Genetic resources of relict populations of Pinus sylvestris (L.) in Western Carpathians assessed by chloroplast microsatellites

Abstract The major drawback of microsatellites analysis is that they are expensive to develop, labor-intensive and demand skilled personnel. However, such studies might be still simplified and accelerated by multiplexing of the markers and the use of highthroughput systems for genotyping DNA fragments. In this paper we present a single, simple and highly effective PCRmultiplex reaction composed of six chloroplast microsatellites widely used for population studies in pines but here applied to Pinus sylvestris. The reaction allows for rapid genotyping of large sample sizes.

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Structure of Pinus sylvestris L. populations in Bulgaria revealed by chloroplast microsatellites and terpenes analysis: Provenance tests

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2005.07.011 ◽

2005 ◽

Vol 33 (12) ◽

pp. 1226-1245 ◽

Cited By ~ 23

Author(s):

Krassimir D. Naydenov ◽

Francine M. Tremblay ◽

Alexander Alexandrov ◽

Nicole J. Fenton

Keyword(s):

Pinus Sylvestris ◽

Chloroplast Microsatellites

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Allozyme variation of seed embryos and mating system in relict populations of scots pine (Pinus sylvestris L.) from the Kremenets Hill Ridge and Maloe Poles’e

Russian Journal of Genetics ◽

10.1134/s1022795411070106 ◽

2011 ◽

Vol 47 (7) ◽

pp. 829-835 ◽

Cited By ~ 2

Author(s):

I. I. Korshikov ◽

L. A. Kalafat ◽

A. N. Lisnichuk ◽

T. I. Velicoridko ◽

E. A. Mudrik

Keyword(s):

Pinus Sylvestris ◽

Mating System ◽

Scots Pine ◽

Allozyme Variation ◽

Relict Populations

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Nowe stanowiska i historia odkrycia Saxifraga paniculata (Saxifragaceae) na Wyżynie Krakowsko-Częstochowskiej

Fragmenta Floristica et Geobotanica Polonica ◽

10.35535/ffgp-2020-0047 ◽

2020 ◽

pp. 684-689

Author(s):

Zbigniew Szeląg

Keyword(s):

Rock Climbing ◽

Western Carpathians ◽

Relict Populations ◽

Tatra Mts

In May 2018, three new populations of Saxifraga paniculata Mill. were found in the Skały Kroczyckie hills in the Wyżyna Krakowsko-Częstochowska Upland (Fig. 1). In Poland, S. paniculata is frequent only in the Tatra Mts and Pieniny Mts in the Western Carpathians; its occurrence in the Wyżyna Krakowsko-Częstochowska Upland consists of relict populations from the Pleistocene glacial (Vistulian). In the Wyżyna Krakowsko-Częstochowska Upland, Saxifraga paniculata occurs in four regions: the Dolina Będkowska valley, Olsztyn village, Podzamcze village and Skały Kroczyckie hills. The oldest locality was found in Olsztyn village by Professor Wojciech Jastrzębowski in 1847. The locality in Ojców village has not been confirmed for more than 150 years. All populations of S. paniculata in the Wyżyna Krakowsko-Częstochowska Upland are strongly endangered due to intensive tourism and rock climbing.

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