Development and validation of reverse transcriptase-PCR assays for the testing of SARS-CoV-2

2020 ◽  
Vol COVID-19 ◽  
pp. Accepted versio ◽  
Author(s):  
Kanti Pabbaraju ◽  
Anita A. Wong ◽  
Mark Douesnard ◽  
Raymond Ma ◽  
Kara Gill ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcriptase Pcr ◽  
Pcr Assays ◽  
Development And Validation

scholarly journals Development of Reverse Transcriptase PCR Assays for Detection of Active Human Herpesvirus 6 Infection

2001 ◽  
Vol 39 (6) ◽  
pp. 2308-2310 ◽  
Author(s):  
G. Van den Bosch ◽  
G. Locatelli ◽  
L. Geerts ◽  
G. Faga ◽  
M. Ieven ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Reverse Transcriptase Pcr ◽  
Pcr Assays ◽  
Herpesvirus 6

Commercial Real-Time Reverse Transcriptase PCR Assays Can Underestimate or Fail to Quantify Hepatitis Delta Virus Viremia

2013 ◽  
Vol 11 (6) ◽  
pp. 734-740 ◽  
Author(s):  
Ségolène Brichler ◽  
Frédéric Le Gal ◽  
Afifaa Butt ◽  
Sylvie Chevret ◽  
Emmanuel Gordien
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Hepatitis Delta Virus ◽  
Hepatitis Delta ◽  
Reverse Transcriptase Pcr ◽  
Pcr Assays ◽  
Delta Virus

scholarly journals Immunomagnetic Separation Combined with Real-Time Reverse Transcriptase PCR Assays for Detection of Norovirus in Contaminated Food

2008 ◽  
Vol 74 (13) ◽  
pp. 4226-4230 ◽  
Author(s):  
YoungBin Park ◽  
You-Hee Cho ◽  
YoungMee Jee ◽  
GwangPyo Ko
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Immunomagnetic Separation ◽  
Rt Pcr ◽  
Reverse Transcriptase Pcr ◽  
Food Borne ◽  
Contaminated Food ◽  
Microbial Contaminants ◽  
Pcr Assays ◽  
Sensitive Method

ABSTRACT We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.

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