For various proper inclusions of classes of groups [Formula: see text], we obtain a group [Formula: see text] and a first-order sentence φ such that H⊨φ but no G∈ C satisfies φ. The classes we consider include the finite, finitely presented, finitely generated with and without solvable word problem, and all countable groups. For one separation, we give an example of a f.g. group, namely ℤp ≀ ℤ for some prime p, which is the only f.g. group satisfying an appropriate first-order sentence. A further example of such a group, the free step-2 nilpotent group of rank 2, is used to show that true arithmetic Th(ℕ,+,×) can be interpreted in the theory of the class of finitely presented groups and other classes of f.g. groups.