  
Vol 60 (1-6) ◽  
pp. 161-163 ◽  
Author(s):  
X. Yang ◽  
X. Sun ◽  
S. Zhang

Abstract Six simple sequence repeats (SSR) markers were developed from expressed sequence tags (ESTs) in the genus Larix. Based on evaluation with 49 L. kaempferi genotypes, the number of alleles per locus ranged from two to four, and the expected (He) and observed (Ho) heterozygosity values were 0.225−0.694 and 0.201−0.656, respectively. The inbreeding coffcient (FIS) for all loci were less than zero except that LAReSSR85 was 0.4383. All the six EST-SSR markers were transferable to L. gmelini, L. olgensis var Koreana, L. principisrupprechtii and L. olgensis. BlastX analysis showed that five of the EST-SSRs were homologous to known genes. The six EST-SSR markers developed here can be valuable for biological applications in Larix.


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