Effects of Nicotine and Its Withdrawal on The Postnatal Development of Rat Mitral Cells

Ayman Amer; Dorreia Zaghloul

doi:10.21608/eajbsd.2021.210041

Effects of Nicotine and Its Withdrawal on The Postnatal Development of Rat Mitral Cells

Egyptian Academic Journal of Biological Sciences D Histology & Histochemistry ◽

10.21608/eajbsd.2021.210041 ◽

2021 ◽

Vol 13 (2) ◽

pp. 135-150

Author(s):

Ayman Amer ◽

Dorreia Zaghloul

Keyword(s):

Postnatal Development ◽

Mitral Cells

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Postnatal development attunes olfactory bulb mitral cells to high-frequency signaling

Journal of Neurophysiology ◽

10.1152/jn.00315.2015 ◽

2015 ◽

Vol 114 (5) ◽

pp. 2830-2842 ◽

Cited By ~ 6

Author(s):

Yiyi Yu ◽

Shawn D. Burton ◽

Shreejoy J. Tripathy ◽

Nathaniel N. Urban

Keyword(s):

Olfactory Bulb ◽

Postnatal Development ◽

High Frequency ◽

Spike Timing ◽

Membrane Properties ◽

Mitral Cells ◽

Spike Time ◽

Biophysical Properties ◽

Potassium Conductances ◽

Stimulus Features

Mitral cells (MCs) are a major class of principal neurons in the vertebrate olfactory bulb, conveying odor-evoked activity from the peripheral sensory neurons to olfactory cortex. Previous work has described the development of MC morphology and connectivity during the first few weeks of postnatal development. However, little is known about the postnatal development of MC intrinsic biophysical properties. To understand stimulus encoding in the developing olfactory bulb, we have therefore examined the development of MC intrinsic biophysical properties in acute slices from postnatal day (P)7–P35 mice. Across development, we observed systematic changes in passive membrane properties and action potential waveforms consistent with a developmental increase in sodium and potassium conductances. We further observed developmental decreases in hyperpolarization-evoked membrane potential sag and firing regularity, extending recent links between MC sag heterogeneity and firing patterns. We then applied a novel combination of statistical analyses to examine how the evolution of these intrinsic biophysical properties specifically influenced the representation of fluctuating stimuli by MCs. We found that immature MCs responded to frozen fluctuating stimuli with lower firing rates, lower spike-time reliability, and lower between-cell spike-time correlations than more mature MCs. Analysis of spike-triggered averages revealed that these changes in spike timing were driven by a developmental shift from broad integration of inputs to more selective detection of coincident inputs. Consistent with this shift, generalized linear model fits to MC firing responses demonstrated an enhanced encoding of high-frequency stimulus features by mature MCs.

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