Amniotic membrane transplantation (AMT) without the use of sutures/fibrin glue

Introduction: Amniotic membrane transplantation (AMT) has been used for a wide variety of ocular surface problems. Current techniques for the AMT involve suturing of the graft or patch over the ocular surface or use of fibrin glue.Objective: To describe a new sutureless technique of amniotic membrane transplantation (AMT) without the use of sutures/fibrin glue.Materials and methods: Amniotic membrane transplantation as overlay or patch was done in fourteen eyes of eight patients with severe dry eye due to Stevens Johnson syndrome for ocular surface reconstruction, promotion of epithelization, reduction of inflammation and symptomatic relief from dry eye. In this new technique of amniotic membrane transplantation, we mount the amniotic membrane with its epithelial side up on a conformer & place it on the ocular surface without applying any sutures.Conclusion: In all the fourteen eyes of eight patients operated at our centre, the amniotic membrane take up was satisfactory, with significant reduction of inflammation and symptomatic relief from dry eye. This is a simple, fast effective technique with surgical outcomes comparable to amniotic membrane transplantation using sutures/ fibrin glue.

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Amniotic membrane transplantation for ocular surface reconstruction in Stevens-Johnson syndrome

Ophthalmology ◽

10.1016/s0161-6420(00)00026-9 ◽

2000 ◽

Vol 107 (5) ◽

pp. 975-979 ◽

Cited By ~ 67

Author(s):

Santosh G Honavar ◽

Aashish K Bansal ◽

Virender S Sangwan ◽

Gullapalli N Rao

Keyword(s):

Surface Reconstruction ◽

Amniotic Membrane ◽

Ocular Surface ◽

Stevens Johnson Syndrome ◽

Amniotic Membrane Transplantation ◽

Ocular Surface Reconstruction ◽

Johnson Syndrome

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Amniotic Membrane With Living Related Corneal Limbal/Conjunctival Allograft for Ocular Surface Reconstruction in Stevens-Johnson Syndrome

Archives of Ophthalmology ◽

10.1001/archopht.121.10.1369 ◽

2003 ◽

Vol 121 (10) ◽

pp. 1369 ◽

Cited By ~ 46

Author(s):

José A. P. Gomes

Keyword(s):

Surface Reconstruction ◽

Amniotic Membrane ◽

Ocular Surface ◽

Stevens Johnson Syndrome ◽

Ocular Surface Reconstruction ◽

Johnson Syndrome ◽

Living Related

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Amniotic Membrane Transplantation for Ocular Surface Reconstruction

Bioscience Reports ◽

10.1023/a:1017995810755 ◽

2001 ◽

Vol 21 (4) ◽

pp. 481-489 ◽

Cited By ~ 104

Author(s):

S.C.G. Tseng

Keyword(s):

Surface Reconstruction ◽

Amniotic Membrane ◽

Ocular Surface ◽

Transforming Growth Factor ◽

Ease Of Use ◽

Myoblast Differentiation ◽

Amniotic Membrane Transplantation ◽

Clinical Effects ◽

Epithelial Stem Cells ◽

Ocular Surface Reconstruction

The use of amniotic membrane (or amnion) for transplantation as graft in ocular surface reconstruction is reviewed. This technique has become widespread because of the availability of the amnion, convenience and ease of use, and high and reproducible success rates. The mechanisms of action of the transplantation are varied and include the prolongation and clonogenic maintenance of epithelial progenitor cells, promotion of goblet and non-goblet cell differentiation, exclusion of inflammatory cells with anti-protease activities, suppression of Transforming Growth Factor β signaling and myoblast differentiation of normal fibroblasts. The observed clinical effects include facilitation of epithelialization, maintenance of normal phenotypes, and reduction of inflammation, vascularization and scarring. Amniotic membrane transplantation is being increasingly used as graft for various conjunctival and corneal diseases and as a patch in cases of chemical and thermal burns, refractory and recalcitrant keratitis, and most recently as an excellent substrate for expanding epithelial stem cells ex vivo.

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