scholarly journals Characterization of the 16S ribosomal RNA genes and phylogenetic relationships of sulfur-dependent thermoacidophilic archaebacteria.

1995 ◽  
Vol 41 (1) ◽  
pp. 75-81 ◽  
Author(s):  
NORIO KUROSAWA ◽  
KIYOTAKA OHKURA ◽  
TADAO HORIUCHI ◽  
YUKO H. ITOH
Keyword(s):  
Ribosomal Rna ◽  
Phylogenetic Relationships ◽  
Ribosomal Rna Genes ◽  
16S Ribosomal Rna ◽  
Rna Genes

scholarly journals Evaluation and utility of mitochondrial ribosomal genes for molecular systematics of parasitic nematodes

2020 ◽  
Author(s):  
Abigail Hui En Chan ◽  
Kittipong Chaisiri ◽  
Serge Morand ◽  
Naowarat Saralamba ◽  
Urusa Thaenkham
Keyword(s):  
Genetic Marker ◽  
Genetic Markers ◽  
Molecular Systematics ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
16S Ribosomal Rna ◽  
12S Rrna ◽  
Rrna Gene ◽  
12S Rrna Gene ◽  
Rna Genes

Abstract Background Molecular advances have accelerated our understanding of nematode systematics and taxonomy. However, comparative analyzes between various genetic markers have led to discrepancies in nematode phylogenies. This study aimed to evaluate the suitability of using mitochondrial 12S and 16S ribosomal RNA genes for nematode molecular systematics. Methods To study the suitability of mitochondrial 12S and 16S ribosomal RNA genes as genetic markers for nematode molecular systematics, we compared them with the other commonly used genetic markers, nuclear internal transcribed spacer 1 and 2 regions, nuclear 18S and 28S ribosomal RNA genes, and mitochondrial cytochrome c oxidase subunit 1 gene. After that, phylum-wide primers for mitochondrial 12S and 16S ribosomal RNA genes were designed, and parasitic nematodes of humans and animals from 75 taxa with 21 representative species were inferred through phylogenetic analyzes. Phylogenetic analyzes were carried out using maximum likelihood and Bayesian inference algorithms. Results The phylogenetic relationships of nematodes based on the mitochondrial 12S rRNA gene supported the monophyly of nematodes in clades I, IV, and V, reinforcing the potential of this gene as a genetic marker for nematode systematics. In contrast, the mitochondrial 16S rRNA gene only supported the monophyly of clades I and V, providing evidence that the 12S rRNA gene is more suitable for nematode molecular systematics. In this study, subclades of clade III containing various nematode families were not monophyletic when the 16S or 12S rRNA gene was used as the genetic marker. This is similar to the phylogenetic relationship revealed by previous studies using whole mitochondrial genomes as genetic markers. Conclusions This study supports the use of the 12S rRNA gene as a genetic marker for studying the molecular systematics of nematodes to understand intra-phyla relationships. Phylum-wide primers for nematodes using mitochondrial ribosomal genes were prepared, which may enhance future studies. Furthermore, sufficient genetic variation in the mitochondrial 12S and 16S rRNA genes between species also allowed for accurate taxonomy to species level, revealing the potential of these two genes as genetic markers for DNA barcoding.

Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment

Microbiology ◽  
1995 ◽  
Vol 141 (11) ◽  
pp. 2793-2800 ◽  
Author(s):  
W. D. Hiorns ◽  
R. C. Hastings ◽  
I. M. Head ◽  
A. J. McCarthy ◽  
J. R. Saunders ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
16S Ribosomal Rna ◽  
Ammonia Oxidizing Bacteria ◽  
Rna Genes

Chromosomal Mapping and Molecular Characterization of Ribosomal RNA Genes in Lebias fasciata (Teleostei, Cyprinodontidae)

Genetica ◽  
2004 ◽  
Vol 121 (1) ◽  
pp. 95-100 ◽  
Author(s):  
C. Tigano ◽  
L. Rocco ◽  
V. Ferrito ◽  
D. Costagliola ◽  
A. M. Pappalardo ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Chromosomal Mapping ◽  
Rna Genes
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