scholarly journals Over-Expression of Arabidopsis Phytochelatin Synthase Gene in Oryza Sativaconfers Enhanced Tolerance to Heavy Metal Cadmium

2019 ◽  
Vol 9 (1) ◽  
pp. 23-36
Author(s):  
Swathi Bai Moodavath et al., Swathi Bai Moodavath et al., ◽  
Keyword(s):  
Heavy Metal ◽  
Phytochelatin Synthase ◽  
Over Expression

scholarly journals (288) An Arabidopsis thaliana Phytochelatin Synthase (AtPCS1) Gene Expressed in Transgenic Indian Mustard Contributes Partial Tolerance to Cd and Zn

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1080E-1081
Author(s):  
Ksenija Gasic ◽  
Schuyler S. Korban
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Arabidopsis Thaliana ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Indian Mustard ◽  
35S Promoter ◽  
Phytochelatin Synthase ◽  
Mrna Accumulation ◽  
Transgenic Lines

Phytochelatins (PCs) are heavy metal binding peptides that play important roles in sequestration and detoxification of heavy metals in plants. To develop transgenic plants with increased tolerance and/or accumulation of heavy metals from soil, an Arabidopsis thaliana FLAG–tagged AtPCS1 cDNA encoding phytochelatin synthase (PCS) under the control of a 35S promoter was expressed in Indian mustard (Brassica juncea). Four transgenic Indian mustard lines, designated pc lines, with different levels of AtPCS1 mRNA accumulation and correspondent AtPCS1 protein levels were selected and analyzed for tolerance to cadmium (Cd) and zinc (Zn). Heavy metal tolerance was assessed by measuring root length of 10-day-old seedlings grown on agar medium supplemented with different concentrations of Cd (0, 100, 150, and 200 μm CdCl2) and Zn (200, 400, 600, and 800 μm ZnCl2). All transgenic lines showed significantly longer roots when grown on a medium supplemented with 100 μm CdCl2. No significant differences were observed between transgenic lines and wild type when plants were grown on higher levels of Cd. This indicated that only partial tolerance to Cd was observed in these transgenic lines. Similarly, partial tolerance for Zn was also observed in these transgenic lines, but up to levels of 400 μm ZnCl2. Expression levels of AtPCS1 protein were not related to tolerance responses for either Cd or Zn stresses in transgenic lines.

scholarly journals IMPROVING HEAVY METAL TOLERANCE OF YEAST BY TRANSFERRING A PHYTOCHELATIN SYNTHASE GENE FROM GARLIC

2005 ◽  
Vol 29 (4) ◽  
pp. 659-664
Author(s):  
JIANG Ying-Nan ◽  
◽  
FENG Bao-Min ◽  
ZHANG Hai-Yan ◽  
MA Mi
Keyword(s):  
Heavy Metal ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Phytochelatin Synthase

Over-expression of gsh1 in the cytosol affects the photosynthetic apparatus and improves the performance of transgenic poplars on heavy metal-contaminated soil

Plant Biology ◽  
2011 ◽  
Vol 13 (4) ◽  
pp. 649-659 ◽  
Author(s):  
L. A. Ivanova ◽  
D. A. Ronzhina ◽  
L. A. Ivanov ◽  
L. V. Stroukova ◽  
A. D. Peuke ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Contaminated Soil ◽  
Photosynthetic Apparatus ◽  
Over Expression ◽  
Transgenic Poplars

Chloroplast targeting of phytochelatin synthase in Arabidopsis: effects on heavy metal tolerance and accumulation

Biochimie ◽  
2006 ◽  
Vol 88 (11) ◽  
pp. 1743-1750 ◽  
Author(s):  
N. Picault ◽  
A.C. Cazalé ◽  
A. Beyly ◽  
S. Cuiné ◽  
P. Carrier ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Phytochelatin Synthase ◽  
Chloroplast Targeting

Enhancing the tolerance of zebrafish (Danio rerio) to heavy metal toxicity by the expression of plant phytochelatin synthase

2006 ◽  
Vol 122 (3) ◽  
pp. 316-325 ◽  
Author(s):  
Takafumi Konishi ◽  
Sachiko Matsumoto ◽  
Yusuke Tsuruwaka ◽  
Kentaro Shiraki ◽  
Kazumasa Hirata ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Danio Rerio ◽  
Metal Toxicity ◽  
Heavy Metal Toxicity ◽  
Phytochelatin Synthase

Expression of Ceratophyllum demersum phytochelatin synthase, CdPCS1, in Escherichia coli and Arabidopsis enhances heavy metal(loid)s accumulation

PROTOPLASMA ◽  
2013 ◽  
Vol 250 (6) ◽  
pp. 1263-1272 ◽  
Author(s):  
Devesh Shukla ◽  
Ravi Kesari ◽  
Manish Tiwari ◽  
Sanjay Dwivedi ◽  
Rudra Deo Tripathi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Ceratophyllum Demersum ◽  
Phytochelatin Synthase

scholarly journals Phytochelatin Synthase in Heavy Metal Detoxification and Xenobiotic Metabolism

2021 ◽  
Author(s):  
Ju-Chen Chia
Keyword(s):  
Heavy Metal ◽  
Xenobiotic Metabolism ◽  
Plant Performance ◽  
Biochemical Mechanism ◽  
Phytochelatin Synthase ◽  
Metal Detoxification ◽  
High Affinity ◽  
Heavy Metal Detoxification ◽  
Potential Applications ◽  
Gsh Metabolism

Phytochelatin synthase (PCS) is well-known for its role in heavy metal detoxification in plants, yeasts and non-vertebrate animals. It is a protease-like enzyme that catalyzes glutathione (GSH) to form phytochelatins (PCs), a group of Cys-rich and non-translational polypeptides with a high affinity to heavy metals. In addition, PCS also functions in xenobiotic metabolism by processing GS-conjugates in the cytosol. Because PCS is involved in GSH metabolism and the degradation of GS-conjugates, it is one of the important components in GSH homeostasis and GSH-mediated biodegradation. This chapter reviews the biochemical mechanism of PCS, how the enzyme activity is regulated, and its roles in heavy metal detoxification as well as GS-S-conjugate metabolism. This chapter also highlights the potential applications of PCS in the improvement of plant performance under combined stresses.

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