Expression of Ceratophyllum demersum phytochelatin synthase, CdPCS1, in Escherichia coli and Arabidopsis enhances heavy metal(loid)s accumulation

PROTOPLASMA ◽  
2013 ◽  
Vol 250 (6) ◽  
pp. 1263-1272 ◽  
Author(s):  
Devesh Shukla ◽  
Ravi Kesari ◽  
Manish Tiwari ◽  
Sanjay Dwivedi ◽  
Rudra Deo Tripathi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Ceratophyllum Demersum ◽  
Phytochelatin Synthase

Standardization and bacteria inhibitory test of purified extract of mahogany (Swietenia mahagoni (L.) Jacq) seeds and leaves

2019 ◽  
Vol 10 (3) ◽  
pp. 2132-2138
Author(s):  
Virsa Handayani ◽  
Rezki Amriati Syarif ◽  
Ahmad Najib ◽  
Aktsar Roskiana Ahmad ◽  
Abdullah Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Bitter Taste ◽  
Drying Shrinkage ◽  
Ash Content ◽  
Water Soluble ◽  
Lead Contamination ◽  
Specific Parameter ◽  
Chemical Content ◽  
Swietenia Mahagoni

Mahogany (Swietenia mahagoni (L.) Jacq) is one of the plants that is often used by the community as traditional medicine. One of them is antifungal, antibacterial, antidiabetic, and eczema. This study aims to obtain standardized extracts from mahogany seeds and leaves. Standardization of purified extract of mahogany has been carried out according to the monographs of extract standardization guidelines, which include testing of specific and non-specific parameters. The results of the specific parameter testing showed that the purified extract of mahogany seeds is a thick extract, brown to reddish, smells distinctive and has a bitter taste. While the purified extract of mahogany leaves is a thick extract, greenish-brown in color, distinctive smell and has a bitter taste. The chemical content of purified extract of mahogany seeds and leaves showed the presence of flavonoids, alkaloids, terpenoids and saponins. Water-soluble essence levels in mahogany seeds and leaves was 14.84% and 10.28%. While the ethanol-soluble essence levels in mahogany seeds and leaves were 15.38% and 12.43%. Testing of non-specific parameters on mahogany seeds and leaves showed the results of drying shrinkage levels of 0.22% and 8.84%, moisture content of 2.60% and 4.04%, total ash content of 1.71% and 1.93%, levels acidic insoluble ash 0.38% and 0.32%, Total Plate Number (ALT) of mahogany seed bacteria 1x102 colonies/g, Number of mahogany mold seeds 4x10 colonies/g, heavy metal lead contamination and cadmium in mahogany seeds 0.0607µg/g and<0.003µg/g. The inhibitory diameter of each concentration of seeds against Escherichia coli, 3%, 5%, 7%, and 9%, is 12,67; 13,67; 17,67; and 19,67 mm, respectively. The inhibitory diameter of each concentration of leaves against Escherichia coli, 3%, 5%, 7%, and 9%, is 10,27; 10,90; 13,46; and 15,68 mm, respectively.

scholarly journals Effects of various heavy metal nanoparticles on Enterococcus hirae and Escherichia coli growth and proton-coupled membrane transport

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Zaruhi Vardanyan ◽  
Vladimir Gevorkyan ◽  
Michail Ananyan ◽  
Hrachik Vardapetyan ◽  
Armen Trchounian
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Metal Nanoparticles ◽  
Membrane Transport ◽  
Enterococcus Hirae ◽  
Coli Growth

scholarly journals Modelling of Indicator Escherichia coli Contamination in Sentinel Oysters and Estuarine Water

2019 ◽  
Vol 16 (11) ◽  
pp. 1971 ◽  
Author(s):  
Saharuetai Jeamsripong ◽  
Edward R. Atwill
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Environmental Parameters ◽  
Ambient Air ◽  
Estuarine Water ◽  
Probable Number ◽  
Estuarine Waters ◽  
E Coli

This study was performed to improve the ability to predict the concentrations of Escherichia coli in oyster meat and estuarine waters by using environmental parameters, and microbiological and heavy metal contamination from shellfish growing area in southern Thailand. Oyster meat (n = 144) and estuarine waters (n = 96) were tested for microbiological and heavy metal contamination from March 2016 to February 2017. Prevalence and mean concentrations of E. coli were 93.1% and 4.6 × 103 most probable number (MPN)/g in oyster meat, and 78.1% and 2.2 × 102 MPN/100 mL in estuarine water. Average 7-day precipitation, ambient air temperature, and the presence of Salmonella were associated with the concentrations of E. coli in oyster meat (p < 0.05). Raw data (MPN/g of oyster meat and MPN/100 mL of estuarine water) and log-transformed data (logMPN/g of oyster meat and logMPN/100 mL of estuarine water) of E. coli concentrations were examined within two contrasting regression models. However, the more valid predictions were conducted using non-log transformed values. These findings indicate that non-log transformed data can be used for building more accurate statistical models in microbiological food safety, and that significant environmental parameters can be used as a part of a rapid warning system to predict levels of E. coli before harvesting oysters.

scholarly journals Utilização de macrófitas aquáticas de três diferentes tipos ecológicos para remoção de Escherichia coli de efluentes de criação de pacu

2017 ◽  
Vol 22 (4) ◽  
pp. 657-663 ◽  
Author(s):  
Argos Willian de Almeida Assunção ◽  
Pedro Gatti Junior ◽  
Renata Vilar Almeida ◽  
Yuri Gasparotto ◽  
Luiz Augusto do Amaral
Keyword(s):  
Escherichia Coli ◽  
Constructed Wetland ◽  
Eichhornia Crassipes ◽  
Ceratophyllum Demersum ◽  
Typha Domingensis ◽  
Piaractus Mesopotamicus ◽  
E Coli

RESUMO O presente trabalho avaliou a contaminação por Escherichia coli em viveiro de criação de pacu (Piaractus mesopotamicus) e sua remoção do efluente utilizando um sistema do tipo constructed wetland, contendo macrófitas aquáticas de três tipos ecológicos diferentes (Eichhornia crassipes, Ceratophyllum demersum e Typha domingensis). Durante três meses foram realizadas análises do número mais provável (NMP) de Escherichia coli na água de abastecimento do viveiro, no seu efluente e em cada etapa do tratamento (sedimentação e emprego de cada espécie de macrófita). Concluiu-se que a contaminação por E. coli na água de abastecimento e no viveiro pode alcançar o ambiente por meio do efluente. O sistema de tratamento proposto foi eficiente na remoção de E. coli da água, sendo que, após estabilização do sistema, ocorreu remoção média de 95% da contaminação. A etapa contendo E. crassipes apresentou maior remoção média, porém mostrou diminuição da eficiência a partir de 60 dias após implementação do sistema. A remoção média das etapas contendo C. demersum e T. domingensis foi prejudicada devido à presença de E. coli no solo usado como substrato durante as primeiras semanas de experimento. Contudo, com a diminuição do efeito da contaminação presente no substrato, essas duas etapas foram fundamentais para manutenção da qualidade do efluente tratado, demonstrando que o consórcio de espécies de tipos ecológicos diferentes foi vantajoso, uma vez que a qualidade da água do efluente foi mantida mesmo quando algumas etapas do tratamento diminuíram sua eficiência.

Detection of heavy metal toxicity and genotoxicity in wastewaters by microbial assay

1994 ◽  
Vol 30 (10) ◽  
pp. 145-151 ◽  
Author(s):  
J. C. Codina ◽  
A. Pérez-García ◽  
A. de Vicente
Keyword(s):  
Escherichia Coli ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Metal Toxicity ◽  
Ames Test ◽  
Deionized Water ◽  
Toxicity Tests ◽  
Water Solutions ◽  
Microbial Assay ◽  
Higher Sensitivity

The effect of wastewater on the sensitivity of toxicity and genotoxicity assays for the detection of heavy metals was evaluated. Five microbiological toxicity tests were used to compare the toxicity of cadmium, copper, chromium, mercury, nickel, and zinc, both in deionized water solutions and in wastewater. The toxicity assays employed were: Microtox®, two tests of growth inhibition using Pseudomonas fluorescens, and two spectrophotometric assays of the inhibition of respiration test using baker's yeast and P. fluorescens. Also, the genotoxic effect of the assayed metals was evaluated by using the Ames test, the Escherichia coli WP2 test, and the SOS test. The sensitivity to metals decreases in most of the toxicity and genotoxicity assays; in general, higher sensitivity thresholds (EC20) in wastewater than in water solutions were determined. Each test shows different sensitivities to each metal, which is related to different sensitivities of the organisms used in the assays, as well as to other factors.

scholarly journals Crystal structure of the CusBA heavy-metal efflux complex of Escherichia coli

Nature ◽  
2011 ◽  
Vol 470 (7335) ◽  
pp. 558-562 ◽  
Author(s):  
Chih-Chia Su ◽  
Feng Long ◽  
Michael T. Zimmermann ◽  
Kanagalaghatta R. Rajashankar ◽  
Robert L. Jernigan ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Escherichia Coli ◽  
Heavy Metal ◽  
Metal Efflux ◽  
Heavy Metal Efflux
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