Pathological threshold of human sperm DNA oxidation, an increase value for infertility diagnostics

2017 ◽  
Author(s):  
SOLENE VORILHON
Keyword(s):  
Human Sperm ◽  
Dna Oxidation ◽  
Sperm Dna

scholarly journals Human sperm DNA oxidation, motility and viability in the presence of l-carnitine during in vitro incubation and centrifugation

Andrologia ◽  
2011 ◽  
Vol 44 ◽  
pp. 505-512 ◽  
Author(s):  
S. Banihani ◽  
R. Sharma ◽  
M. Bayachou ◽  
E. Sabanegh ◽  
A. Agarwal
Keyword(s):  
Human Sperm ◽  
Dna Oxidation ◽  
In Vitro Incubation ◽  
Sperm Dna

scholarly journals Accuracy of human sperm DNA oxidation quantification and threshold determination using an 8-OHdG immuno-detection assay

2018 ◽  
Vol 33 (4) ◽  
pp. 553-562 ◽  
Author(s):  
S Vorilhon ◽  
F Brugnon ◽  
A Kocer ◽  
S Dollet ◽  
C Bourgne ◽  
...  
Keyword(s):  
Human Sperm ◽  
Dna Oxidation ◽  
Threshold Determination ◽  
Detection Assay ◽  
Sperm Dna

Use of laptop computers connected to internet through Wi-Fi decreases human sperm motility and increases sperm DNA fragmentation

2012 ◽  
Vol 97 (1) ◽  
pp. 39-45.e2 ◽  
Author(s):  
Conrado Avendaño ◽  
Ariela Mata ◽  
César A. Sanchez Sarmiento ◽  
Gustavo F. Doncel
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Human Sperm ◽  
Sperm Dna Fragmentation ◽  
Laptop Computers ◽  
Sperm Dna

DETERMINATION OF THE HUMAN SPERM DNA FRAGMENTATION INDEX

Vestnik ◽  
2021 ◽  
pp. 226-232
Author(s):  
К. К. Тлеуханов ◽  
Н. А. Алтыбаева ◽  
М. К. Отарбаев ◽  
Е. М. Тойшибеков ◽  
А. А. Тлеуханова
Keyword(s):  
Dna Fragmentation ◽  
Human Sperm ◽  
Sperm Dna Fragmentation ◽  
Fragmentation Index ◽  
Sperm Dna ◽  
Sperm Dna Fragmentation Index ◽  
Dna Fragmentation Index

В статье представлены собранные данные о методах устранения повышенной частоты фрагментации ДНК у сперматозоидов, которые в некоторых исследованиях подтверждают, что введение антиоксидантов может снизить уровень фрагментации ДНК у сперматозоидов. The article presents collected data on methods of eliminating the increased frequency of DNA fragmentation in spermatozoa, which in some studies confirm that the introduction of antioxidants can reduce the level of DNA fragmentation in spermatozoa.

DNA methylation in human sperm: a systematic review

2020 ◽  
Vol 26 (6) ◽  
pp. 841-873 ◽  
Author(s):  
Fredrika Åsenius ◽  
Amy F Danson ◽  
Sarah J Marzi
Keyword(s):  
Systematic Review ◽  
Dna Methylation ◽  
Environmental Factors ◽  
Pregnancy Outcomes ◽  
Human Sperm ◽  
Semen Parameters ◽  
Dna Methylome ◽  
Sperm Dna ◽  
Offspring Health

Abstract BACKGROUND Studies in non-human mammals suggest that environmental factors can influence spermatozoal DNA methylation, and some research suggests that spermatozoal DNA methylation is also implicated in conditions such as subfertility and imprinting disorders in the offspring. Together with an increased availability of cost-effective methods of interrogating DNA methylation, this premise has led to an increasing number of studies investigating the DNA methylation landscape of human spermatozoa. However, how the human spermatozoal DNA methylome is influenced by environmental factors is still unclear, as is the role of human spermatozoal DNA methylation in subfertility and in influencing offspring health. OBJECTIVE AND RATIONALE The aim of this systematic review was to critically appraise the quality of the current body of literature on DNA methylation in human spermatozoa, summarize current knowledge and generate recommendations for future research. SEARCH METHODS A comprehensive literature search of the PubMed, Web of Science and Cochrane Library databases was conducted using the search terms ‘semen’ OR ‘sperm’ AND ‘DNA methylation’. Publications from 1 January 2003 to 2 March 2020 that studied human sperm and were written in English were included. Studies that used sperm DNA methylation to develop methodologies or forensically identify semen were excluded, as were reviews, commentaries, meta-analyses or editorial texts. The Grading of Recommendations, Assessment, Development and Evaluations (GRADE) criteria were used to objectively evaluate quality of evidence in each included publication. OUTCOMES The search identified 446 records, of which 135 were included in the systematic review. These 135 studies were divided into three groups according to area of research; 56 studies investigated the influence of spermatozoal DNA methylation on male fertility and abnormal semen parameters, 20 studies investigated spermatozoal DNA methylation in pregnancy outcomes including offspring health and 59 studies assessed the influence of environmental factors on spermatozoal DNA methylation. Findings from studies that scored as ‘high’ and ‘moderate’ quality of evidence according to GRADE criteria were summarized. We found that male subfertility and abnormal semen parameters, in particular oligozoospermia, appear to be associated with abnormal spermatozoal DNA methylation of imprinted regions. However, no specific DNA methylation signature of either subfertility or abnormal semen parameters has been convincingly replicated in genome-scale, unbiased analyses. Furthermore, although findings require independent replication, current evidence suggests that the spermatozoal DNA methylome is influenced by cigarette smoking, advanced age and environmental pollutants. Importantly however, from a clinical point of view, there is no convincing evidence that changes in spermatozoal DNA methylation influence pregnancy outcomes or offspring health. WIDER IMPLICATIONS Although it appears that the human sperm DNA methylome can be influenced by certain environmental and physiological traits, no findings have been robustly replicated between studies. We have generated a set of recommendations that would enhance the reliability and robustness of findings of future analyses of the human sperm methylome. Such studies will likely require multicentre collaborations to reach appropriate sample sizes, and should incorporate phenotype data in more complex statistical models.

Dynamic assessment of human sperm DNA damage I: the effect of seminal plasma-sperm co-incubation after ejaculation

2018 ◽  
Vol 50 (8) ◽  
pp. 1381-1388 ◽  
Author(s):  
Eva Tvrdá ◽  
Francisca Arroyo ◽  
Jaime Gosálvez
Keyword(s):  
Dna Damage ◽  
Seminal Plasma ◽  
Dynamic Assessment ◽  
Human Sperm ◽  
Sperm Dna Damage ◽  
Sperm Dna
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