scholarly journals Monitoring genetic and metabolic potential for in situ bioremediation: Mass spectrometry. 1997 annual progress report

10.2172/13438 ◽  
1997 ◽  
Author(s):  
M.V. Buchanan ◽  
G.B. Hurst ◽  
P.F. Britt ◽  
S.A. McLuckey ◽  
M.J. Doktycz
Keyword(s):  
Mass Spectrometry ◽  
Progress Report ◽  
Metabolic Potential ◽  
In Situ Bioremediation ◽  
Annual Progress Report

scholarly journals Monitoring genetic and metabolic potential for in situ bioremediation: Mass spectrometry. 1998 annual progress report

10.2172/13439 ◽  
1998 ◽  
Author(s):  
M.V. Buchanan ◽  
G.B. Hurst ◽  
M.J. Doktycz ◽  
P.F. Britt ◽  
K. Weaver ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Progress Report ◽  
Metabolic Potential ◽  
In Situ Bioremediation ◽  
Annual Progress Report

scholarly journals Monitoring Genetic & Metabolic Potential for In Situ Bioremediation: Mass Spectrometry

1999 ◽  
Author(s):  
Michelle V Buchanan ◽  
Gregory B Hurst ◽  
Mary E Lidstrom ◽  
Anne Auman ◽  
Phillip F Britt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Metabolic Potential ◽  
In Situ Bioremediation

scholarly journals Monitoring Genetic and Metabolic Potential for In-Situ Bioremediation: Mass Spectrometry

2000 ◽  
Author(s):  
Michelle V Buchanan ◽  
Phillip F Britt ◽  
Mitchel J Doktycz ◽  
Gregory B Hurst ◽  
Mary E Lidstrom
Keyword(s):  
Mass Spectrometry ◽  
Metabolic Potential ◽  
In Situ Bioremediation

Diversity and distribution of Microcystis (Cyanobacteria) oligopeptide chemotypes from natural communities studied by single-colony mass spectrometry

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1785-1796 ◽  
Author(s):  
Martin Welker ◽  
Matthias Brunke ◽  
Karina Preussel ◽  
Indra Lippert ◽  
Hans von Döhren
Keyword(s):  
Mass Spectrometry ◽  
Principal Components ◽  
Direct Analysis ◽  
Clear Correlation ◽  
Metabolic Potential ◽  
Peptide Synthetase ◽  
Components Analysis ◽  
Peptide Data ◽  
Preliminary Classification

Microcystis sp. has been recognized in recent years as a producer of a high number of secondary metabolites. Among these, peptides that are produced by the non-ribosomal peptide synthetase pathway often show bioactivity or are toxic to humans. The production of particular peptides is specific for individual Microcystis clones, allowing their characterization as chemotypes by analysing the peptidome. The authors studied the in situ diversity of peptides and chemotypes in Microcystis communities from lakes in and around Berlin, Germany, by direct analysis of individual colonies by MALDI-TOF mass spectrometry. From 165 colonies analysed a total of 46 individual peptides could be identified, 21 of which have not been described previously. For six of the new peptides the structures could be elucidated from fragment patterns, while for others only a preliminary classification could be achieved. In most colonies, two to ten individual peptides were detected. In 19 colonies, 16 of which were identified as M. wesenbergii, no peptide metabolites could be detected. The peptide data of 146 colonies were subjected to an ordination (principal components analysis). The principal components were clearly formed by the microcystin variants Mcyst-LR, -RR and -YR, anabaenopeptins B and E/F, a putative microviridin, and a new cyanopeptolin. In the resulting ordination plots most colonies were grouped into five distinct groups, while 40 colonies scattered widely outside these groups. In some cases colonies from different lakes clustered closely, indicating the presence of similar chemotypes in the respective samples. With respect to colony morphology no clear correlation between a chemotype and a morphospecies could be established, but M. aeruginosa, for example, was found to produce predominantly microcystins. In contrast, M. ichthyoblabe colonies were mostly negative for microcystins and instead produced anabaenopeptins. The number of peptides detected in a limited number of samples and the various combinations of peptides in individual Microcystis colonies highlights the immense metabolic potential and diversity of this genus.

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