Use of recombinant human erythropoietin to avoid blood transfusion in a Jehovah's Witness requiring hemispherectomy

1993 ◽  
Vol 79 (4) ◽  
pp. 600-602 ◽  
Author(s):  
Steven J. Schiff ◽  
Steven L. Weinstein
Keyword(s):  
Blood Transfusion ◽  
Recombinant Human Erythropoietin ◽  
Recombinant Erythropoietin ◽  
Content Type ◽  
Jehovah’S Witness ◽  
Human Recombinant Erythropoietin ◽  
Jehovah's Witness ◽  
Human Erythropoietin ◽  
Witness Patient ◽  
Fine Print

✓ The use of perioperative human recombinant erythropoietin is described in a Jehovah's Witness patient. Despite significant anemia, the child's hematocrit was sufficiently increased by the use of erythropoietin so that a two-stage hemispherectomy could be performed without blood transfusion.

scholarly journals Major liver surgery in a Jehovah’s Witness patient: challenges for safe surgery

2019 ◽  
pp. 24-26
Author(s):  
WY Lim ◽  
L Loh ◽  
SR Desai ◽  
SL Tien ◽  
BK Goh ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Patient Autonomy ◽  
Ethical Issues ◽  
Major Surgery ◽  
Blood Products ◽  
Jehovah’S Witness ◽  
Jehovah's Witness ◽  
Safe Surgery ◽  
Patient Consent ◽  
Witness Patient

Jehovah’s Witnesses presenting for major surgery run the risk of major bleeding, which is complicated by the refusal to accept blood transfusion. We present a case of a 63-year-old woman, a Jehovah’s Witness, who was diagnosed with hepatocellular carcinoma and advised for curative laparoscopic liver segmentectomy. Due to the risk of significant intraoperative haemorrhage, her perioperative care was coordinated in a multidisciplinary manner. Informed consent requires the physician to advise on the material risks of undertaking major surgery without blood transfusion and the possible alternatives. Conflicting ethical issues of patient autonomy and beneficence related to refusal of blood products also arise. Perioperative strategies to minimise blood loss, maximise haematopoiesis and tolerance of anaemia to facilitate safe surgery in such patients are also presented. Written patient consent obtained.

scholarly journals Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent

2019 ◽  
Vol 12 (1) ◽  
pp. 103-107
Author(s):  
Marta Ostrihoňová ◽  
Jana Adamíková ◽  
Tomáš Molnár ◽  
Monika Antošová ◽  
Milan Polakovič
Keyword(s):  
Cation Exchange ◽  
Chromatographic Separation ◽  
Recombinant Human Erythropoietin ◽  
Batch Adsorption ◽  
Recombinant Erythropoietin ◽  
Optimal Conditions ◽  
Effect Of Ph ◽  
Human Recombinant Erythropoietin ◽  
Human Erythropoietin ◽  
Nacl Concentration

Abstract This work deals with the capture of human recombinant erythropoietin (rhEPO) from a mixture of proteins in a concentrated postcultivation supernatant. Cation-exchange multimodal adsorbent Capto MMC ImpRes was selected as potential chromatographic separation material. Its equilibrium properties were investigated in batch adsorption experiments. The effect of pH in the range of 5.5—7.5 and NaCl concentration in the range of 0—300 mM on the adsorption of rhEPO and contaminant proteins was examined. Optimal conditions found in these equilibrium experiments were applied to rhEPO adsorption in a chromatographic column. Several experiments were carried out at different elution conditions to optimize the rhEPO yield and selectivity.

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