Detection of SARS-CoV-2 in the sewerage system in Tunisia: a promising tool to confront COVID-19 pandemic

Future Virology ◽

10.2217/fvl-2021-0050 ◽

2021 ◽

Author(s):

Habib Jmii ◽

Hakima Gharbi-Khelifi ◽

Raouia Assaoudi ◽

Mahjoub Aouni

Keyword(s):

Quantitative Analysis ◽

Real Time ◽

Real Time Pcr ◽

Viral Rna ◽

Sewerage System ◽

Promising Tool ◽

Elution Method ◽

Second Period

Aim: The current study undertaken in Tunisia examines the use of wastewaters to monitor SARS-CoV-2 circulation. Materials & methods: Viral genetic materials collected in wastewaters during two different periods (September–October 2020 and February–April 2021) were concentrated using the adsorption-elution method. SARS-CoV-2 genes were researched by real-time PCR. Results: During the first period of the study, viral RNA was detected in 61.11% of the analyzed samples collected from Monastir city with a rate of 88.88% for raw wastewaters and 33.33% for treated wastewaters. Then, during the second period of the study, the quantitative analysis of wastewaters collected from seven governorates showed the presence of viral RNA among around 25% of them with variable RNA loads. The increased amounts of viral RNA detected in wastewaters were accompanied by an increase in the number of COVID-19 patients in Tunisia. Conclusion: Our results emphasize the importance of sewage survey in SARS-CoV-2 tracking.

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The quantitative analysis of intestinal microbiota by fluorescent real-time PCR as an alternative to bacteriological assays

10.26226/morressier.56d6be78d462b80296c97ad6 ◽

2016 ◽

Author(s):

Viktoria Lioudyno

Keyword(s):

Quantitative Analysis ◽

Real Time ◽

Intestinal Microbiota ◽

Real Time Pcr

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Persistence of SARS-CoV-2 Viral RNA in Nasopharyngeal Swabs after Death: An Observational Study

Microorganisms ◽

10.3390/microorganisms9040800 ◽

2021 ◽

Vol 9 (4) ◽

pp. 800

Author(s):

Francesca Servadei ◽

Silvestro Mauriello ◽

Manuel Scimeca ◽

Bartolo Caggiano ◽

Marco Ciotti ◽

...

Keyword(s):

Viral Load ◽

Observational Study ◽

Real Time ◽

Real Time Pcr ◽

Pcr Detection ◽

Viral Rna ◽

Clinical Documentation ◽

Post Mortem ◽

Medical Assistance ◽

Time Points

The aim of this study was to investigate the persistence of SARS-CoV-2 in post-mortem swabs of subjects who died from SARS-CoV-2 infection. The presence of the virus was evaluated post-mortem from airways of 27 SARS-CoV-2 positive patients at three different time points (T1 2 h; T2 12 h; T3 24 h) by real-time PCR. Detection of antibodies to SARS-CoV-2 was performed by Maglumi 2019-nCoV IgM/IgG chemiluminescence assay. SARS-CoV-2 viral RNA was still detectable in 70.3% of cases within 2 h after death and in 66,6% of cases up to 24 h after death. Our data showed an increase of the viral load in 78,6% of positive individuals 24 h post-mortem (T3) in comparison to that evaluated 2 h after death (T1). Noteworthy, we detected a positive T3 post-mortem swab (24 h after death) from 4 subjects who were negative at T1 (2 h after death). The results of our study may have an important value in the management of deceased subjects not only with a suspected or confirmed diagnosis of SARS-CoV-2, but also for unspecified causes and in the absence of clinical documentation or medical assistance.

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