Purification, Crystallization and Preliminary X-Ray Diffraction Studies on Goat (Capra hircus) Hemoglobin - A Low Oxygen Affinity Species

2009 ◽  
Vol 16 (4) ◽  
pp. 454-456 ◽  
Author(s):  
Ponnuraj Moorthy ◽  
Kamariah Neelagandan ◽  
Moovarkumudalvan Balasubramanian ◽  
Mondikalipudur Ponnuswamy
Keyword(s):  
Oxygen Affinity ◽  
Capra Hircus ◽  
Low Oxygen ◽  
X Ray Diffraction ◽  
X Ray ◽  
Hemoglobin A ◽  
Low Oxygen Affinity

scholarly journals Crystallization and X-ray diffraction data analysis of human deoxyhaemoglobin A0 fully stripped of any anions

1999 ◽  
Vol 55 (11) ◽  
pp. 1914-1916 ◽  
Author(s):  
F. A. V. Seixas ◽  
W. F. de Azevedo ◽  
M. F. Colombo
Keyword(s):  
Diffraction Data ◽  
Oxygen Affinity ◽  
X Ray Diffraction ◽  
Structural Explanation ◽  
Human Haemoglobin ◽  
X Ray ◽  
High Resolution Structure ◽  
Allosteric Properties

In this work, initial crystallographic studies of human haemoglobin (Hb) crystallized in isoionic and oxygen-free PEG solution are presented. Under these conditions, functional measurements of the O2-linked binding of water molecules and release of protons have evidenced that Hb assumes an unforeseen new allosteric conformation. The determination of the high-resolution structure of the crystal of human deoxy-Hb fully stripped of anions may provide a structural explanation for the role of anions in the allosteric properties of Hb and, particularly, for the influence of chloride on the Bohr effect, the mechanism by which Hb oxygen affinity is regulated by pH. X-ray diffraction data were collected to 1.87 Å resolution using a synchrotron-radiation source. Crystals belong to the space group P21212 and preliminary analysis revealed the presence of one tetramer in the asymmetric unit. The structure is currently being refined using maximum-likelihood protocols.

HB Washtenaw [βT11(A8)VAL-PHE]: An Electrophoretically Silent, Unstable, Low Oxygen Affinity Variant Associated with Anemia and Chronic Cyanosis

Hemoglobin ◽  
1994 ◽  
Vol 18 (4-5) ◽  
pp. 285-295 ◽  
Author(s):  
K. Krishnan ◽  
F. Martinez ◽  
R. T. Wille ◽  
R. T. Jones ◽  
D. T. Shin ◽  
...  
Keyword(s):  
Oxygen Affinity ◽  
Low Oxygen ◽  
Low Oxygen Affinity

Long-term Room Temperature Instability in Thermal Conductivity of InGaZnO Thin Films

MRS Advances ◽  
2016 ◽  
Vol 1 (22) ◽  
pp. 1631-1636 ◽  
Author(s):  
Boya Cui ◽  
D. Bruce Buchholz ◽  
Li Zeng ◽  
Michael Bedzyk ◽  
Robert P. H. Chang ◽  
...  
Keyword(s):  
Thin Films ◽  
Thermal Conductivity ◽  
Room Temperature ◽  
Storage Time ◽  
Laser Deposition ◽  
Low Oxygen ◽  
X Ray Diffraction ◽  
3Ω Method ◽  
X Ray

ABSTRACTThe cross-plane thermal conductivities of InGaZnO (IGZO) thin films in different morphologies were measured on three occasions within 19 months, using the 3ω method at room temperature 300 K. Amorphous (a-), semi-crystalline (semi-c-) and crystalline (c-) IGZO films were grown by pulsed laser deposition (PLD), followed by X-ray diffraction (XRD) for evaluation of film quality and crystallinity. Semi-c-IGZO shows the highest thermal conductivity, even higher than the most ordered crystal-like phase. After being stored in dry low-oxygen environment for months, a drastic decrease of semi-c-IGZO thermal conductivity was observed, while the thermal conductivity slightly reduced in c-IGZO and remained unchanged in a-IGZO. This change in thermal conductivity with storage time can be attributed to film structural relaxation and vacancy diffusion to grain boundaries.

scholarly journals Hemoglobin Titusville: a rare low oxygen affinity hemoglobinopathy

2017 ◽  
Vol 5 (6) ◽  
pp. 1011-1012 ◽  
Author(s):  
Senan John Yasar ◽  
Vivian Irene Ravn Berg ◽  
Asim Ahmad ◽  
Donald Doll
Keyword(s):  
Oxygen Affinity ◽  
Low Oxygen ◽  
Low Oxygen Affinity

scholarly journals Haemoglobin caribbean β91 (F7) Leu → Arg: A mildly unstable haemoglobin with a low oxygen affinity

FEBS Letters ◽  
1976 ◽  
Vol 69 (1-2) ◽  
pp. 99-102 ◽  
Author(s):  
E. Ahern ◽  
A.V. Ahern ◽  
A.T. Hilton ◽  
G.R. Sergeant ◽  
B.E. Sergeant ◽  
...  
Keyword(s):  
Oxygen Affinity ◽  
Low Oxygen ◽  
Low Oxygen Affinity

Deactivation of Alumina Supported Catalysts Due to Spinel Formation

1994 ◽  
Vol 344 ◽  
Author(s):  
P. H. Bolt ◽  
M. E. Van Ipenburg ◽  
J. W. Geus ◽  
F. H. P. M. Habraken
Keyword(s):  
Transition Metal ◽  
Reaction Rate ◽  
Rutherford Backscattering Spectrometry ◽  
Supported Catalysts ◽  
Low Oxygen ◽  
X Ray Diffraction ◽  
X Ray ◽  
Spinel Formation ◽  
Transition Metal Element ◽  
Metal Element

AbstractAn important cause of deactivation of alumina supported transition metal (oxide) catalysts is a solid state reaction between the active component and the support. We therefore studied the hightemperature behavior of Me layers (Me = Co, Ni, Cu and Fe) on polycrystalline α-A12O3 and γ- Al2O3 substrates. The samples were first oxidized at moderate temperatures and then annealed at high temperatures (up to 1000 °C) in O2, N2, or N2/O2 mixtures. The interfacial reaction to MeA12O4 was assessed using Rutherford Backscattering Spectrometry and X-ray diffraction. The reaction rate strongly depends on the transition metal element Me: Fe < Ni < Co < Cu. Low oxygen pressures favour spinel formation. γ-A12O3 shows a much higher reactivity towards the MeOx overlayers than α-Al2O3.

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