ABSTRACTPseudonocardia dioxanivoransstrain BERK-1 grows aerobically with 1,4-dioxane as its sole substrate. Reported here is its draft genome sequence, with a size of 7.1 Mbp. Key genes are highlighted in this article. BERK-1 exhibits a reduced level of cell aggregation and adherence to surfaces compared to those ofP. dioxanivoransCB1190, giving it an apparent advantage for movement through soil.