In-vitro development of early preantral ovarian follicles from postpuberal mice in a simplified culture system

2005 ◽  
Vol 20 (1) ◽  
pp. 1-4
Author(s):  
Hidemi Kada
Keyword(s):  
Culture System ◽  
Ovarian Follicles ◽  
In Vitro Development ◽  
Vitro Development

Stage specific expression of cell cycle genes during in vivo or in vitro development of ovarian follicles in sheep

2016 ◽  
Vol 143 ◽  
pp. 1-7 ◽  
Author(s):  
V. Praveen Chakravarthi ◽  
S.S.R. Kona ◽  
A.V.N. Siva Kumar ◽  
M. Bhaskara ◽  
V.H. Rao
Keyword(s):  
Cell Cycle ◽  
Ovarian Follicles ◽  
Specific Expression ◽  
In Vitro Development ◽  
Cell Cycle Genes ◽  
Vitro Development

Effect of morphological integrity, period, and type of culture system on the in vitro development of isolated caprine preantral follicles

2014 ◽  
Vol 82 (2) ◽  
pp. 312-317 ◽  
Author(s):  
A.F.C. Pessoa ◽  
R.M.P. Rocha ◽  
I.R. Brito ◽  
G.M. Silva ◽  
R.N. Chaves ◽  
...  
Keyword(s):  
Culture System ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Vitro Development

scholarly journals Delineation of precursors in murine spleen that develop in contact with splenic endothelium to give novel dendritic-like cells

Blood ◽  
2010 ◽  
Vol 115 (18) ◽  
pp. 3678-3685 ◽  
Author(s):  
Jonathan K. H. Tan ◽  
Pravin Periasamy ◽  
Helen C. O'Neill
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Culture System ◽  
In Vitro Development ◽  
Cell Lineages ◽  
Mhc Ii ◽  
Murine Spleen ◽  
Vitro Development

Abstract Hematopoietic cell lineages are best described in terms of distinct progenitors with limited differentiative capacity. To distinguish cell lineages, it is necessary to define progenitors and induce their differentiation in vitro. We previously reported in vitro development of immature dendritic-like cells (DCs) in long-term cultures (LTCs) of murine spleen, and in cocultures of spleen or bone marrow (BM) over splenic endothelial cell lines derived from LTCs. Cells produced are phenotypically distinct CD11bhiCD11cloCD8−MHC-II− cells, tentatively named L-DCs. Here we delineate L-DC progenitors as different from known DC progenitors in BM and DC precursors in spleen. The progenitor is contained within the lineage-negative (Lin)−c-kit+ subset in neonatal and adult spleen. This subset has multipotential reconstituting ability in mice. In neonatal spleen, the progenitor is further enriched within the c-kitlo and CD34+ subsets of Lin−c-kit+ cells. These cells seed cocultures of splenic endothelial cells, differentiating to give L-DCs that can activate T cells. L-DC progenitors are distinguishable from described splenic CD11clo DC precursors and from Fms-like tyrosine kinase 3+ DC progenitors in BM. Overall, this study confirms that LTCs are a physiologically relevant culture system for in vitro development of a novel DC type from spleen progenitors.

Effect of leptin on in vitro development of ovine preantral ovarian follicles

2016 ◽  
Vol 85 (2) ◽  
pp. 224-229 ◽  
Author(s):  
P. Kamalamma ◽  
S.S.R. Kona ◽  
V. Praveen Chakravarthi ◽  
A.V.N. Siva Kumar ◽  
B. Punyakumari ◽  
...  
Keyword(s):  
Ovarian Follicles ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals Simulated Microgravity Using a Rotary Culture System Compromises the In Vitro Development of Mouse Preantral Follicles

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151062 ◽  
Author(s):  
Shen Zhang ◽  
Dahan Zheng ◽  
Yonggen Wu ◽  
Wei Lin ◽  
Zaichong Chen ◽  
...  
Keyword(s):  
Culture System ◽  
Simulated Microgravity ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Vitro Development ◽  
Rotary Culture

132 EFFECTS OF OXYGEN TENSION, MEDIUM, AND WELL OF THE WELL ON IN VITRO DEVELOPMENT OF THE MOUSE EMBRYO

2011 ◽  
Vol 23 (1) ◽  
pp. 170
Author(s):  
Y. S. Li ◽  
Z. B. Cao ◽  
Y. Liu ◽  
H. G. Cao ◽  
Y. Tao ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Culture System ◽  
Total Cell ◽  
Mouse Embryos ◽  
In Vitro Development ◽  
Cell Numbers ◽  
Gas Phases ◽  
Blastocyst Rate ◽  
Vitro Development

The objectives of the present research were to investigate whether embryo culture media have preferences for oxygen tension, to explore the feasibility of using physical lung air to support the in vitro development of mouse embryos, and to evaluate the effect of well of the well (WOW) culture on in vitro preimplantational development of mouse embryos. The results are as follows. First, cleavage rate and blastocyst rate were not significantly different between medium CZB and mKSOM regardless of using 3 gas phases: 4% CO2 + 16% O2 + 78% N2 + 2% H2O (lung air), 5% CO2 + 5% O2 + 90% N2 (5% O2, low oxygen), and 5% CO2 + 95% air (20% O2, high oxygen; P > 0.05), but mean total cell numbers per blastocyst cultured in CZB medium were higher than those in mKSOM when the lung air was used (P < 0.05). Second, when mKSOM was used as the basic medium, the blastocyst rate (22.6%) in the 5% O2 gas phase was notably higher than that in other 2 gas phases (P < 0.05). Third, for the CZB medium, the blastocyst rate was not different significantly among 3 gas environments (P > 0.05). Fourth, both the blastocyst rate (74.6 ± 5.1%) and the mean total cell numbers per blastocyst (76 ± 2) cultured in the WOW system were obviously higher than those in the group culture system (38.2 ± 6.6% and 58 ± 4). Taken together, our results indicate that mKSOM and CZB have preferences for oxygen tension during in vitro culture of mouse embryos, the lung air was reaffirmed to be able to effectively support in vitro preimplantation development of mouse embryos, and the WOW culture system can apparently enhance in vitro developmental competence and blastocyst quality of mouse embryos. L. YS, C. ZB: equal contribution; supported by NSFC (30700574), 863 (2008AA101003).

Sign in / Sign up

Export Citation Format

Share Document