scholarly journals Identification and Molecular Characterization of the Chloroplast Targeting Domain of Turnip yellow mosaic virus Replication Proteins

2017 ◽  
Vol 8 ◽  
Author(s):  
Lucille Moriceau ◽  
Lucile Jomat ◽  
Stéphane Bressanelli ◽  
Catherine Alcaide-Loridan ◽  
Isabelle Jupin
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Virus Replication ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Replication Proteins ◽  
Chloroplast Targeting

Characterization of the virus encoded subunit of turnip yellow mosaic virus rna replicase

Virology ◽  
1986 ◽  
Vol 152 (2) ◽  
pp. 322-330 ◽  
Author(s):  
T. Candresse ◽  
C. Mouches ◽  
J.M. Bove
Keyword(s):  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Virus Rna

scholarly journals Assembly of Turnip Yellow Mosaic Virus Replication Complexes: Interaction between the Proteinase and Polymerase Domains of the Replication Proteins

2004 ◽  
Vol 78 (15) ◽  
pp. 7945-7957 ◽  
Author(s):  
Anna Jakubiec ◽  
Julien Notaise ◽  
Vincent Tournier ◽  
François Héricourt ◽  
Maryse A. Block ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Chloroplast Envelope ◽  
Physical Interaction ◽  
Helicase Domain ◽  
Genome Replication ◽  
Turnip Yellow Mosaic Virus ◽  
Replication Proteins ◽  
Polymerase Domain ◽  
Positive Strand Rna

ABSTRACT Turnip yellow mosaic virus (TYMV), a positive-strand RNA virus in the alphavirus-like supergroup, encodes two nonstructural replication proteins (140K and 66K), both of which are required for its RNA genome replication. The 140K protein contains domains indicative of methyltransferase, proteinase, and NTPase/helicase activities, while the 66K protein encompasses the RNA-dependent RNA polymerase domain. Recruitment of the 66K protein to the sites of viral replication, located at the periphery of chloroplasts, is dependent upon the expression of the 140K protein. Using antibodies raised against the 140K and 66K proteins and confocal microscopy, we report the colocalization of the TYMV replication proteins at the periphery of chloroplasts in transfected or infected cells. The replication proteins cofractionated in functional replication complexes or with purified chloroplast envelope membranes prepared from infected plants. Using a two-hybrid system and coimmunoprecipitation experiments, we also provide evidence for a physical interaction of the TYMV replication proteins. In contrast to what has been found for other members of the alphavirus-like supergroup, the interaction domains were mapped to the proteinase domain of the 140K protein and to a large region encompassing the core polymerase domain within the 66K protein. Coexpression and colocalization experiments confirmed that the helicase domain of the 140K protein is unnecessary for the proper recruitment of the 66K protein to the chloroplast envelope, while the proteinase domain appears to be essential for that process. These results support a novel model for the interaction of TYMV replication proteins and suggest that viruses in the alphavirus-like supergroup may have selected different pathways to assemble their replication complexes.

Molecular Characterization of Bean yellow mosaic virus from Vegetatively Propagated Dwarf Gentiana Plants

2002 ◽  
Vol 68 (4) ◽  
pp. 378-381 ◽  
Author(s):  
Hiroyuki UGA ◽  
Yuki O. KOBAYASHI ◽  
Kyoji HAGIWARA ◽  
Yohachiro HONDA ◽  
Toshihiro OMURA ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus

scholarly journals Characterization of Chimeric Turnip Yellow Mosaic Virus Genomes That Are Infectious in the Absence of Aminoacylation

Virology ◽  
1997 ◽  
Vol 230 (1) ◽  
pp. 113-124 ◽  
Author(s):  
Joel B. Goodwin ◽  
James M. Skuzeski ◽  
Theo W. Dreher
Keyword(s):  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Virus Genomes

scholarly journals Identification and molecular characterization of Bean yellow mosaic virus infecting French bean in Himachal Pradesh

VirusDisease ◽  
2015 ◽  
Vol 26 (4) ◽  
pp. 315-318 ◽  
Author(s):  
P. N. Sharma ◽  
Vivek Sharma ◽  
Anuradha Sharma ◽  
Kajal Rajput ◽  
S. K. Sharma
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
French Bean ◽  
Himachal Pradesh ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus
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